LncRNA PMS2L2 downregulates miR-24 through methylation to suppress cell apoptosis in ulcerative colitis

2020 ◽  
Author(s):  
Ting Yu ◽  
Fanyu Meng ◽  
Minning Xie ◽  
Huajiang Liu ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Cell Apoptosis ◽  
Healthy Controls ◽  
Western Blots ◽  
Age And Gender ◽  
Specific Pcr ◽  
Rna Immunoprecipitation ◽  
Healthy Control ◽  
And Gender

Background: Ulcerative colitis (UC) is an inflammatory bowel disease characterized by chronic inflammation of the colon. It has been reported that PMS2L2 plays protective roles in inflammatory injury. This study aimed to investigate the role of lncRNA PMS2L2 in UC. Methods: 62 patients with UC as well as 62 age- and gender- matched healthy controls were enrolled. Expressions of PMS2L2 and miR-24 in plasma from UC patients and healthy controls were determined by RT-qPCR. The interaction between PMS2L2 and miR-24 was predicted by bioinformatics and confirmed by RNA immunoprecipitation (RIP) and RNA pull-down. The role of PMS2L2 in the regulation of miR-24 gene methylation was analyzed by methylation-specific PCR (MSP). The effects of PMS2L2 and miR-24 on the expressions of apoptosis-related proteins were detected by western blots. Results: PMS2L2 was downregulated in the plasma of UC patients compared to that in age- and gender- matched healthy control. In HCnEpCs, PMS2L2 overexpression inhibited miR-24 expression via promoting the methylation of miR-24 gene. In contrast, miR-24 overexpression failed to affect PMS2L2. In the detection of cell apoptosis, PMS2L2 overexpression could promote the expression of Bcl-2 and inhibit Bax, cleaved-caspase-3 and cleaved-caspase-9 expressions stimulated by LPS. Flow cytometer revealed that PMS2L2 elevation suppressed the apoptosis of HCnEpCs induced by LPS, but miR-24 aggravated the apoptosis. PMS2L2 overexpression rescued the detrimental effect of miR-24 on cell apoptosis. Conclusion: PMS2L2 may downregulate miR-24 via methylation to suppress cell apoptosis in UC.

scholarly journals CircRNA MFACR is Upregulated in Myocardial Infarction and Downregulates miR-125b to Promote Cardiomyocyte Apoptosis-induced by Hypoxia

2020 ◽  
Author(s):  
Shujuan Wang ◽  
Long Li ◽  
Weijie Deng ◽  
Minhua Jiang
Keyword(s):  
Myocardial Infarction ◽  
Cell Apoptosis ◽  
Cardiomyocyte Apoptosis ◽  
Healthy Controls ◽  
Plasma Samples ◽  
Methylation Specific Pcr ◽  
Specific Pcr ◽  
Apoptosis Assay ◽  
Hypoxia Treatment

Abstract Background: CircRNA MFACR promotes cardiomyocyte death, which contributes to myocardial infarction (MI). We analyzed the role of MFACR in MI.Methods: RT-qPCR was applied to determine MFACR and miR-125b expression in plasma samples from both MI patients (n=61) and healthy controls (n=61). MFACR and miR-125b were overexpressed in AC16 cells (cardiomyocytes) to study the interactions between them. Methylation of miR-125b gene in cells with MFACR overexpression was analyzed by methylation-specific PCR. Cell apoptosis after transfections was analyzed by cell apoptosis assay.Results: MFACR was overexpressed in MI and inversely correlated with miR-125b. In AC16 cells, hypoxia treatment increased MFACR expression and decreased miR-125b expression. In AC16 cells, MFACR overexpression decreased miR-125b expression and increased the methylation of miR-125b gene. Under hypoxia, MFACR overexpression increased AC16 cell apoptosis, and miR-125b overexpression decreased cell apoptosis. In addition, miR-125b overexpression reversed the effects of MFACR overexpression on cell apoptosis. Conclusion: MFACR may increase the methylation of miR-125b gene to downregulate its expression, thereby promoting the apoptosis of cardiomyocytes in MI.

scholarly journals The IL-17A rs2275913 polymorphism is associated with colorectal cancer risk

2020 ◽  
Vol 48 (12) ◽  
pp. 030006052097911
Author(s):  
Shulong Zhang ◽  
Xiaoting Wang
Keyword(s):  
Colorectal Cancer ◽  
Logistic Regression ◽  
Fragment Length Polymorphism ◽  
Pooled Analysis ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Age And Gender ◽  
Polymerase Chain ◽  
And Gender

Objective The association of the IL-17A rs2275913 polymorphism with the risk of colorectal cancer (CRC) has been previously reported. However, the results are inconsistent. In this study, we comprehensively assessed the effect of the rs2275913 polymorphism on CRC risk. Methods The rs2275913 polymorphism of 208 CRC patients and 312 age- and gender-matched healthy controls was genotyped by the polymerase chain reaction-restriction fragment length polymorphism method, and then analyzed by logistic regression. In addition, a pooled analysis based on five single-center studies was performed using Stata 12.0 software. Results Logistic regression analysis indicated that the IL-17A rs2275913 polymorphism was associated with CRC risk (GA vs. GG: OR = 1.53, 95% CI = 1.02–2.28; AA vs. GG: OR = 1.89, 95% CI = 1.11–3.20; GA+AA vs. GG: OR = 1.62, 95% CI = 1.11–2.37; A vs. G: OR = 1.38, 95% CI = 1.07–1.77). Further pooled analysis also indicated a statistically significant association between the rs2275913 polymorphism and CRC risk in Asians and Northern Africans. Conclusion This study suggested that the IL-17A rs2275913 polymorphism may act as a biomarker for predicting CRC risk. However, further functional research should be performed to clarify the role of the rs2275913 polymorphism in the etiology of CRC.

scholarly journals Association Analysis of COQ2 Variant in Dementia and Essential Tremor

2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Yin Xia Chao ◽  
Ebonne Yu Lin Ng ◽  
Huihua Li ◽  
Kandiah Nagaendran ◽  
Yuen Yih ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Confidence Interval ◽  
Multiple System Atrophy ◽  
Essential Tremor ◽  
Association Analysis ◽  
Odds Ratio ◽  
Healthy Controls ◽  
Age And Gender ◽  
And Gender

Objective. COQ2 mutations have been reported in Japanese multiple system atrophy (MSA) patients. We examined the role of COQ2 in patients with dementia and essential tremor (ET), two common neurodegenerative conditions.Materials & Methods. A total of 2064 subjects, including 560 patients with dementia, 466 patients with ET, and 1038 healthy controls, were included. Genotyping for the COQ2 V393A (T>C) was carried out. Odds ratio (OR) adjusted by age and gender, together with 95% confidence interval (CI), was reported by means of logistic regression.Results. The frequency of the polymorphic variant V393A heterozygous (T/C) was 2.7% in dementia, 1.1% in ET, and 2.5% in controls (OR = 0.70, 95% confidence interval is 0.29–1.72 for dementia, and OR = 0.47, 95% confidence interval is 0.17–1.31,p=0.1217for ET). There was no significant association between V393A variant with dementia and ET.Conclusion. There was no significant association between V393A variant with dementia and ET. COQ2 gene is unlikely to play a significant role in patients with dementia or ET in our population.

scholarly journals Swallowing in Parkinson Patients versus Healthy Controls: Reliability of Measurements in Videofluoroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Laura W. J. Baijens ◽  
Renée Speyer ◽  
Valéria Lima Passos ◽  
Walmari Pilz ◽  
Nel Roodenburg ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Interrater Reliability ◽  
Matched Control ◽  
Healthy Controls ◽  
Swallowing Function ◽  
Age And Gender ◽  
Control Subjects ◽  
Healthy Control ◽  
And Gender

Objective. To determine and describe the pathophysiological aspects of oropharyngeal swallowing in patients with Parkinson's disease more accurately, a pilot study of qualitative as well as quantitative parameters of swallowing was performed using videofluoroscopy (VFS).Methods. Ten patients with a diagnosis of idiopathic Parkinson's disease having dysphagic complaints and ten healthy age- and gender-matched control subjects underwent a standardized videofluoroscopic swallowing protocol. Information on the swallowing function was derived from temporal, spatial, and descriptive visuoperceptual parameters. Intra- and interrater reliability was calculated.Results. No significant differences were found between Parkinson patients and healthy control subjects for the majority of the reliable variables.Conclusions. It was concluded that swallowing function seemed to be preserved in the early stages of Parkinson's disease. Furthermore, the reliability of many quantitative as well as qualitative swallowing parameters proved insufficient, raising questions about the interpretation of study outcomes in videofluoroscopy.

scholarly journals CircRNA MFACR is Upregulated in Myocardial Infarction and Downregulates miR-125b to Promote Cardiomyocyte Apoptosis-induced by Hypoxia

2020 ◽  
Author(s):  
Shujuan Wang ◽  
Long Li ◽  
Weijie Deng ◽  
Minhua Jiang
Keyword(s):  
Myocardial Infarction ◽  
Cell Apoptosis ◽  
Cardiomyocyte Apoptosis ◽  
Healthy Controls ◽  
Plasma Samples ◽  
Methylation Specific Pcr ◽  
Specific Pcr ◽  
Apoptosis Assay ◽  
Hypoxia Treatment

Abstract Background：CircRNA MFACR promotes cardiomyocyte death, which contributes to myocardial infarction (MI). This study was carried out to explore the involvement of MFACR in MI.RT-qPCRs were performed to analyze the expression of MFACR and miR-125b in plasma samples from both MI patients (n=61) and healthy controls (n=61). MFACR and miR-125b were overexpressed in AC16 cells (cardiomyocytes) to study the interactions between them. The role of MFACR in regulating the methylation of miR-125b gene was analyzed by methylation-specific PCR. Cell apoptosis after transfections was analyzed by cell apoptosis assay.Result ：MFACR was overexpressed in MI and inversely correlated with miR-125b. In AC16 cells, hypoxia treatment increased MFACR expression and decreased miR-125b expression. In AC16 cells, MFACR overexpression decreased miR-125b expression and increased the methylation of miR-125b gene. Under hypoxia, MFACR overexpression increased AC16 cell apoptosis, and miR-125b overexpression decreased cell apoptosis. In addition, miR-125b overexpression reversed the effects of MFACR overexpression on cell apoptosis.Conclusions： MFACR may downregulate miR-125b through methylation to promote the apoptosis of cardiomyocytes in MI.

Empirical Analysis of Clinical Evaluations on Children Involved in the Child Protection System: The Role of Age and Gender

2000 ◽  
Author(s):  
Erika Felix ◽  
Anjali T. Naik-Polan ◽  
Christine Sloss ◽  
Lashaunda Poindexter ◽  
Karen S. Budd
Keyword(s):  
Empirical Analysis ◽  
Child Protection ◽  
Protection System ◽  
Age And Gender ◽  
Clinical Evaluations ◽  
And Gender

Children’s Lives and Deaths in Merovingian Gaul

2020 ◽  
pp. 185-213
Author(s):  
Émilie Perez
Keyword(s):  
Social Identity ◽  
Age Groups ◽  
New Method ◽  
Recent Analysis ◽  
Age And Gender ◽  
The Past ◽  
And Gender ◽  
Grave Goods

The role of children in Merovingian society has long been downplayed, and the study of their graves and bones has long been neglected. However, during the past fifteen years, archaeologists have shown growing interest in the place of children in Merovingian society. Nonetheless, this research has not been without challenges linked to the nature of the biological and material remains. Recent analysis of 315 children’s graves from four Merovingian cemeteries in northern Gaul (sixth to seventh centuries) allows us to understand the modalities of burial ritual for children. A new method for classifying children into social age groups shows that the type, quality, quantity, and diversity of grave goods were directly correlated with the age of the deceased. They increased from the age of eight and particularly around the time of puberty. This study discusses the role of age and gender in the construction and expression of social identity during childhood in the Merovingian period.

scholarly journals Physical Activity and Quality of Life in Hemodialysis Patients and Healthy Controls: A Cross-Sectional Study

2021 ◽  
Vol 18 (4) ◽  
pp. 1978
Author(s):  
Tjaša Filipčič ◽  
Špela Bogataj ◽  
Jernej Pajek ◽  
Maja Pajek
Keyword(s):  
Quality Of Life ◽  
Physical Activity ◽  
Everyday Life ◽  
Habitual Physical Activity ◽  
Healthy Controls ◽  
Age And Gender ◽  
Comorbidity Score ◽  
Sf 36 ◽  
And Gender

Hemodialysis (HD) patients have lower functional abilities compared to healthy people, and this is associated with lower physical activity in everyday life. This may affect their quality of life, but research on this topic is limited. Therefore, the present study aimed to determine the relationship between habitual physical activity and quality of life in HD patients and healthy controls. Ninety-three HD patients and 140 controls participated in the study. Quality of life was assessed using a 36-item medical outcomes study short-form health survey (SF-36). Human Activity Profile (HAP) was used to assess habitual physical activity. The adjusted activity score (AAS) from HAP, age, gender, fat tissue index (FTI), lean tissue index (LTI), and Davies comorbidity score were analyzed as possible predictors of the Physical Component Summary (PCS) of the SF-36. Three sequential linear models were used to model PCS. In Model 1, PCS was regressed by gender and age; in Model 2 the LTI, FTI, and Davies comorbidity scores were added. Model 3 also included AAS. After controlling for age and gender (ModelHD 1: p = 0.056), LTI, FTI, and Davies comorbidity score effects (ModelHD 2: p = 0.181), the AAS accounted for 32% of the variation in PCS of HD patients (ModelHD 3: p < 0.001). Consequently, the PCS of HD patients would increase by 0.431 points if the AAS increased by one point. However, in healthy controls, AAS had a lower impact than in the HD sample (B = 0.359 vs. 0.431), while the corresponding effects of age and gender (ModelH 1: p < 0.001), LTI, FTI, and Davies comorbidity score (ModelH 2: p < 0.001) were adjusted for. The proportion of variation in PCS attributed to AAS was 14.9% (ModelH 3: p < 0.001). The current study results showed that physical activity in everyday life as measured by the HAP questionnaire is associated to a higher degree with the quality of life of HD patients than in healthy subjects. Routine physical activity programs are therefore highly justified, and the nephrology community should play a leading role in this effort.

scholarly journals Silencing of MEG3 attenuated the role of lipopolysaccharides by modulating the miR-93-5p/PTEN pathway in Leydig cells

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Xu Zhou ◽  
Jingliang He ◽  
Jinbo Chen ◽  
Yu Cui ◽  
Zhenyu Ou ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Apoptosis ◽  
Leydig Cells ◽  
Treatment Strategies ◽  
Pten Expression ◽  
Luciferase Reporter ◽  
Western Blots ◽  
New Treatment ◽  
Lps Treatment

Abstract Background Leydig cells reflect the activation of inflammation, decrease of androgen production, inhibition of cell growth and promotion of cell apoptosis under orchitis. Maternally expressed gene 3 (MEG3) exerts a crucial role in various human diseases, but under orchitis, the role and underlying molecular mechanism of MEG3 in Leydig cells remain unclear. Methods Lipofectamine 2000 was used for the cell transfections. qPCR and western blots assay were applied to assess the gene expression. ELISA assay was used to measure the TNFα, IL6 and testosterone secretion. CCK8 and EdU assay was employ to test the cell viability and proliferation respectively. Luciferase reporter and RIP assay were introduced to detect the binding of miR-93-5p with MEG3 and PTEN. Results Lipopolysaccharides (LPS) induced TNFα and IL6 secretion, lowered testosterone production, inhibited cell viability and proliferation, and induced cell apoptosis in Leydig cells. MEG3 was upregulated in Leydig cells treated with LPS and that knockdown of MEG3 inhibited the role of LPS in Leydig cells. MEG3 absorbed miR-93-5p and that suppression of miR-93-5p restored the role of silenced MEG3 in Leydig cells under LPS treatment. miR-93-5p inhibited PTEN expression and that over-expressed PTEN alleviated the effect of miR-93-5p in Leydig cells treated with LPS. LPS activated the MEG3/miR-93-5p/PTEN signalling pathway in Leydig cells. Conclusions This study revealed that MEG3 serves as a molecular sponge to absorb miR-93-5p, thus leading to elevation of PTEN expression in Leydig cells under LPS treatment, offering a theoretical basis on which to establish potential new treatment strategies for orchitis.

Do They Really Mean It? Children’s Inference of Speaker Intentions and the Role of Age and Gender

2019 ◽  
Vol 63 (4) ◽  
pp. 689-712
Author(s):  
K. Rothermich ◽  
O. Caivano ◽  
L.J. Knoll ◽  
V. Talwar
Keyword(s):  
Gender Differences ◽  
Young Adults ◽  
Video Clip ◽  
Age And Gender ◽  
Video Clips ◽  
Age And Gender Differences ◽  
Speaker Intentions ◽  
And Gender

Interpreting other people’s intentions during communication represents a remarkable challenge for children. Although many studies have examined children’s understanding of, for example, sarcasm, less is known about their interpretation. Using realistic audiovisual scenes, we invited 124 children between 8 and 12 years old to watch video clips of young adults using different speaker intentions. After watching each video clip, children answered questions about the characters and their beliefs, and the perceived friendliness of the speaker. Children’s responses reveal age and gender differences in the ability to interpret speaker belief and social intentions, especially for scenarios conveying teasing and prosocial lies. We found that the ability to infer speaker belief of prosocial lies and to interpret social intentions increases with age. Our results suggest that children at the age of 8 years already show adult-like abilities to understand literal statements, whereas the ability to infer specific social intentions, such as teasing and prosocial lies, is still developing between the age of 8 and 12 years. Moreover, girls performed better in classifying prosocial lies and sarcasm as insincere than boys. The outcomes expand our understanding of how children observe speaker intentions and suggest further research into the development of teasing and prosocial lie interpretation.

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