The rat hippocampal slice preparation as an in vitro model of ischemia.

Stroke ◽  
1988 ◽  
Vol 19 (4) ◽  
pp. 498-502 ◽  
Author(s):  
W Q Dong ◽  
A Schurr ◽  
K H Reid ◽  
C B Shields ◽  
C A West
Keyword(s):  
In Vitro Model ◽  
Hippocampal Slice ◽  
Slice Preparation ◽  
Vitro Model

Size Does Matter: Generation of Intrinsic Network Rhythms in Thick Mouse Hippocampal Slices

2005 ◽  
Vol 93 (4) ◽  
pp. 2302-2317 ◽  
Author(s):  
Chiping Wu ◽  
Wah Ping Luk ◽  
Jesse Gillis ◽  
Frances Skinner ◽  
Liang Zhang
Keyword(s):  
In Vitro Model ◽  
Adult Mouse ◽  
Hippocampal Slices ◽  
Network Connectivity ◽  
Slice Preparation ◽  
Fundamental Properties ◽  
Mouse Hippocampus ◽  
Hippocampal Network ◽  
Vitro Model

Rodent hippocampal slices of ≤0.5 mm thickness have been widely used as a convenient in vitro model since the 1970s. However, spontaneous population rhythmic activities do not consistently occur in this preparation due to limited network connectivity. To overcome this limitation, we develop a novel slice preparation of 1 mm thickness from adult mouse hippocampus by separating dentate gyrus from CA3/CA1 areas but preserving dentate–CA3-CA1 connectivity. While superfused in vitro at 32 or 37°C, the thick slice exhibits robust spontaneous network rhythms of 1–4 Hz that originate from the CA3 area. Via assessing tissue O2, K+, pH, synaptic, and single-cell activities of superfused thick slices, we verify that these spontaneous rhythms are not a consequence of hypoxia and nonspecific experimental artifacts. We suggest that the thick slice contains a unitary circuitry sufficient to generate intrinsic hippocampal network rhythms and this preparation is suitable for exploring the fundamental properties and plasticity of a functionally defined hippocampal “lamella” in vitro.

The midbrain slice preparation. An in vitro model to select potential anti-parkinsonian drugs?

2008 ◽  
Vol 14 ◽  
pp. S150-S153 ◽  
Author(s):  
Ezia Guatteo ◽  
Maria Letizia Cucchiaroni ◽  
Luca Sebastianelli ◽  
Giorgio Bernardi ◽  
Nicola Biagio Mercuri
Keyword(s):  
In Vitro Model ◽  
Slice Preparation ◽  
Vitro Model

Propofol Reduces Neuronal Transmission Damage and Attenuates the Changes in Calcium, Potassium, and Sodium during Hyperthermic Anoxia in the Rat Hippocampal Slice

1995 ◽  
Vol 83 (6) ◽  
pp. 1254-1265 ◽  
Author(s):  
Pedro Amorim ◽  
Geoffrey Chambers ◽  
James Cottrell ◽  
Ira S. Kass
Keyword(s):  
Blood Flow ◽  
In Vitro Model ◽  
Hippocampal Slice ◽  
In Vivo Studies ◽  
Ischemic Brain ◽  
Cerebral Metabolic Rate ◽  
Neuronal Transmission ◽  
Vitro Model

Abstract Background Propofol reduces cerebral blood flow, cerebral metabolic rate for oxygen, and intracranial pressure and is being increasingly used in neuroanesthesia. In vivo studies have yielded conflicting results on its ability to protect against ischemic brain damage. In the current study, an in vitro model was used to examine the mechanism of propofol's action on anoxic neuronal transmission damage.

A simple in vitro model of ischemia based on hippocampal slice cultures and propidium iodide fluorescence

1999 ◽  
Vol 4 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Jon Henrik Laake ◽  
Finn-Mogens Haug ◽  
Tadeusz Wieloch ◽  
Ole Petter Ottersen
Keyword(s):  
Propidium Iodide ◽  
In Vitro Model ◽  
Hippocampal Slice ◽  
Hippocampal Slice Cultures ◽  
Vitro Model

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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