Xenograft Impregnated with Bone Marrow Mononuclear Fraction for Appositional Bone Regeneration in Rabbit Calvaria: A Clinical and Histomorphometric Study

2014 ◽  
Vol 29 (4) ◽  
pp. 962-968 ◽  
Author(s):  
Rafael Oliveira ◽  
André Pelegrine ◽  
Antonio Aloise ◽  
Lydia Ferreira
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Histomorphometric Study ◽  
Appositional Bone

scholarly journals Sinusoidal electromagnetic fields accelerate bone regeneration by boosting the multifunctionality of bone marrow mesenchymal stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weigang Li ◽  
Wenbin Liu ◽  
Wei Wang ◽  
Jiachen Wang ◽  
Tian Ma ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Electromagnetic Fields ◽  
Bone Defects ◽  
Cell Scaffolds ◽  
Marrow Mesenchymal Stem Cells ◽  
Calvarial Defects

Abstract Background The repair of critical-sized bone defects is always a challenging problem. Electromagnetic fields (EMFs), used as a physiotherapy for bone defects, have been suspected to cause potential hazards to human health due to the long-term exposure. To optimize the application of EMF while avoiding its adverse effects, a combination of EMF and tissue engineering techniques is critical. Furthermore, a deeper understanding of the mechanism of action of EMF will lead to better applications in the future. Methods In this research, bone marrow mesenchymal stem cells (BMSCs) seeded on 3D-printed scaffolds were treated with sinusoidal EMFs in vitro. Then, 5.5 mm critical-sized calvarial defects were created in rats, and the cell scaffolds were implanted into the defects. In addition, the molecular and cellular mechanisms by which EMFs regulate BMSCs were explored with various approaches to gain deeper insight into the effects of EMFs. Results The cell scaffolds treated with EMF successfully accelerated the repair of critical-sized calvarial defects. Further studies revealed that EMF could not directly induce the differentiation of BMSCs but improved the sensitivity of BMSCs to BMP signals by upregulating the quantity of specific BMP (bone morphogenetic protein) receptors. Once these receptors receive BMP signals from the surrounding milieu, a cascade of reactions is initiated to promote osteogenic differentiation via the BMP/Smad signalling pathway. Moreover, the cytokines secreted by BMSCs treated with EMF can better facilitate angiogenesis and osteoimmunomodulation which play fundamental roles in bone regeneration. Conclusion In summary, EMF can promote the osteogenic potential of BMSCs and enhance the paracrine function of BMSCs to facilitate bone regeneration. These findings highlight the profound impact of EMF on tissue engineering and provide a new strategy for the clinical treatment of bone defects.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells

Nanoscale ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 7284-7300 ◽  
Author(s):  
Xiangfeng Li ◽  
Minjun Liu ◽  
Fuying Chen ◽  
Yuyi Wang ◽  
Menglu Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Natural Bone ◽  
Morphogenetic Protein ◽  
Bone Apatite

Biomimicking the nanostructure of natural bone apatite to enhance the bioactivity of hydroxyapatite (HA) biomaterials is an eternal topic in the bone regeneration field.

scholarly journals Ex vivo gene therapy in autologous bone marrow stromal stem cells for tissue-engineered maxillofacial bone regeneration

Gene Therapy ◽  
2003 ◽  
Vol 10 (24) ◽  
pp. 2013-2019 ◽  
Author(s):  
S C-N Chang ◽  
H L Chuang ◽  
Y R Chen ◽  
J K Chen ◽  
H-Y Chung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Ex Vivo ◽  
Autologous Bone Marrow ◽  
Ex Vivo Gene Therapy ◽  
Autologous Bone ◽  
Stromal Stem Cells

Facilitating Osteogenesis of Chitosan with Autogenous Bone Marrow (experimental Study on Rabbits)

2020 ◽  
Vol 23 (2) ◽  
pp. 36-40
Author(s):  
Hawar Asaad Zebari ◽  
Hareth H. Kaskos
Keyword(s):  
Bone Marrow ◽  
Bone Mass ◽  
Bone Regeneration ◽  
Bone Matrix ◽  
Density Measurement ◽  
Autogenous Bone ◽  
Bone Density Measurement ◽  
Matrix Mineralization ◽  
Significant Difference ◽  
Measurement Results

Bone quality is the result of a complex relationship between the intrinsic properties of the materials that comprise the bone matrix mineralization, bone mass and the spatial distribution of the bone mass. Chitosan has been shown to be suitable bone replacement material. To evaluate the accelerating effect of chitosan on the bone regeneration process and assessing by CT Scan were conduct this study. Several important biological effect of chitosan has been characterized, these are high osteoinductivity, osteointegrability and gradual biodegrability that make it a good candidate for bone regeneration. Materials and Methods: 20 rabbits of both sex were enrolled in this study, two monocortical defects were created on Mandible, one considered as control and the other implanted with chitosan, other two monocortical defects were created on Tibia on the same animal. Post-operative follow up date 7,14,21and 28 Days. C.T. scan was used as parameter for bone density measurement. Results: showed that non- significant difference at Day7 and14 in Mandible and significant at Day21 and 28 compared to control, While non-significant at Day 7 in Tibia and significant at 14 and 21 post-operatively with highly significant at Day28 compared to control. Conclusion: Chitosan has ability to osteogenesis when it is used alone and the process of osteogenesis was facilitating when it is mixed with Bone marrow.

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