scholarly journals Effects of different anticoagulants on glycated albumin quantification

2018 ◽  
Vol 29 (1) ◽  
pp. 138-141 ◽  
Author(s):  
Graziella Bonetti ◽  
Nicola Di Gaetano ◽  
Renata Paleari ◽  
Ferruccio Ceriotti
Keyword(s):  
Glycated Albumin ◽  
Biological Variation ◽  
Wilcoxon Test ◽  
Clinical Use ◽  
Test Results ◽  
Diabetes Screening ◽  
Asian Countries ◽  
Sample Tube ◽  
First Time ◽  
Paired Wilcoxon Test

Introduction: In the last 20 years glycated albumin (GA) measurement has been demonstrated to be a reliable glycation marker and recently as the most innovative one in western countries. Glycated albumin has been already adopted by some Asian countries due to its usefulness in diabetes screening. The aim of the present study was to investigate for the first time the effects of different anticoagulants on GA assay. Materials and methods: From each of 60 patients a serum tube and K3EDTA, Li-Heparin and NaF-EDTA containing tubes were collected. All tubes were from Sarstedt (Verona, Italy). Glycated albumin was measured in duplicate in each sample tube in a single analytical run with quantILab glycated albumin (Instrumentation Laboratory SpA - A Werfen Company, Milan, Italy) on Architect c8000 analyser (Abbott SRL, Rome, Italy). Comparison of GA% in evaluated tubes was made by paired Wilcoxon test. Results: Median and interquartile range GA% concentrations were 15.4% (13.2 - 19.1) in serum, 15.7% (13.6 - 19.9) in K3EDTA, 15.6% (13.3 - 19.7) in Li-heparin and 15.5% (13.1 - 19.3) in NaF-EDTA samples, respectively. Glycated albumin mean relative bias respect to serum was within desirable bias derived from biological variation studies (± 2.9%) when K3EDTA (+ 2.8%), Li-heparin (+ 0.9%) or NaF-EDTA (+ 0.1%), were used as anticoagulants. Conclusions: Our results demonstrate that the GA% assay is not affected by relevant interferences when K3EDTA, Li-heparin or NaF-EDTA are used as anticoagulants, so they can be used interchangeably without a relevant impact on the clinical use of the test.

First data on the biological variation and quality specifications for plasma ammonia concentrations in healthy subjects

2016 ◽  
Vol 54 (5) ◽  
Author(s):  
Fatma Ucar ◽  
Gonul Erden ◽  
Seyda Ozdemir ◽  
Nurgul Ozcan ◽  
Erdem Bulut ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Plasma Sample ◽  
Biological Variation ◽  
Test Results ◽  
Healthy Individuals ◽  
Variation Data ◽  
Quality Specifications ◽  
Collection Period ◽  
First Time ◽  
Analytical Variation

AbstractBackground:Most of the factors causing preanalytical and analytical variation in ammonia measurement have been identified. Biological variation data for ammonia is still lacking. We therefore estimated the components of biological variation (within-subject=CVMethods:Blood samples from 20 healthy subjects were collected in K2EDTA tubes daily over a period of 4 consecutive days from each subject. Each plasma sample was split into two aliquots; one was immediately analyzed as the samples were collected and the other was stored –80 °C until testing at the end of the collection period and analyzed at once in one analytical run. All samples were analyzed in duplicate. Estimations were calculated according to Fraser and Harris methods.Results:CVConclusions:The present study for the first time described the components of biological variation for ammonia in healthy individuals. These data regarding biological variation of ammonia could be useful for a better evaluation of ammonia test results in clinical interpretation and for determining quality specifications based on biological variation.

Biological variation of glycated albumin, glucose and albumin in healthy Turkish subjects

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Osman Oğuz ◽  
Hilal Mercan ◽  
F. Sinem Hocaoglu-Emre
Keyword(s):  
Coefficient Of Variation ◽  
Glycated Albumin ◽  
Biological Variation ◽  
Test Results ◽  
Routine Test ◽  
Short Term ◽  
Standardized Protocol ◽  
Reference Change Value ◽  
Index Of Individuality ◽  
Term Monitoring

AbstractObjectivesBiological variation (BV) in laboratory tests can be defined as the variation in analyte concentration over time within and between individuals. Glycated albumin (GA) is a ketoamine which is used in the short-term monitoring of diabetes. The aim of this research was to determine BV of GA, glucose, and albumin under a well-designed and standardized protocol.MethodsBlood samples were collected weekly from 21 healthy subjects (10 males, 11 females) for four consecutive weeks. Samples were analyzed using enzymatic methods in duplicate. After subjected to outlier and normality tests, variables as the within-subject biologic coefficient of variation (CVI) and between-subject biologic coefficient of variation (CVG), the index of individuality (II), and reference change value (RCV) were calculated.ResultsAnalytical coefficient of variation (CVA) was 3.5, 1.78, and 2.9%, for GA, glucose and albumin, respectively. The estimates for CVI and CVG: GA: 4.1%, 6.3%; glucose: 3.8%, 4.8%; albumin: 3.5%, 4%. RCVs and IIs were: 15%, 0.60; 12%, 0.79; 13%, 0.9 for GA, glucose and albumin, respectively.ConclusionsThe BV data of GA derived from this study might be applied to understand routine test results better and establish the quality standards for the analyte.

scholarly journals The effect of salinity on enterocytozoon hepatopenaei infection in Penaeus vannamei under experimental conditions

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
L. F. Aranguren Caro ◽  
F. Alghamdi ◽  
K. De Belder ◽  
J. Lin ◽  
H. N. Mai ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Oral Route ◽  
Western Hemisphere ◽  
Penaeus Vannamei ◽  
Asian Countries ◽  
Experimental Conditions ◽  
Reliable Source ◽  
Specific Pathogen ◽  
Enterocytozoon Hepatopenaei ◽  
First Time

Abstract Background Enterocytozoon hepatopenaei (EHP) is an enteric pathogen that affects Penaeus vannamei and Penaeus monodon shrimp in many SE Asian countries. In the western hemisphere, EHP was reported for the first time in 2016 in farmed P. vannamei in Venezuela. Anecdotal evidence suggests that EHP is more prevalent in grow-out ponds where the salinity is high (> 15 parts per thousand (ppt)) compared to grow-out ponds with low salinities (< 5 ppt). Considering that P. vannamei is an euryhaline species, we were interested in knowing if EHP can propagate in P. vannamei in low salinities. Results In this study, we described an experimental infection using fecal strings as a source inoculum. Specific Pathogen Free (SPF) P. vannamei were maintained at three different salinities (2 ppt, 15 ppt, and 30 ppt) while continuously challenged using feces from known EHP-infected P. vannamei over a period of 3 weeks. The fecal strings, used as a source of EHP inocula in the challenges, was sufficient to elicit an infection in shrimp maintained at the three salinities. The infectivity of EHP in shrimp reared at 2 ppt, 15 ppt, and 30 ppt salinities was confirmed by PCR and histopathology. The prevalence and the severity of the EHP infection was higher at 30 ppt than at 2 ppt and 15 ppt. Conclusion The data suggests that fecal strings are a reliable source of EHP inoculum to conduct experimental challenges via the fecal-oral route. An EHP infection can occur at a salinity as low as 2 ppt, however, the prevalence and the severity of the EHP infection is higher at a salinity of 30 ppt.

scholarly journals COT-13 Current situation and problems of cancer genomic profiling test in Kyoto University Hospital

2020 ◽  
Vol 2 (Supplement_3) ◽  
pp. ii22-ii22
Author(s):  
Yoshiki Arakawa ◽  
Junko Suga ◽  
Yukinori Terada ◽  
Kohei Nakajima ◽  
Masahiro Tanji ◽  
...  
Keyword(s):  
Treatment Options ◽  
Insurance Coverage ◽  
Germline Mutations ◽  
University Hospital ◽  
Genomic Profiling ◽  
Test Results ◽  
Final Test ◽  
First Time ◽  
To Receive ◽  
Secondary Finding

Abstract Objective: Kyoto University Hospital has introduced the cancer genomic profiling tests, Oncoprime in 2015, Guardant360 in 2018, which are not under insurance coverage, FoundationOne CDx(F1CDx) and OncoGuide NCC Oncopanel system(NCC OP) in 2019, which received approval for insurance coverage for the first time in Japan. We investigated the results of cancer genomic profiling test under insurance coverage in our hospital. Methods: A special facility for the cancer genomic profiling tests was produced. To perform the cancer genomic profiling test, an outpatient must visit the facility three times (learning, ordering of the test, and getting the results). The expert panels decide the final test results and treatment options with the all information of the patients. Results: From November 2019 to March 2020, 51 and 9 patients were tested with F1CDx and NCC OP, respectively. 16 patients (31%) of F1CDX and 2 patients (22%) of NCC OP got treatment recommendations from the expert panels. However, only 5 patients (9.8%) of F1CDX and 1 patient (11%) of NCC OP received the treatments. The secondary finding suspecting germline mutations was found in 8 patients of F1CDX. Conclusion: After the approval the cancer genomic profiling tests with insurance coverage in Japan, it becomes easy for the patients to perform the test and get the genetic information of the tumor. However, it remains not easy to receive the recommended drugs because of several limitations of their usages.

Improving the Strength of Abrasive Wheels and Optimizing the Control over the Strength of Composite Abrasive Materials

2019 ◽  
Vol 946 ◽  
pp. 380-385
Author(s):  
Boris A. Chaplygin ◽  
Viacheslav V. Shirokov ◽  
Tat'yana A. Lisovskaya ◽  
Roman A. Lisovskiy
Keyword(s):  
Mechanical Properties ◽  
Test Results ◽  
Key Factors ◽  
Special Equipment ◽  
Material Density ◽  
Factors Affecting ◽  
Control Factors ◽  
Reinforcing Material ◽  
Special Studies ◽  
First Time

The strength of abrasive wheels is one of the key factors affecting the performance of abrasive machining. The paper discusses ways to improve the strength of abrasive wheels. The stress-state mathematical model presented herein is a generalization of the existing models. It is used herein to find for the first time that there are numerous optimal combinations of the elastic modulus and reinforcing material density, which result in the same minimum value of the objective function. It is found out that increasing the radius of the reinforcing component while also optimizing the mechanical properties of its material may increase the permissible breaking speed of the wheel several times. We herein present a regression equation and a nomogram for finding the optimal combination of control factors. Conventional methods for testing the mechanical properties of materials, which have been proven reliable for testing metals and alloys, are not as reliable for testing abrasive materials, as the test results they generate are not sufficiently stable or accurate. We therefore propose an alternative method that does not require any special equipment or special studies.

Ambient vibration test results of the Imperial County Services Building

1983 ◽  
Vol 73 (6A) ◽  
pp. 1895-1902
Author(s):  
Gerard C. Pardoen
Keyword(s):  
Strong Motion ◽  
Ambient Vibration ◽  
Vibration Test ◽  
Test Results ◽  
Structural Failure ◽  
Imperial Valley ◽  
Ambient Vibration Test ◽  
Significant Interest ◽  
First Time ◽  
County Services

Abstract The ambient vibration test results conducted on the Imperial County Services Building prior to the 15 October 1979 Imperial Valley earthquake are summarized. These results are of significant interest because the Imperial County Services Building has been the source of many postearthquake investigations due to the fact that the 1979 earthquake represented the first time a building instrumented with strong motion recorders suffered and recorded the major structural failure.

Answering the Questions of the Psychologist Assessing the Visually Handicapped Child

1975 ◽  
Vol 69 (8) ◽  
pp. 350-353
Author(s):  
John L. Morse
Keyword(s):  
Classroom Observation ◽  
Handicapped Child ◽  
Test Results ◽  
Visual Condition ◽  
Inappropriate Behaviors ◽  
Test Conditions ◽  
Visually Handicapped ◽  
First Time

The most common question asked by the psychologist who is faced for the first time with assessing a visually handicapped child are answered. The 15 questions include such areas as the required information concerning visual condition, background of the client, test conditions, the role of the parents, classroom observation, behaviors observed during testing, evaluation of test results, expectations of parents and teachers, and modification of a child's inappropriate behaviors.

Moving to e-bidding

2019 ◽  
Vol 24 (1) ◽  
pp. 2-18 ◽  
Author(s):  
Djoen San Santoso ◽  
Nuttapon Bourpanus
Keyword(s):  
Wilcoxon Test ◽  
Test Results ◽  
Public Infrastructure ◽  
Senior Managers ◽  
Content Type ◽  
Thai Government ◽  
Infrastructure Project ◽  
The Difference ◽  
Public Money ◽  
Mean Differences

Purpose This study aims to examine the influences of shifting the bidding system of Thai public infrastructure projects from e-auction to e-bidding. Design/methodology/approach A questionnaire survey was conducted with owners or senior managers with direct responsibility in deciding the mark-up of 72 small and medium-sized contractor firms. Five senior professionals were interviewed to provide insights into and to strengthen the discussion of the findings. The Wilcoxon test was applied to analyze the difference in the importance of the factors between e-auction and e-bidding. Findings The results revealed a shift in the importance of the factors, from those related to the financial aspects in the e-auction to the situational aspects in the e-bidding. The comparison test results also suggested that the majority of factors become significantly less important in the e-bidding system, with “identity of competitors” and “general expense of the bidding process” having the most apparent mean differences. The interview results supported by data on winning prices and estimations strongly indicated that bid collusions likely exist in the e-auction. By shifting to e-bidding, the data also show that the Thai Government can save public money in its infrastructure project development. Originality/value The study provides an analysis from the perspectives of contractor firms on how e-auction and e-bidding options influence bid mark-up decisions. Many studies have focused on the issues and advantages provided by the e-procurement mainly from the owner (government)’s perspective but how the change influences the contractor’s attitude has been less explored.

scholarly journals The Susceptibility of Meningococci to the Antimicrobial Agents in Moscow 2006 - 2015

2016 ◽  
Vol 15 (3) ◽  
pp. 7-14 ◽  
Author(s):  
M. A. Koroleva ◽  
I. S. Koroleva ◽  
I. M. Zakroeva ◽  
I. M. Gruber
Keyword(s):  
Cerebrospinal Fluid ◽  
Continuous Monitoring ◽  
Antimicrobial Agents ◽  
Meningococcal Infection ◽  
Test Results ◽  
Prognostic Criteria ◽  
Comparison Results ◽  
Moderately Resistant ◽  
First Time

Relevance. One of the prognostic criteria meningococcal infection (MI) epidemic status process is the increasing number of resistant to antibiotics meningococcal strains. Aim of this study was to investigate the dynamics of invasive strains of N. meningitidis susceptibility to antibiotics in Moscow in 2006 - 2015. Materials and methods. Studied 98 strains of N. meningitidis, isolated from blood and cerebrospinal fluid of patients with MI. The study changes of sensitivity N. meningitidis to antibiotics was occured in two periods: first -2006 - 2011 and second - 2012 - 2015. The MIC was determined by E-test. Results. In the present study revealed for the first time the Russian strains of N. meningitidis, moderately resistant to penicillin (5 strains) and resistant to rifampicin (3 strains). Among the studied strains were not found resistant to ceftriaxone, ciprofloxacin, tetracycline and chloramphenicol. Discussion. Comparison results two study periods allowed to reveal the dynamics of increasing the sensitivity of N. meningitidis to antibiotics, which confirms the decline in meningococcal virulence, and as a result, continued interepidemic MI period. Conclusions. Despite the decline antibakterial resistance is required continuous monitoring.

scholarly journals Pelatihan Upaya Kesehatan Kerja Bagi Masyarakat Cipayung Kota Depok

2020 ◽  
Vol 4 (4) ◽  
pp. 605-610
Author(s):  
Azizah Musliha Fitri ◽  
Fajaria Nurcandra ◽  
Terry Yuliana Rahadian Pristya
Keyword(s):  
Test Scores ◽  
Residential Location ◽  
Wilcoxon Test ◽  
P Value ◽  
Test Results ◽  
Average Value ◽  
Post Test ◽  
Level Of Knowledge ◽  
Initial Survey ◽  
The Village

An initial survey that had been done  found that the location of the settlement where residents of cipayung lived was very close to the location of the Cipayung Landfill. The unhealthy environment tended to cause disease and any other health problems, so that efforts needed to be made to overcome this, one effort that could be done is training on health . This counseling activity was attended by 21 residents living in the village of West Bulak which is the closest residential location to the Cipayung landfill site. Wilcoxon test results on the pre-test and post-test scores of participants showed a P- value of 0.001 which means that there were differences in the level of knowledge of the participants after attending counseling. The average value of pre-test and post-test had a difference of 34.5 shows that counseling that has been carried out is able to increase knowledge.

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