scholarly journals PROLACTIN REDUCES HIPPOCAMPAL PARVALBUMIN AND GABAA RECEPTOR EXPRESSION IN FEMALE MICE

2021 ◽  
Author(s):  
Susana Mellado ◽  
Beatriz Moreno-Ruiz ◽  
Sara Exposito ◽  
Miriam Fernandez ◽  
Eduardo D. Martín
Keyword(s):  
Gabaa Receptor ◽  
Memory Task ◽  
Pituitary Hormones ◽  
Receptor Expression ◽  
Double Labelling ◽  
Female Mice ◽  
Inhibitory Circuits ◽  
Densitometry Analysis ◽  
Dependent Learning ◽  
Constituent Elements

Introduction: Parvalbumin (PV)-positive cells are strategic elements of neuronal networks capable of influencing memory and learning processes. However, it is not known whether pituitary hormones may be related to parvalbumin expression in the hippocampus — a part of the limbic system with important functions in learning and memory. Objective: Since previous studies indicate that prolactin (PRL) plays a significant role in hippocampal-dependent learning and synaptic plasticity, we hypothesized that a rise in PRL levels can modify PV expression in the hippocampus. Methods: We employed biochemical, immunohistochemistry and densitometry techniques —as well as a behavioural assay— in a hyperprolactinemia model using subcutaneous osmotic pumps in female mice. Results: PRL treatment via osmotic pump induced an increase in PRL receptor (PRLR) expression in most regions of hippocampus analysed by Western blotting and immunohistochemistry methods. Fluorescent densitometry analysis revealed that PV expression decreases in the same layers in the hippocampus following PRL treatment, while double labelling immunostaining indicated close localization of PV and PRLR in PV-positive interneurons. In addition, we found that PRL induced a reduction in β2/3 subunit of GABAA receptor (GABAAR) expression that was linearly correlated with the reduction in PV expression. This reduction in β2/3 subunit of GABAAR expression was maintained in trained animals in which PRL treatment improved the learning of a spatial memory task. Conclusions: These data show, for the first time, that an increase in PRL level is associated with changes in key constituent elements of inhibitory circuits in the hippocampus and may be of relevance for the alterations in cognitive function reported in hyperprolactinemia.

scholarly journals Developmental regulation of voltage-gated K+ channel and GABAA receptor expression in Bergmann glial cells

1994 ◽  
Vol 14 (5) ◽  
pp. 2503-2514 ◽  
Author(s):  
T Muller ◽  
JM Fritschy ◽  
J Grosche ◽  
GD Pratt ◽  
H Mohler ◽  
...  
Keyword(s):  
Gabaa Receptor ◽  
Glial Cells ◽  
Developmental Regulation ◽  
Receptor Expression ◽  
K Channel ◽  
Voltage Gated ◽  
Bergmann Glial Cells

scholarly journals Early-life adversity selectively impairs α2-GABAA receptor expression in the mouse nucleus accumbens and influences the behavioral effects of cocaine

2018 ◽  
Vol 141 ◽  
pp. 98-112 ◽  
Author(s):  
Scott J. Mitchell ◽  
Edward P. Maguire ◽  
Linda Cunningham ◽  
Benjamin G. Gunn ◽  
Matthias Linke ◽  
...  
Keyword(s):  
Nucleus Accumbens ◽  
Gabaa Receptor ◽  
Early Life ◽  
Receptor Expression ◽  
Behavioral Effects ◽  
Early Life Adversity

scholarly journals Stroke Increases G Protein-Coupled Estrogen Receptor Expression in the Brain of Male but Not Female Mice

Neurosignals ◽  
2013 ◽  
Vol 21 (3-4) ◽  
pp. 229-239 ◽  
Author(s):  
Brad R.S. Broughton ◽  
Vanessa H. Brait ◽  
Elizabeth Guida ◽  
Seyoung Lee ◽  
Thiruma V. Arumugam ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
G Protein ◽  
Receptor Expression ◽  
Estrogen Receptor Expression ◽  
Female Mice ◽  
G Protein Coupled ◽  
The Brain

Abstract 096: Progesterone Upregulates Endothelial Mineralocorticoid Receptor Expression Which Predisposes Female Mice to Obesity-Induced Endothelial Dysfunction

Hypertension ◽  
2018 ◽  
Vol 72 (Suppl_1) ◽  
Author(s):  
Jessica L Faulkner ◽  
Simone Kennard ◽  
Galina Antonova ◽  
Zsolt Bagi ◽  
Iris Jaffe ◽  
...  
Keyword(s):  
Endothelial Dysfunction ◽  
Mineralocorticoid Receptor ◽  
Receptor Expression ◽  
Female Mice

055 Novel sleep-dependent spatial memory and navigation task using Minecraft

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A23-A23
Author(s):  
Katharine Simon ◽  
Gregory Clemenson ◽  
Brandon Clayton ◽  
Jing Zhang ◽  
Elisabet Alzueta ◽  
...  
Keyword(s):  
Spatial Memory ◽  
Test Performance ◽  
Target Location ◽  
Spatial Navigation ◽  
Memory Task ◽  
Spatial Location ◽  
Delay Test ◽  
Location Accuracy ◽  
Median Split ◽  
Dependent Learning

Abstract Introduction Spatial navigation and memory are hippocampally-dependent and decrease with age, yet, ecologically-valid methods remain elusive. We developed an engaging and inherently flexible spatial navigation/memory task using the Minecraft platform to test sleep-dependent memory. We validated baseline performance and learning rates across two separate Minecraft environments. Methods Using a within-subjects design, twenty-two subjects experienced two conditions (wake/sleep) and two Minecraft environments, counterbalanced across subjects. At encoding, subjects learned the locations of 12 objects. Memory for object location accuracy and navigation route (distance between start and target; vector: angle of direction towards target location from start) was tested immediately and following 12-hours of wake or sleep. Post-hoc analyses were conducted using a median split on subjects’ Immediate test performance. Results There were no significant differences across conditions for the Immediate test performance (t(22) = .567, p = .576) across the two environments. Delayed test showed greater improvement in accuracy after sleep compared to wake (t(18) = 2.795, p=.012), no differences in distance or vector. Median split by Immediate test performance revealed that initially lower performance showed the greatest improvement after delay in the sleep group (t(18) = 2.818, p =.011), but not the wake group (t(18) = -1.051, p =.308). Additionally, these same subjects’ vector direction was initially worse at Immediate Test (t(18) = -2.9, p = .01), and improved with sleep, becoming equivalent to the better performers at Delay test (t(18) = -.336, p = .74). Conclusion We demonstrate a novel spatial navigation/memory tasks using Minecraft that shows sleep-dependent learning across two distinct environments. We showed enhancement of spatial location accuracy after a night of sleep compared to wake. We further demonstrate that with sleep, those with worse initial performance show the greatest memory and navigation improvement, consistent with other findings that sleep supports enhancement of weaker memories and extended to the spatial-domain. This novel platform can be used to evaluate spatial memory across the lifespan and within special clinical populations. Support (if any) NIH R01 AG061355

scholarly journals Neuronal Hyperexcitability and Reduction of GABAA-Receptor Expression in the Surround of Cerebral Photothrombosis

1996 ◽  
Vol 16 (5) ◽  
pp. 906-914 ◽  
Author(s):  
Klaus Schiene ◽  
Claus Bruehl ◽  
Karl Zilles ◽  
Meishu Qu ◽  
Georg Hagemann ◽  
...  
Keyword(s):  
Gabaa Receptor ◽  
Rose Bengal ◽  
Sensorimotor Cortex ◽  
Neuronal Excitability ◽  
Epileptic Seizures ◽  
Receptor Expression ◽  
Aminobutyric Acid ◽  
Brain Areas ◽  
Series Of Experiments ◽  
The Rose

Changes of neuronal excitability and γ-aminobutyric acid (GABAA)-receptor expression were studied in the surround of photothrombotic infarcts, which were produced in the sensorimotor cortex of the rat by using the rose bengal technique. In a first series of experiments, multiunit recordings were performed on anesthetized animals 2–3 mm lateral from the lesion. Mean discharge frequency was considerably higher in recordings from lesioned animals (>100 Hz in the first postlesional week) compared with control animals (mean, 15 Hz). These alterations were already present after 1 day but were most pronounced 3 to 7 days after lesion induction. Thereafter the hyperexcitability declined again, although it remained visible up to 4 months. In a second series of experiments, the GABAA-receptor expression was studied autoradiographically. This revealed a reduction of GABAA receptors in widespread brain areas ipsilateral to the lesion. The reduction was most pronounced in the first days after lesion induction and declined with longer intervals. It is concluded that cortical infarction due to photothrombosis leads to a long-lasting and widespread reduction of GABAA-receptor expression in the surround of the lesion, which is associated with an increased neuronal excitability. Such alterations may be responsible for epileptic seizures that can be observed in some patients after stroke and may contribute to neurologic deficits after stroke.

scholarly journals Impaired surface αβγ GABAA receptor expression in familial epilepsy due to a GABRG2 frameshift mutation

2013 ◽  
Vol 50 ◽  
pp. 135-141 ◽  
Author(s):  
Mengnan Tian ◽  
Davide Mei ◽  
Elena Freri ◽  
Ciria C. Hernandez ◽  
Tiziana Granata ◽  
...  
Keyword(s):  
Gabaa Receptor ◽  
Frameshift Mutation ◽  
Receptor Expression
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