Effects of Anodized Titanium with Arg-Gly-Asp (RGD) Peptide Immobilized Via Chemical Grafting or Physical Adsorption on Bone Cell Adhesion and Differentiation

2013 ◽  
Vol 28 (4) ◽  
pp. 963-972 ◽  
Author(s):  
Jae-Jun Ryu ◽  
Kyeongsoon Park ◽  
Hyo-Sop Kim ◽  
Chang-Mo Jeong ◽  
Jung-Bo Huh
Keyword(s):  
Cell Adhesion ◽  
Physical Adsorption ◽  
Bone Cell ◽  
Rgd Peptide ◽  
Chemical Grafting ◽  
Anodized Titanium

Histatin‐1 is a novel osteogenic factor that promotes bone cell adhesion, migration, and differentiation

2021 ◽  
Author(s):  
Pedro Torres ◽  
Nadia Hernández ◽  
Carlos Mateluna ◽  
Patricio Silva ◽  
Montserrat Reyes ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Bone Cell ◽  
Osteogenic Factor

scholarly journals Receptor functions for the integrin VLA-3: fibronectin, collagen, and laminin binding are differentially influenced by Arg-Gly-Asp peptide and by divalent cations.

1991 ◽  
Vol 112 (1) ◽  
pp. 169-181 ◽  
Author(s):  
M J Elices ◽  
L A Urry ◽  
M E Hemler
Keyword(s):  
Cell Adhesion ◽  
Divalent Cations ◽  
Small Cell Lung Carcinoma ◽  
Rgd Peptide ◽  
Dependent Manner ◽  
Cell Lung Carcinoma ◽  
Ligand Affinity ◽  
Rgd Site ◽  
Inhibition Studies ◽  
Laminin Binding

The capability of the integrin VLA-3 to function as a receptor for collagen (Coll), laminin (Lm), and fibronectin (Fn) was addressed using both whole cell adhesion assays and ligand affinity columns. Analysis of VLA-3-mediated cell adhesion was facilitated by the use of a small cell lung carcinoma line (NCI-H69), which expresses VLA-3 but few other integrins. While VLA-3 interaction with Fn was often low or undetectable in cells having both VLA-3 and VLA-5, NCI-H69 cells readily attached to Fn in a VLA-3-dependent manner. Both Arg-Gly-Asp (RGD) peptide inhibition studies, and Fn fragment affinity columns suggested that VLA-3, like VLA-5, may bind to the RGD site in human Fn. However, unlike Fn, both Coll and Lm supported VLA-3-mediated adhesion that was not inhibited by RGD peptide, and was totally unaffected by the presence of VLA-5. In addition, VLA-3-mediated binding to Fn was low in the presence of Ca++, but was increased 6.6-fold with Mg++, and 30-fold in the presence of Mn++. In contrast, binding to Coll was increased only 1.2-fold with Mg++, and 1.7-fold in Mn++, as compared to the level seen with Ca++. Together, these experiments indicate that VLA-3 can bind Coll, Lm, and Fn, and also show that (a) VLA-3 can recognize both RGD-dependent and RGD-independent ligands, and (b) different VLA-3 ligands have distinctly dissimilar divalent cation sensitivities.

Buried, Covalently Attached RGD Peptide Motifs in Poly(methacrylic acid) Brush Layers: The Effect of Brush Structure on Cell Adhesion

Langmuir ◽  
2008 ◽  
Vol 24 (19) ◽  
pp. 10996-11002 ◽  
Author(s):  
Melba Navarro ◽  
Edmondo M. Benetti ◽  
Szczepan Zapotoczny ◽  
Josep A. Planell ◽  
G. Julius Vancso
Keyword(s):  
Cell Adhesion ◽  
Methacrylic Acid ◽  
Rgd Peptide ◽  
Peptide Motifs

RAFT polymerization of a RGD peptide-based methacrylamide monomer for cell adhesion

2018 ◽  
Vol 9 (14) ◽  
pp. 1780-1786 ◽  
Author(s):  
Chao Chen ◽  
San H. Thang
Keyword(s):  
Cell Adhesion ◽  
Raft Polymerization ◽  
Rgd Peptide ◽  
Robust Method ◽  
Support Cell

The present study provides a robust method for the preparation of RGD peptide-based polymers that has important implications in the synthesized biomaterials that support cell adhesion.

Improved Bone Cell Adhesion on Ultrafine Grained Titanium and Ti-6A1-4V

2012 ◽  
pp. 239-245
Author(s):  
Chang Yao ◽  
Elliott B. Slamovich ◽  
Javaid I. Qazi ◽  
Henry. J. Rack ◽  
Thomas J. Webster
Keyword(s):  
Cell Adhesion ◽  
Bone Cell ◽  
Ultrafine Grained

scholarly journals Multifunctional Properties of Quercitrin-Coated Porous Ti-6Al-4V Implants for Orthopaedic Applications Assessed In Vitro

2020 ◽  
Vol 9 (3) ◽  
pp. 855 ◽  
Author(s):  
Maria Antonia Llopis-Grimalt ◽  
Aina Arbós ◽  
Maria Gil-Mir ◽  
Aleksandra Mosur ◽  
Prathamesh Kulkarni ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Antibacterial Properties ◽  
Young Modulus ◽  
Orthopedic Implants ◽  
Antibacterial Activities ◽  
Bone Cell ◽  
Alp Activity ◽  
Porous Ti ◽  
Osteocalcin Production

(1) One strategy to improve the outcome of orthopedic implants is to use porous implants with the addition of a coating with an antibacterial biomolecule. In this study, we aimed to produce and test the biocompatibility, the osteopromotive (both under normal conditions and under a bacterial challenge with lipopolysaccharide (LPS)) and antibacterial activities of a porous Ti-6Al-4V implant coated with the flavonoid quercitrin in vitro. (2) Porous Ti-6Al-4V implants were produced by 3D printing and further functionalized with quercitrin by wet chemistry. Implants were characterized in terms of porosity and mechanical testing, and the coating with quercitrin by fluorescence staining. Implant biocompatibility and bioactivity was tested using MC3T3-E1 preosteoblasts by analyzing cytotoxicity, cell adhesion, osteocalcin production, and alkaline phosphatase (ALP) activity under control and under bacterial challenging conditions using lipopolysaccharide (LPS). Finally, the antibacterial properties of the implants were studied using Staphylococcus epidermidis by measuring bacterial viability and adhesion. (3) Porous implants showed pore size of about 500 µm and a porosity of 52%. The coating was homogeneous over all the 3D surface and did not alter the mechanical properties of the Young modulus. Quercitrin-coated implants showed higher biocompatibility, cell adhesion, and osteocalcin production compared with control implants. Moreover, higher ALP activity was observed for the quercitrin group under both normal and bacterial challenging conditions. Finally, S. epidermidis live/dead ratio and adhesion after 4 h of incubation was lower on quercitrin implants compared with the control. (4) Quercitrin-functionalized porous Ti-6Al-4V implants present a great potential as an orthopedic porous implant that decreases bacterial adhesion and viability while promoting bone cell growth and differentiation.

Selective bone cell adhesion on formulations containing carbon nanofibers

Biomaterials ◽  
2003 ◽  
Vol 24 (11) ◽  
pp. 1877-1887 ◽  
Author(s):  
Rachel L Price ◽  
Michael C Waid ◽  
Karen M Haberstroh ◽  
Thomas J Webster
Keyword(s):  
Cell Adhesion ◽  
Carbon Nanofibers ◽  
Bone Cell

Comparative cell adhesion properties of cysteine extended peptide architectures

RSC Advances ◽  
2016 ◽  
Vol 6 (4) ◽  
pp. 2695-2702 ◽  
Author(s):  
Saniye Soylemez ◽  
Bilal Demir ◽  
Gizem Oyman Eyrilmez ◽  
Seçkin Kesici ◽  
Aytül Saylam ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Attachment ◽  
Rgd Peptide ◽  
Adhesion Properties ◽  
Modified Surfaces

This study presents the comparative cell attachment investigation of TAT and well-known RGD peptide modified surfaces.

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