scholarly journals Regulation of contraction and relaxation in arterial smooth muscle.

Hypertension ◽  
1992 ◽  
Vol 20 (2) ◽  
pp. 129-137 ◽  
Author(s):  
C M Rembold
Keyword(s):  
Smooth Muscle ◽  
Arterial Smooth Muscle ◽  
Regulation Of Contraction ◽  
Contraction And Relaxation

scholarly journals Phosphorylated HSP20 modulates the association of thin-filament binding proteins: caldesmon with tropomyosin in colonic smooth muscle

2010 ◽  
Vol 299 (5) ◽  
pp. G1164-G1176 ◽  
Author(s):  
Sita Somara ◽  
Robert R. Gilmont ◽  
Saranyaraajan Varadarajan ◽  
Khalil N. Bitar
Keyword(s):  
Smooth Muscle ◽  
Thin Filament ◽  
Muscle Activation ◽  
Molecular Signaling ◽  
Phosphorylation State ◽  
Filament Dynamics ◽  
Physiological Relevance ◽  
Regulation Of Contraction ◽  
Shock Proteins ◽  
Contraction And Relaxation

Small heat shock proteins HSP27 and HSP20 have been implicated in regulation of contraction and relaxation in smooth muscle. Activation of PKC-α promotes contraction by phosphorylation of HSP27 whereas activation of PKA promotes relaxation by phosphorylation of HSP20 in colonic smooth muscle cells (CSMC). We propose that the balance between the phosphorylation states of HSP27 and HSP20 represents a molecular signaling switch for contraction and relaxation. This molecular signaling switch acts downstream on a molecular mechanical switch [tropomyosin (TM)] regulating thin-filament dynamics. We have examined the role of phosphorylation state(s) of HSP20 on HSP27-mediated thin-filament regulation in CSMC. CSMC were transfected with different HSP20 phosphomutants. These transfections had no effect on the integrity of actin cytoskeleton. Cells transfected with 16D-HSP20 (phosphomimic) exhibited inhibition of acetylcholine (ACh)-induced contraction whereas cells transfected with 16A-HSP20 (nonphosphorylatable) had no effect on ACh-induced contraction. CSMC transfected with 16D-HSP20 cDNA showed significant decreases in 1) phosphorylation of HSP27 (ser78); 2) phosphorylation of PKC-α (ser657); 3) phosphorylation of TM and CaD (ser789); 4) ACh-induced phosphorylation of myosin light chain; 5) ACh-induced association of TM with HSP27; and 6) ACh-induced dissociation of TM from caldesmon (CaD). We thus propose the crucial physiological relevance of molecular signaling switch (phosphorylation state of HSP27 and HSP20), which dictates 1) the phosphorylation states of TM and CaD and 2) their dissociations from each other.

scholarly journals Contractility of the epididymal duct - function, regulation and potential drug effects

Reproduction ◽  
2018 ◽  
Author(s):  
Vera Elfgen ◽  
Andrea Mietens ◽  
Marco Mewe ◽  
Thorben Hau ◽  
Ralf Middendorff
Keyword(s):  
Smooth Muscle ◽  
Clinical Practice ◽  
Side Effects ◽  
Male Fertility ◽  
Vas Deferens ◽  
Drug Effects ◽  
Potential Drug ◽  
Regulation Of Contraction ◽  
Contraction And Relaxation ◽  
Epididymal Duct

During their transit through the epididymis, spermatozoa mature and acquire motility and fertilizing capacity. The smooth muscle cells (SMCs) of the epididymal duct are thought to be responsible for the adequate transport of spermatozoa. Thus, precise regulation of SMC function also represents a prerequisite for sperm maturation thereby contributing to male fertility. In this review we would like to highlight various aspects of epididymal SMC function and discuss several angles with respect to regulation of contraction and relaxation. Different to the vas deferens, where disturbed SMC pathways resulting in male infertility could be defined, comparable information is missing in the epididymis. We therefore include some vas deferens data which could also be useful for a better understanding of epididymal SMC function. Furthermore, we would like to draw attention to drugs used in clinical practice and their potential (side) effects on contractions in the epididymis.

Phenotypic Heterogeneity of Rat Arterial Smooth Muscle Cell Clones

1996 ◽  
Vol 16 (6) ◽  
pp. 815-820 ◽  
Author(s):  
Marie-Luce Bochaton-Piallat ◽  
Patricia Ropraz ◽  
Françoise Gabbiani ◽  
Giulio Gabbiani
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Phenotypic Heterogeneity ◽  
Arterial Smooth Muscle Cell ◽  
Arterial Smooth Muscle ◽  
Cell Clones

scholarly journals Effects of vasopressin on ATP-sensitive and Ca2+-activated K+ channels of coronary arterial smooth muscle cells

1992 ◽  
Vol 58 ◽  
pp. 339
Author(s):  
Tetsuzo Wakatsuki ◽  
Yutaka Nakaya ◽  
Yukiko Miyoshi ◽  
Zeng Xiao-Rong ◽  
Masahiro Nomura ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Arterial Smooth Muscle ◽  
K Channels ◽  
Arterial Smooth Muscle Cells
Sign in / Sign up

Export Citation Format

Share Document