Real-Time Assessment of Guided Bone Regeneration in Standardized Calvarial Defects Using a Combination of Bone Graft and Platelet-Derived Growth Factor With and Without Collagen Membrane: An In Vivo Microcomputed Tomographic and Histologic Experiment in Rats

2016 ◽  
Vol 36 ◽  
pp. s173-s186 ◽  
Author(s):  
Abdulaziz Al-Rasheed ◽  
Fatemah Al-Ahmari ◽  
Sundar Ramalingam ◽  
Nasser Nooh ◽  
Cun-Yu Wang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Real Time ◽  
Guided Bone Regeneration ◽  
Platelet Derived Growth Factor ◽  
Collagen Membrane ◽  
Calvarial Defects ◽  
Time Assessment

scholarly journals Comparative In Vivo Analysis of the Integration Behavior and Immune Response of Collagen-Based Dental Barrier Membranes for Guided Bone Regeneration (GBR)

Membranes ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 712
Author(s):  
Milena Radenković ◽  
Said Alkildani ◽  
Ignacio Stoewe ◽  
James Bielenstein ◽  
Bernd Sundag ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bone Regeneration ◽  
Barrier Function ◽  
Guided Bone Regeneration ◽  
Collagen Membrane ◽  
Cellular Infiltration ◽  
Barrier Membranes ◽  
Tissue Integration ◽  
Tissue Reactions

Collagen-based resorbable barrier membranes have been increasingly utilized for Guided Bone Regeneration (GBR), as an alternative to non-resorbable synthetic membranes that require a second surgical intervention for removal. One of the most important characteristics of a resorbable barrier membrane is its mechanical integrity that is required for space maintenance and its tissue integration that plays a crucial role in wound healing and bone augmentation. This study compares a commercially available porcine-derived sugar-crosslinked collagen membrane with two non-crosslinked collagen barrier membranes. The material analysis provides an insight into the influence of manufacturing on the microstructure. In vivo subcutaneous implantation model provides further information on the host tissue reaction of the barrier membranes, as well as their tissue integration patterns that involve cellular infiltration, vascularization, and degradation. The obtained histochemical and immunohistochemical results over three time points (10, 30, and 60 days) showed that the tissue response to the sugar crosslinked collagen membrane involves inflammatory macrophages in a comparable manner to the macrophages observed in the surrounding tissue of the control collagen-based membranes, which were proven as biocompatible. The tissue reactions to the barrier membranes were additionally compared to wounds from a sham operation. Results suggest wound healing properties of all the investigated barrier membranes. However, the sugar-crosslinked membrane lacked in cellular infiltration and transmembraneous vascularization, providing an exclusive barrier function in GBR. Moreover, this membrane maintained a similar swelling ratio over examined timepoints, which suggests a very slow degradation pattern and supports its barrier function. Based on the study results, which showed biocompatibility of the sugar crosslinked membrane and its stability up to 60 days post-implantation, it can be concluded that this membrane may be suitable for application in GBR as a biomaterial with exclusive barrier functionality, similar to non-resorbable options.

Guided Bone Regeneration of Femoral Segmental Defects using Equine Bone Graft: An In-Vivo Micro-Computed Tomographic Study in Rats

2018 ◽  
Vol 32 (5) ◽  
pp. 456-466 ◽  
Author(s):  
Mohammed Awadh Binsalah ◽  
Sundar Ramalingam ◽  
Mohammed Alkindi ◽  
Nasser Nooh ◽  
Khalid Al-Hezaimi
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Computed Tomographic ◽  
Equine Bone

scholarly journals In Vitro and In Vivo Study of a Novel Porcine Collagen Membrane for Guided Bone Regeneration

Materials ◽  
2016 ◽  
Vol 9 (11) ◽  
pp. 949 ◽  
Author(s):  
Eisner Salamanca ◽  
Chi-Yang Tsai ◽  
Yu-Hwa Pan ◽  
Yu-Te Lin ◽  
Haw-Ming Huang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
In Vivo Study ◽  
Collagen Membrane ◽  
Porcine Collagen

scholarly journals In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes

Membranes ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 423
Author(s):  
Abdu Mansur Dacache Neto ◽  
Suelen Cristina Sartoretto ◽  
Isabelle Martins Duarte ◽  
Rodrigo Figueiredo de Brito Resende ◽  
Adriana Terezinha Neves Novellino Alves ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Guided Bone Regeneration ◽  
Tissue Reaction ◽  
Clinical Indication ◽  
Collagen Membrane ◽  
Tissue Reactions ◽  
Porcine Collagen

Mechanical barriers prevent the invasion of the surrounding soft tissues within the bone defects. This concept is known as Guided Bone Regeneration (GBR). The knowledge about the local tissue reaction and the time of degradation of absorbable membranes favors the correct clinical indication. This study aimed to evaluate the biocompatibility and biodegradation of a bovine collagen membrane (Lyostypt®, São Gonçalo, Brazil) and compare it to a porcine collagen membrane (Bio-Gide®) implanted in the subcutaneous tissue of mice, following ISO 10993-6:2016. Thirty Balb-C mice were randomly divided into three experimental groups, LT (Lyostypt®), BG (Bio-Gide®), and Sham (without implantation), and subdivided according to the experimental periods (7, 21, and 63 days). The BG was considered non-irritant at seven days and slight and moderate irritant at 21 and 63 days, respectively. The LT presented a small irritant reaction at seven days, a mild reaction after 21, and a reduction in the inflammatory response at 63 days. The biodegradation of the LT occurred more rapidly compared to the BG after 63 days. This study concluded that both membranes were considered biocompatible since their tissue reactions were compatible with the physiological inflammatory process; however, the Bio-Gide® was less degraded during the experimental periods, favoring the guided bone regeneration process.

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