Rinsing with statherin-derived peptide alters the proteome of the acquired enamel pellicle

2021 ◽  
Author(s):  
Even Akemi Taira ◽  
Carolina Ruis Ferrari ◽  
Gabriel Carvalho ◽  
Talita Mendes Oliveira Ventura ◽  
Tatiana Martini ◽  
...  
Keyword(s):  
Acquired Enamel Pellicle

scholarly journals Bioinspired caries preventive strategy via customizable pellicles of saliva-derived protein/peptide constructs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dina G. Moussa ◽  
Walter L. Siqueira
Keyword(s):  
Protective Role ◽  
Functional Domains ◽  
Preventive Strategy ◽  
Native Proteins ◽  
Bacterial Cell Death ◽  
Effective Care ◽  
Whole Saliva ◽  
Biofilm Dispersion ◽  
Financial Burdens ◽  
Acquired Enamel Pellicle

AbstractDental caries has been the most widespread chronic disease globally associated with significant health and financial burdens. Caries typically starts in the enamel, which is a unique tissue that cannot be healed or regrown; nonetheless, new preventive approaches have limitations and no effective care has developed yet. Since enamel is a non-renewable tissue, we believe that the intimate overlaying layer, the acquired enamel pellicle (AEP), plays a crucial lifetime protective role and could be employed to control bacterial adhesion and dental plaque succession. Based on our identified AEP whole proteome/peptidome, we investigated the bioinhibitory capacities of the native abundant proteins/peptides adsorbed in pellicle-mimicking conditions. Further, we designed novel hybrid constructs comprising antifouling and antimicrobial functional domains derived from statherin and histatin families, respectively, to attain synergistic preventive effects. Three novel constructs demonstrated significant multifaceted bio-inhibition compared to either the whole saliva and/or its native proteins/peptides via reducing biomass fouling and inducing biofilm dispersion beside triggering bacterial cell death. These data are valuable to bioengineer precision-guided enamel pellicles as an efficient and versatile prevention remedy. In conclusion, integrating complementary acting functional domains of salivary proteins/peptides is a novel translational approach to design multifunctional customizable enamel pellicles for caries prevention.

Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation

2022 ◽  
Vol 116 ◽  
pp. 103874
Author(s):  
Tamara Teodoro Araújo ◽  
Thamyris Souza Carvalho ◽  
Aline Dionizio ◽  
Ana Luiza Bogaz Debortolli ◽  
Talita Mendes Oliveira Ventura ◽  
...  
Keyword(s):  
Acquired Enamel Pellicle

Acquired Enamel Pellicle and Its Potential Role in Oral Diagnostics

2007 ◽  
Vol 1098 (1) ◽  
pp. 504-509 ◽  
Author(s):  
W. L SIQUEIRA ◽  
E. J HELMERHORST ◽  
W ZHANG ◽  
E SALIH ◽  
F. G OPPENHEIM
Keyword(s):  
Potential Role ◽  
Acquired Enamel Pellicle

Enzymes in the acquired enamel pellicle

2005 ◽  
Vol 113 (1) ◽  
pp. 2-13 ◽  
Author(s):  
Christian Hannig ◽  
Matthias Hannig ◽  
Thomas Attin
Keyword(s):  
Acquired Enamel Pellicle

Further Information on the Composition of the Acquired Enamel Pellicle

2015 ◽  
pp. 109-118
Author(s):  
Kristen Hannesson Eggen ◽  
Gunnar R�lla
Keyword(s):  
Acquired Enamel Pellicle

scholarly journals A New Sugarcane Cystatin Strongly Binds to Dental Enamel and Reduces Erosion

2017 ◽  
Vol 96 (9) ◽  
pp. 1051-1057 ◽  
Author(s):  
A.C. Santiago ◽  
Z.N. Khan ◽  
M.C. Miguel ◽  
C.C. Gironda ◽  
A. Soares-Costa ◽  
...  
Keyword(s):  
Potent Inhibitor ◽  
Surface Hardness ◽  
Dental Enamel ◽  
Reversible Inhibitors ◽  
Force Microscopy ◽  
Atomic Force ◽  
Enamel Erosion ◽  
Acquired Enamel Pellicle

Cystatin B was recently identified as an acid-resistant protein in acquired enamel pellicle; it could therefore be included in oral products to protect against caries and erosion. However, human recombinant cystatin is very expensive, and alternatives to its use are necessary. Phytocystatins are reversible inhibitors of cysteine peptidases that are found naturally in plants. In plants, they have several biological and physiological functions, such as the regulation of endogenous processes, defense against pathogens, and response to abiotic stress. Previous studies performed by our research group have reported high inhibitory activity and potential agricultural and medical applications of several sugarcane cystatins, including CaneCPI-1, CaneCPI-2, CaneCPI-3, and CaneCPI-4. In the present study, we report the characterization of a novel sugarcane cystatin, named CaneCPI-5. This cystatin was efficiently expressed in Escherichia coli, and inhibitory assays demonstrated that it was a potent inhibitor of human cathepsins B, K, and L ( Ki = 6.87, 0.49, and 0.34 nM, respectively). The ability of CaneCPI-5 to bind to dental enamel was evaluated using atomic force microscopy. Its capacity to protect against initial enamel erosion was also tested in vitro via changes in surface hardness. CaneCPI-5 showed a very large force of interaction with enamel (e.g., compared with mucin and casein) and significantly reduced initial enamel erosion. These results suggest that the inclusion of CaneCPIs in dental products might confer protection against enamel erosion.

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