Molecular Assessment of Osseointegration In Vitro: A Review of Current Literature

2014 ◽  
Vol 29 (2) ◽  
pp. e171-e199 ◽  
Author(s):  
Ghadeer Thalji ◽  
Lyndon F. Cooper
Keyword(s):  
Current Literature ◽  
Molecular Assessment

scholarly journals GENE EXPRESSION DURING INDIRECT SOMATIC EMBRYOGENESIS OF PLANTS

2004 ◽  
Vol 42 (2) ◽  
Author(s):  
Tammy Estabrooks ◽  
Zhongmin Dong
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Embryo Development ◽  
Current Literature ◽  
Molecular Mechanisms ◽  
Indirect Somatic Embryogenesis ◽  
Experimental Tool ◽  
Plant Embryo ◽  
Plant Embryo Development

Somatic embryogenesis is the process by which somatic cells are induced into an embryogenic state, followed by differentiation into embryos. Somatic embryogenesis, in addition to being a method of propagation, can serve as an experimental tool for research into plant embryo development. This is a review of the current literature on in vitro plant somatic embryogenesis and the molecular advances made to identify genes expressed during the various stages of this process. Some factors hindering the elucidation of the molecular mechanisms underlying somatic embryogenesis are discussed.L’embryogenèse somatique est le processus par lequel les cellules somatiques passent à l’état embryogène et se différencient en embryons. En plus de constituer une méthode de propagation, elle peut servir d’outil expérimental de recherche pour développer des embryons de plantes. Le présent document est une revue de la documentation sur l’embryogenèse somatique végétale in vitro et sur les progrès réalisés à l’échelle moléculaire pour identifier les gènes exprimés au cours des divers stades du processus. On examine aussi certains facteurs qui rendent difficile l’élucidation des mécanismes moléculaires de l’embryogenèse somatique.

NF-кB inhibition by DHMEQ: in vitro antiproliferative effects on pilocytic astrocytoma and concise review of the current literature

2020 ◽  
Vol 36 (11) ◽  
pp. 2675-2684
Author(s):  
M. F. Candido ◽  
G. C. Baldissera ◽  
M. Medeiros ◽  
K. Umezawa ◽  
María Sol Brassesco
Keyword(s):  
Current Literature ◽  
Pilocytic Astrocytoma ◽  
Antiproliferative Effects ◽  
Concise Review

scholarly journals Mebendazole as a Candidate for Drug Repurposing in Oncology: An Extensive Review of Current Literature

Cancers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1284 ◽  
Author(s):  
Guerini ◽  
Triggiani ◽  
Maddalo ◽  
Bonù ◽  
Frassine ◽  
...  
Keyword(s):  
Current Literature ◽  
Single Agent ◽  
Drug Repurposing ◽  
Chemotherapeutic Agents ◽  
Metastatic Spread ◽  
Multi Drug Resistance ◽  
Wide Range ◽  
Disease Site

Anticancer treatment efficacy is limited by the development of refractory tumor cells characterized by increased expression and activity of mechanisms promoting survival, proliferation, and metastatic spread. The present review summarizes the current literature regarding the use of the anthelmintic mebendazole (MBZ) as a repurposed drug in oncology with a focus on cells resistant to approved therapies, including so called “cancer stem cells”. Mebendazole meets many of the characteristics desirable for a repurposed drug: good and proven toxicity profile, pharmacokinetics allowing to reach therapeutic concentrations at disease site, ease of administration and low price. Several in vitro studies suggest that MBZ inhibits a wide range of factors involved in tumor progression such as tubulin polymerization, angiogenesis, pro-survival pathways, matrix metalloproteinases, and multi-drug resistance protein transporters. Mebendazole not only exhibits direct cytotoxic activity, but also synergizes with ionizing radiations and different chemotherapeutic agents and stimulates antitumoral immune response. In vivo, MBZ treatment as a single agent or in combination with chemotherapy led to the reduction or complete arrest of tumor growth, marked decrease of metastatic spread, and improvement of survival. Further investigations are warranted to confirm the clinical anti-neoplastic activity of MBZ and its safety in combination with other drugs in a clinical setting.

In Vitro and Molecular Assessment for Lead Tolerance in Aloevera L. Callus

2019 ◽  
Vol 10 (10) ◽  
pp. 1030
Author(s):  
Rana A. Hameed Al-Roomi ◽  
Hashim K. Mohammed Al-Oubaidi ◽  
Rana Hadi Hameed
Keyword(s):  
Lead Tolerance ◽  
Molecular Assessment

scholarly journals Emerging Threats for Human Health in Poland: Pathogenic Isolates from Drug ResistantAcanthamoebaKeratitis Monitored in terms of TheirIn VitroDynamics and Temperature Adaptability

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Lidia Chomicz ◽  
David Bruce Conn ◽  
Marcin Padzik ◽  
Jacek P. Szaflik ◽  
Julia Walochnik ◽  
...  
Keyword(s):  
Human Health ◽  
Ocular Infection ◽  
Pathogenic Potential ◽  
The Past ◽  
Unsuccessful Treatment ◽  
Adaptive Capability ◽  
Causative Agents ◽  
Molecular Assessment ◽  
Temperature Adaptability

Amphizoic amoebae generate a serious human health threat due to their pathogenic potential as facultative parasites, causative agents of vision-threateningAcanthamoebakeratitis (AK). Recently, AK incidences have been reported with increasing frequency worldwide, particularly in contact lens wearers. In our study, severe cases of AK in Poland and respective pathogenic isolates were assessed at clinical, morphological, and molecular levels. Misdiagnoses and the unsuccessful treatment in other ophthalmic units delayed suitable therapy, and resistance to applied chemicals resulted in severe courses and treatment difficulties. Molecular assessment indicated that all sequenced pathogenic corneal isolates deriving from Polish patients with AK examined by us showed 98–100% homology withAcanthamoebagenotype T4, the most prevalent genotype in this human ocular infection worldwide.In vitroassays revealed that the pathogenic strains are able to grow at elevated temperature and have a wide adaptive capability. This study is our subsequentin vitroinvestigation on pathogenicAcanthamoebastrains of AK originating from Polish patients. Further investigations designed to foster a better understanding of the factors leading to an increase of AK observed in the past years in Poland may help to prevent or at least better cope with future cases.

scholarly journals A New Aspect for In vitro Propagation of Jerusalem Artichoke and Molecular Assessment Using RAPD, ISSR and SCoT Marker Techniques

2020 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Neama Abdalla ◽  
Mohamed Ragab ◽  
Salah El-Miniawy ◽  
Nermeen Arafa ◽  
Hussein Taha
Keyword(s):  
In Vitro Propagation ◽  
Jerusalem Artichoke ◽  
Scot Marker ◽  
Molecular Assessment

scholarly journals Polyphenols vs. Coronaviruses: How Far Has Research Moved Forward?

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4103
Author(s):  
Simona Piccolella ◽  
Giuseppina Crescente ◽  
Shadab Faramarzi ◽  
Marialuisa Formato ◽  
Maria Tommasina Pecoraro ◽  
...  
Keyword(s):  
Experimental Data ◽  
Current Literature ◽  
Scientific Knowledge ◽  
Driving Force ◽  
Antiviral Role

The epidemic, caused by SARS-CoV-2 at the beginning of 2020, led us to a serious change in our lifestyle that for about three months has confined us to our homes, far from our laboratory routine. In this period, the belief that the work of a researcher should never stop has been the driving force in writing the present paper. It aims at reviewing the recent scientific knowledge about in vitro experimental data that focused on the antiviral role of phenols and polyphenols against different species of coronaviruses (CoVs), pointing up the viral targets potentially involved. In the current literature scenario, the papain-like and the 3-chymotrypsin-like proteases seem to be the most deeply investigated and a number of isolated natural (poly)phenols has been screened for their efficacy.

scholarly journals Computing Osmotic Permeabilities of Aquaporins AQP4, AQP5, and GlpF from Near-Equilibrium Simulations

2017 ◽  
Author(s):  
Thierry Oscar Wambo ◽  
Roberto Alexis Rodriguez ◽  
Liao Y Chen
Keyword(s):  
Current Literature ◽  
Large Scale ◽  
Single Channel ◽  
Force Fields ◽  
Activation Energies ◽  
Water Current ◽  
Single File ◽  
Large Scale Simulations ◽  
Single File Diffusion

Measuring or computing the single-channel permeability of aquaporins/aquaglyceroporins (AQPs) has long been a challenge. The measured values scatter over an order of magnitude but the corresponding Arrhenius activation energies converge in the current literature. Osmotic flux through an AQP was simulated as water current forced through the channel by kilobar hydraulic pressure or theoretically approximated as single-file diffusion. In this paper, we report large scale simulations of osmotic current under sub M gradient through three water channels (the water-specific AQP4 and AQP5 along with aquaglyceroporin GlpF) using the mature particle mesh Ewald technique (PME) for which the established force fields have been optimized with known accuracy. These simulations were implemented with hybrid periodic boundary conditions devised to avoid the artifactitious mixing across the membrane in a regular PME simulation. The computed single-channel permeabilities are in agreement with recently refined experiments on GlpF. The Arrhenius activation energies extracted from our simulations for all the three AQPs agree with the in vitro measurements. The single-file diffusion approximations from our large-scale simulations are consistent with the current literature on smaller systems. From these unambiguous agreements among the in vitro and in silico studies, we observe the quantitative accuracy of the all-atom force fields of the current literature for water-channel biology. We also observe that AQP4, that is particularly rich in the central nervous system, is more efficient in water conduction and more temperature-sensitive than other water-specific channel proteins.

Sign in / Sign up

Export Citation Format

Share Document