Endothelin Production in Cultured Mesangial Cells of Spontaneously Hypertensive Rats

Hypertension ◽  
1995 ◽  
Vol 25 (4) ◽  
pp. 1-6
Author(s):  
Miwako Ikeda ◽  
Masakazu Kohno ◽  
Tadanao Takeda
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Mesangial Cells ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Characterization of signaling pathways of Angiotensin I‐Converting Enzyme in mesangial cells of spontaneously hypertensive rats (SHR)

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Rosana Inácio Reis ◽  
Lucas Tabajara Parreiras‐e‐Silva ◽  
Christiane Becari ◽  
Maria Claudina Andrade ◽  
Maria Cristina Oliveira Salgado ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Spontaneously Hypertensive Rats ◽  
Mesangial Cells ◽  
Converting Enzyme ◽  
Hypertensive Rats ◽  
Angiotensin I ◽  
Spontaneously Hypertensive ◽  
Angiotensin I Converting Enzyme

scholarly journals Insulin nonattenuation of vasoactive agent-induced responses in mesangial cells from spontaneously hypertensive rats

1995 ◽  
Vol 47 (3) ◽  
pp. 891-898 ◽  
Author(s):  
Yoji Inishi ◽  
Toshihiro Okuda ◽  
Toshio Arakawa ◽  
Chikako Yasuda ◽  
Mamiko Ohara ◽  
...  
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Mesangial Cells ◽  
Induced Responses ◽  
Hypertensive Rats ◽  
Vasoactive Agent ◽  
Spontaneously Hypertensive

scholarly journals Higher Bumetanide Sensitive Na-K-Cl Cotransport in Cultured Mesangial Cells from Spontaneously Hypertensive Rats (SHR)

1991 ◽  
Vol 32 (4) ◽  
pp. 558-558
Author(s):  
Satoru Kuriyama ◽  
Koji Nakamura ◽  
Yoshihiko Kaguchi ◽  
Takao Hashimoto ◽  
Osamu Sakai
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Mesangial Cells ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Modification of mesangial cell function by chloride is attenuated in spontaneously hypertensive rats

1994 ◽  
Vol 266 (4) ◽  
pp. F586-F591 ◽  
Author(s):  
T. Okuda ◽  
Y. Inishi ◽  
T. Arakawa ◽  
M. Ohara ◽  
K. Kurokawa
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Cell Function ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Pge2 Production ◽  
Ang Ii ◽  
Hypertensive Rats ◽  
Cell Contraction ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto

The effects of extracellular Cl- concentration ([Cl-]o) on cultured mesangial cells from spontaneously hypertensive rats (SHR) were examined. Angiotensin II (ANG II)- and vasopressin (VP)-induced cell contraction and Ca2+ transients of SHR mesangial cells were unaffected when the cells were preincubated with 10 mM [Cl-]o, while obvious suppression of the responses to these agents was observed in Wistar-Kyoto (WKY) mesangial cells. Enhanced prostaglandin E2 (PGE2) production was elicited by a decrease in [Cl-]o in WKY mesangial cells. In contrast, PGE2 synthesis by SHR mesangial cells was not enhanced by low [Cl-]o. However, ANG II-stimulated PGE2 production and the attenuation of ANG II-induced cell contraction and Ca2+ transients by the addition of exogenous PGE2 were present equally in both WKY and SHR mesangial cells. Based on these findings, we conclude that the absence of modification of mesangial cell function by [Cl-]o in SHR is due to the inability of low [Cl-]o to enhance PGE2 production. Insensitivity of SHR mesangial cells to changes in [Cl-]o might underlie the dysregulation of renal function in SHR.

Role of complement 3a in the growth of mesangial cells from stroke-prone spontaneously hypertensive rats

2013 ◽  
Vol 36 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Kazuya Ikeda ◽  
Noboru Fukuda ◽  
Takahiro Ueno ◽  
Morito Endo ◽  
Naohiko Kobayashi ◽  
...  
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Mesangial Cells ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

scholarly journals Expression and localization of N-domain ANG I-converting enzymes in mesangial cells in culture from spontaneously hypertensive rats

2006 ◽  
Vol 290 (2) ◽  
pp. F364-F375 ◽  
Author(s):  
Maria Claudina Camargo de Andrade ◽  
Giovana Seno Di Marco ◽  
Vicente de Paulo Castro Teixeira ◽  
Renato Arruda Mortara ◽  
Regiane Angélica Sabatini ◽  
...  
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Wistar Rats ◽  
Mesangial Cells ◽  
Gel Filtration ◽  
Cell Fractionation ◽  
Ang Ii ◽  
Cell Nuclei ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
New Genes

The angiotensin-converting enzyme (ACE) profile in urine of hypertensive patients and spontaneously hypertensive rats (SHR; 90- and 65-kDa N-domain ACEs) is different from that of healthy subjects and Wistar rats (190 and 65 kDa). In addition, four ACE isoforms were purified from mesangial cells (MC) of Wistar rats in the intracellular compartment (130 and 68 kDa) and as secreted forms (130 and 60 kDa). We decided to characterize ACE forms from SHR MC in culture. Analysis of the ACE gene showed that SHR MC are able to express ACE mRNA. The concentrated medium and cell homogenate were separately purified by gel filtration and then subjected to lisinopril-Sepharose chromatography. The molecular masses of purified enzymes, 90 kDa for ACEm1A and 65 kDa for ACEm2A (secreted enzymes) and 90 kDa for ACEInth1A and 65 kDa for ACEInth2A (intracellular), were different from those of Wistar MC. The purified enzymes are Cl− dependent, inhibited by enalaprilat and captopril, and able to hydrolyze AcSDKP. Immunofluorescence and cell fractionation followed by Western blotting showed predominant immunoreaction of the 9B9 antiserum for N-domain ACE in the nuclei. The N-domain ACE was localized in the glomerulus from Wistar rats and SHR. ANG II and ANG-(1–7) were localized in the cell cytoplasm and nuclei. The 90-kDa N-domain ACE, described recently as a possible genetic marker of hypertension, was found inside the cell nuclei of SHR MC colocalized with ANG II and ANG-(1–7). The presence of ANG II in the cell nuclei could suggest an important role for this peptide in the transcription of new genes.

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