Expression analysis and study of KLK4 in benign and malignant breast tumours

2009 ◽  
Vol 101 (02) ◽  
pp. 381-387 ◽  
Author(s):  
Georgia Papachristopoulou ◽  
Margaritis Avgeris ◽  
Andreas Scorilas
Keyword(s):  
Expression Analysis ◽  
Housekeeping Gene ◽  
Mrna Levels ◽  
Breast Tumours ◽  
Potential Biomarker ◽  
Quantification Analysis ◽  
Malignant Breast ◽  
Well Differentiated ◽  
Tissue Specimens ◽  
Analysis And Study

SummaryThe steroid hormone-regulated gene KLK4 (kallikrein 4) is a new member of the human kallikrein-related peptidase gene family. Up to date, studies report that KLK4 is differentially expressed in many tumours. The purpose of this study was the expression analysis and study of KLK4 in benign and malignant breast tumours. Total RNA was isolated from 16 benign and 45 malignant breast tissue specimens. After testing RNA quality, cDNA was prepared by reverse transcription. Highly sensitive quantitative real-time PCR method for KLK4 mRNA quantification was developed using the SYBR Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative CT method 2-ΔΔCT. KLK4 expression was found to vary in both patients’ cohorts; however, a statistically significant elevation of the KLK4 mRNA levels was observed in malignant compared to benign tumour patients. Low KLK4 expression levels were found in well-differentiated tumours (p=0.011) as well as in stage I (p=0.024) patients. Moreover, a statistically significant (rs=-0.318, p=0.035) negative correlation between the KLK4 expression and progesterone receptor staining was observed. ROC and logistic regression analysis recommended that KLK4 gene expression may be used as a new potential biomarker in breast cancer.

Quantitative expression analysis and prognostic significance of the novel apoptosis-related gene BCL2L12 in colon cancer

2008 ◽  
Vol 389 (12) ◽  
Author(s):  
Christos K. Kontos ◽  
Iordanis N. Papadopoulos ◽  
Andreas Scorilas
Keyword(s):  
Colon Cancer ◽  
Early Stage ◽  
Critical Role ◽  
Prognostic Significance ◽  
Disease Free Survival ◽  
Housekeeping Gene ◽  
Colon Tissue ◽  
Quantitative Expression ◽  
Quantification Analysis ◽  
Well Differentiated

Abstract Apoptosis is a tightly regulated process that plays a critical role in many biological events. Members of the BCL2 (Bcl-2) family of apoptosis-related genes have been found to be differentially expressed in various malignancies and have been proposed as prognostic tumor biomarkers. We have recently discovered and cloned a new member of the BCL2 gene family, BCL2L12, expressed in colon tissues. Here we have investigated expression of the BCL2L12 gene in colon cancer tissues and assessed its prognostic value. Total RNA was isolated from 96 specimens of malignant colon tissue. After testing the RNA quality, cDNA was prepared by reverse transcription. A highly sensitive real-time PCR method for BCL2L12 mRNA quantification was developed using SYBR® Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative CT method (2-ΔΔCT). High BCL2L12 expression levels were found in smaller (≤5 cm, p=0.027) and well-differentiated tumors (p=0.034), as well as in early-stage tumors (p=0.039). Survival analysis demonstrated that patients with BCL2L12-positive colon tumors have significantly longer disease-free survival and overall survival (p=0.015 and p=0.027, respectively). Our results suggest that BCL2L12 gene expression may represent a potential new biomarker for colon cancer.

scholarly journals The usefulness of competitive PCR: airway gene expression of IL-5, IL-4, IL-4δ2, IL-2, and IFNγ in asthma

Thorax ◽  
2001 ◽  
Vol 56 (7) ◽  
pp. 541-548
Author(s):  
E M Glare ◽  
M Divjak ◽  
M J Bailey ◽  
E H Walters
Keyword(s):  
Gene Expression ◽  
Inhaled Corticosteroids ◽  
In Situ Hybridisation ◽  
Housekeeping Gene ◽  
Cross Sectional Study ◽  
Mrna Levels ◽  
Competitive Pcr ◽  
Cross Sectional ◽  
Steroid Use ◽  
Biopsy Specimens

BACKGROUNDAsthma has been described as an eosinophilic bronchitis driven by interleukin(IL)-4 and IL-5. The quantification of cytokine mRNA levels in airway samples has been confounded by housekeeping gene expression which differs between and within asthmatics and controls.METHODSThe usefulness of competitive reverse transcriptase-polymerase chain reaction (RT-PCR) that is independent of housekeeping gene expression for quantitating the mRNA for interferon (IFN)γ, IL-2, IL-5, IL-4 and its receptor antagonist encoding splicing variant IL-4δ2 was determined in a cross sectional study of 45 normal control subjects and 111 with asthma.RESULTSAtopic controls and atopic asthmatic subjects expressed more IL-5 than non-atopic controls (p<0.02) in bronchoalveolar lavage (BAL) cells, but not in biopsy specimens. IL-5 mRNA expression in BAL cells from asthmatic subjects using inhaled corticosteroids (ICS) was significantly lower than those not receiving ICS (p=0.04). IL-2 mRNA levels differed with steroid use in biopsy specimens but not in BAL cells. IFNγ, IL-4, and IL-4δ2 mRNA levels did not differ between any groups and were not affected by steroid use. IL-4 and IL-4δ2 mRNA levels were positively correlated (p<0.0001), suggesting coordinated transcription.CONCLUSIONSWhile the signal differentiation of competitive PCR in asthma may rival that of in situ hybridisation and immunohistochemistry, the method is expensive and wasteful of material.

scholarly journals CD163 as a Potential Biomarker of Monocyte Activation in Ischemic Stroke Patients

2021 ◽  
Vol 22 (13) ◽  
pp. 6712
Author(s):  
Rosaria Greco ◽  
Chiara Demartini ◽  
Anna Maria Zanaboni ◽  
Elena Tumelero ◽  
Alessandra Persico ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Stroke Severity ◽  
Mrna Levels ◽  
Monocyte Count ◽  
Stroke Patients ◽  
Inflammatory Status ◽  
Specific Distribution ◽  
Cd163 Expression ◽  
Potential Biomarker ◽  
Complex Correlation

In ischemic stroke patients, a higher monocyte count is associated with disease severity and worse prognosis. The complex correlation between subset phenotypes and functions underscores the importance of clarifying the role of monocyte subpopulations. We examined the subtype-specific distribution of the CD163+ and CD80+ circulating monocytes and evaluated their association with the inflammatory status in 26 ischemic stroke patients and 16 healthy controls. An increased percentage of CD163+/CD16+ and CD163+/CD14++ events occurred 24 and 48 h after a stroke compared to the controls. CD163+ expression was more pronounced in CD16+ non-classical and intermediate monocytes, as compared to CD14+ classical subtype, 24 h after stroke. Conversely, the percentage of CD80+/CD16+ events was unaffected in patients; meanwhile, the percentage of CD80+/CD14+ events significantly increased only 24 h after stroke. Interleukin (IL)-1beta, TNF-alpha, and IL-4 mRNA levels were higher, while IL-10 mRNA levels were reduced in total monocytes from patients versus controls, at either 24 h or 48 h after stroke. The percentage of CD163+/CD16+ events 24 h after stroke was positively associated with NIHSS score and mRS at admission, suggesting that stroke severity and disability are relevant triggers for CD163+ expression in circulating CD16+ monocytes.

scholarly journals Expression analisis of the ITSN2 and TKS5 mRNA isoforms in human malignant breast tumors

2018 ◽  
Vol 16 (1) ◽  
pp. 20-27
Author(s):  
S. V. Kropyvko ◽  
L. O. Tsyba ◽  
O. V. Novokhatska ◽  
L. A. Syvak ◽  
T. Ye. Tarasenko ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Expression Analysis ◽  
Rna Isolation ◽  
Breast Tumors ◽  
Quantitative Real Time Pcr ◽  
Mrna Isoforms ◽  
Extensive Experience ◽  
Great Progress ◽  
Malignant Breast ◽  
The Many

Aim. Despite the great progress in cancer treating, the breast cancer remains lethal in 15 % cases. Regardless of the many years of research and extensive experience in the treatment of this type of cancer, one of the main problems in diagnosis and therapy is its high clinical and genetic heterogeneity. Thereby the identification of markers for personalized treatment of patients is still an actual issue. Methods. Collection of clinical material, RNA isolation, and expression analysis of ITSN2 and TKS5 isoforms using quantitative real time PCR with fluorescence-labeled probes. Results. We have found that ITSN2-S expression is reliably reduced in HER2/neu-positive tumors with poor prognosis. There were no significant differences in the expression of ITSN2-L and TKS5-L in the analyzed samples. Conclusions. These studies have demonstrated the possible use of ITSN2 short isoform (ITSN2-S) as a prognostic marker for breast cancer. Keywords: breast cancer, ITSN2, TKS5, expression analysis.

scholarly journals Erythropoietin Receptor is a Risk Factor for Prognosis: A Potential Biomarker in Lung Adenocarcinoma

2022 ◽  
Author(s):  
Ya-Jing Zhang ◽  
Sen-Yu Wang ◽  
Song-Tao Han ◽  
Yao-Yao Huang ◽  
Yang-Chun Feng
Keyword(s):  
Lung Adenocarcinoma ◽  
Gene Mutations ◽  
Predictive Ability ◽  
Erythropoietin Receptor ◽  
Immunohistochemical Method ◽  
Normal Lung ◽  
Mrna Levels ◽  
Protein Levels ◽  
Potential Biomarker ◽  
Epor Expression

Abstract Background: Lung cancer has the highest mortality rate of all cancers, and LUAD's survival rate is particularly poor. Erythropoietin receptor (EPOR) is a member of the cytokine class I receptor family and can be detected in cancers such as lung adenocarcinoma (LUAD), however, the expression levels and prognostic value of EPOR in LUAD are still unclear.Methods: Multiple bioinformatics databases such as TIMER, Kaplan-Meier Plotter and TCGA databases, immunohistochemical method, and clinicopathological data of 92 LUADpatients between January 2008 and June 2016 were used to explore the EPOR expression, gene mutations affecting EPOR expression, EPOR-interacting or coexpressed genes, potential biological functions and the correlation of EPOR expression with prognosis, immune microenvironment and so on.All statistical analyses were performed in the R version 4.1.1.Results: In this study, the EPOR mRNA expression in LUAD tissues was possibly downregulated compared with that in normal lung tissues, but the EPOR protein expression in LUAD tissues was higher than that in paired normal lung tissues. Mutations in five genes, DDX60L, LGR6, POTEB3, RIF1 and SOX5, resulted in downregulation of EPOR expression, mutations in 10 genes includingC1orf168, DBX2 and EIF5B, resulted in upregulation of EPOR expression. Erichment analyses showed that EPOR is involved in neural tissue ligand-receptor interactions, MAPK and PI3K/Akt signaling pathways and cancer pathways. The KM Plotter and PrognoScan databases consistently concluded that EPOR was associated with prognosis in LUAD patients. Our clinicopathological data showed that high EPOR expression was associated with poorer OS (29.5 vs 46 months) and had a good predictive ability for 5-year survival probability. Conclusions: EPOR expression might be downregulated at the mRNA levels and significantly upregulated at the protein levels in LUAD, which showed that the mRNA and protein levels of EPOR are inconsistent.The high expression of EPOR was associated with poor prognosis and is expected to be a potential new prognostic marker for LUAD.

Evaluation of magnetic resonance imaging in premalignant and malignant breast tumours

1998 ◽  
Vol 7 ◽  
pp. S84
Author(s):  
H T Depypere ◽  
M Mortier ◽  
V Cocquyt ◽  
R Van den Broecke ◽  
C De Potter ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Breast Tumours ◽  
Malignant Breast

scholarly journals Prolactin Induced Protein (PIP) is a potential biomarker for early stage and malignant breast cancer

The Breast ◽  
2018 ◽  
Vol 39 ◽  
pp. 101-109 ◽  
Author(s):  
Anju Gangadharan ◽  
Themba Nyirenda ◽  
Kishan Patel ◽  
Nydia Jaimes-Delgadillo ◽  
Dominique Coletta ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Stage ◽  
Potential Biomarker ◽  
Malignant Breast

scholarly journals P947. Changes in surgical treatment of malignant breast tumours

The Breast ◽  
1997 ◽  
Vol 6 (5) ◽  
pp. 342
Author(s):  
I. Zöllei ◽  
Á. Balogh ◽  
J. Furák
Keyword(s):  
Surgical Treatment ◽  
Breast Tumours ◽  
Malignant Breast
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