scholarly journals Ethical Issues and Management of Fetal Hemolytic Anemia Caused by Anti-Rh17 in a Multipara with Rare –D– Phenotype

2021 ◽  
pp. 1-5
Author(s):  
Patrick P. Torreiter ◽  
Susanne Macher ◽  
Eva-Maria Matzhold ◽  
Bernhard Resch ◽  
Philipp Klaritsch ◽  
...  
Keyword(s):  
Blood Group ◽  
Hemolytic Anemia ◽  
Ethical Issues ◽  
Molecular Genetic ◽  
Peak Systolic Velocity ◽  
Intravenous Immunoglobulins ◽  
Molecular Genetic Analysis ◽  
Fetal Anemia ◽  
Blood Group Typing ◽  
Antibody Titers

<b><i>Background:</i></b> The development of allo-anti-Rh17 (anti-Hr0) in a –D– phenotype whose red blood cells (RBCs) lack CcEe antigens is most likely triggered by transfusion, transplantation, or pregnancy. Gene conversion is the predominating factor in generating RHD-CE-D and RHCE-D-CE hybrids like –D–. <b><i>Methods:</i></b> We report here immunohematological and obstetrical data from 2 of the 5 pregnancies of a 24-year-old woman presenting with the –D– phenotype with anti-Rh17. Blood group typing, antibody screening, antibody differentiation, direct antiglobulin test (DAT), and antibody titers were performed by routine gel technology and tube testing. Additionally, molecular genetic analysis was performed. Fetal surveillance was done by sonographic evaluation of the fetal middle cerebral artery peak systolic velocity (MCA-PSV). <b><i>Results:</i></b> Blood group typing showed O, C-c-D+E-e- and the DAT was negative. DNA sequencing revealed homozygosity for an <i>RHCE-D(3–9)-CE</i> null allele. Anti-Rh17 titers in the fourth pregnancy remained between 1:8 and 1:128, and no signs for a fetal anemia were observed. However, in the fifth pregnancy, the antibody titers increased up to 1:4,096. Signs of moderate fetal anemia were detected and cesarean section was performed at 34 + 6 weeks of gestation. The newborn presented with hemolytic anemia (cord blood hemoglobin [Hb] = 8.5 mg/dL). She received 2 compatible (small) packed RBC concentrates, phototherapy, and intravenous immunoglobulins. <b><i>Conclusion:</i></b> Our case shows that the risk for hemolytic complications increases with the number of pregnancies of sensitized women. Only people who also lack CcEe antigens are compatible as donors. The role of such rare donors as lifesavers, their freedom, and voluntariness conflict with the urgent need for compatible blood.

scholarly journals Molecular Genetic Analysis of the Human Lewis Histo-blood Group System

1996 ◽  
Vol 271 (16) ◽  
pp. 9830-9837 ◽  
Author(s):  
Takashi Kudo ◽  
Hiroko Iwasaki ◽  
Shoko Nishihara ◽  
Naoko Shinya ◽  
Takao Ando ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Group System

scholarly journals Molecular genetic analysis of the Kidd blood group system in a Taiwanese family with Jknull phenotype

2017 ◽  
Vol 1 (4) ◽  
pp. 29-35
Author(s):  
Fang-Yeh Chu ◽  
◽  
Lung-Chih Yu ◽  
Chih-Chun Chang ◽  
Marie Lin
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Group System

scholarly journals FEATURES OF THE IMMUNOGENETIC STRUCTURE OF PIGS OF DOMESTIC BREEDS, SUITABLE FOR XENOTRANSPLANTATION

2020 ◽  
Vol 59 ◽  
pp. 105-114
Author(s):  
O. I. Metlytska ◽  
T. N. Ryk ◽  
V. I. Rossokha ◽  
A. A. Saenko
Keyword(s):  
Blood Group ◽  
Molecular Genetic ◽  
Statistical Processing ◽  
Molecular Genetic Analysis ◽  
Blood Groups ◽  
Marker Allele ◽  
Allele Distribution ◽  
Specialized Line ◽  
Selection Of ◽  
Immunogenetic Analysis

The aim of the work was to determine the immunogenetic characteristics of pigs of two Ukrainian breeds by their adaptability, resistance, reproduction, genetic homogeneity, the presence of alleles that determine the potential suitability for use in xenotransplantation and other biomedical purposes. At present, there is no breed or specialized line of pigs in Ukraine to address the urgent problems of humane medicine. However, there is a favorable situation for the creation of such a breed or the withdrawal of a specialized line because of the demand of pharmacological concerns for model biological objects to study the mechanisms of action of modern medical preparations, to develop methods of conducting bloodless surgical operations, modeling of the clinic and epidemiology of infectious diseases of different diseases. immune response, etc. In this context, there was a compelling reason to preserve the indigenous breeds of pigs of Ukraine, especially Ukrainian meat and Мyrgorod. Standard methods of immunogenetic analysis of pigs were used. The erythrocyte antigens of 9 blood group systems were determined using specific immune sera and the involvement of a bank of immunodiagnostics meeting international requirements. Blood groups were determined by the reaction of agglutination, an indirect Coombs test, and a hemolytic test. Statistical processing of the research results was performed using mathematical statistics using the GenAlex-6.0 computer program. As a result of the immunogenetic analysis of Ukrainian beef and myrrh-pig breeds, it was determined that each of them is characterized by a specific immunogenic profile, which is related to both breed characteristics and differences, and methods of their breeding. The difference between the distribution of the overwhelming number of alleles of blood groups was statistically significant. Pigs of Ukrainian meat and Мyrgorod breeds had the largest differences in allele distribution by B, E, F, K, L blood group systems with the presence of the Ladhjk marker allele in the latter (p < 0.05). The specificity of immunogenic profiles was evaluated, depending on the history of creation and the direction of the animal's performance. Species of Myrhorod and Ukrainian meat breeds with the presence of genotypes A - / - and Ebdgkmp / bdgkmp were found to determine the suitability of animals for xenotransplantation. Of the 80 animals tested for immunogenetic markers, only 24 individuals met the established selection criteria. The range of determined indicators of actual homozygosity for selected genotype animals ranged from 55.56% to the maximum value – 88.89%, mainly in sows from the Smorodinа, Rusalkа, Sorokа and Soyka families. According to molecular genetic analysis, you have been informed that the established criteria of the biomedical model meet only 13 individuals of Ukrainian meat breed. Among animals with the highest index of actual homozygosity, representatives of Cererа and Cіlina were identified, with Celina 4092 being homozygous for all blood group systems studied. The selection of pigs for blood alleles, desirable for xenotransplantation, with their transfer to the homozygous state: A -/- and Ebdgkmp / bdgkmp will impair the adaptive, first of all, reproductive qualities of the animals and create significant problems for the cultivation of such individuals. The possibility of the occurrence of reproductive and resistance disorders of pigs in the selection of blood groups genotypes modeled for xenotransplantation and the search for ways to overcome them are being considered.

Molecular genetic analysis of weak B blood group allele in Jinan population reveals distribution of ABw phenotype

2021 ◽  
Vol 5 (1) ◽  
pp. 53-56
Author(s):  
Wu Wen ◽  
Li Xiaojian ◽  
Guo Xingying ◽  
Wu Peng ◽  
Huang Xiangyan
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Group Allele ◽  
B Blood Group

Molecular genetic analysis of ABO blood group variations reveals 29 novel ABO subgroup alleles

Transfusion ◽  
2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Xiaohong Cai ◽  
Sha Jin ◽  
Xi Liu ◽  
Liangfeng Fan ◽  
Qiong Lu ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Abo Blood Group

scholarly journals Molecular genetic analysis of variant phenotypes of the ABO blood group system

Blood ◽  
1996 ◽  
Vol 88 (7) ◽  
pp. 2732-2737 ◽  
Author(s):  
K Ogasawara ◽  
R Yabe ◽  
M Uchikawa ◽  
N Saitou ◽  
M Bannai ◽  
...  
Keyword(s):  
Blood Group ◽  
Molecular Genetic ◽  
Nonsynonymous Substitution ◽  
Single Strand Conformation Polymorphism ◽  
Molecular Genetic Analysis ◽  
Nucleotide Sequences ◽  
Activity Level ◽  
Blood Group System ◽  
Nucleotide Substitutions ◽  
Group System

ABO is clinically the most important blood group system in transfusion medicine and includes many variant phenotypes. To understand the molecular genetic basis of this polymorphic system, we have analyzed genomic DNAs obtained from Japanese individuals possessing variant ABO phenotypes including A2, Ax, Ael, cis-AB, Bx, and Bel. By polymerase chain reaction-single-strand conformation polymorphism (SSCP) and nucleotide sequence analyses, we identified 11 different alleles. These alleles had nucleotide sequences different from those of the previously described 13 different alleles responsible for the common ABO phenotypes. Analysis of the nucleotide sequences of the alleles responsible for those variant phenotypes showed that the amino acid residues at position 266 and 268 may be crucial for transferase specificity, whereas those at positions 214, 216, 223, 291, and 352 may be critical for the activity level. Nine of the 11 alleles, responsible for the A2, Ax, Ael, cis-AB, Bx, and Bel phenotypes, were presumed to be generated from common ABO alleles by single nucleotide mutations such as nonsynonymous substitution, deletion, or insertion. Two other alleles, responsible for the A2 and Ael phenotypes, may have originated by recombination, gene conversionlike events or accumulation of nucleotide substitutions. Our data indicate that different alleles could cause the same ABO variant phenotypes, and that these alleles do not necessarily belong to a single evolutionary lineage.

scholarly journals Molecular genetic analysis of a hybrid gene encoding Sta glycophorin of the human erythrocyte membrane

Blood ◽  
1989 ◽  
Vol 74 (2) ◽  
pp. 836-843
Author(s):  
CH Huang ◽  
ML Guizzo ◽  
M Kikuchi ◽  
OO Blumenfeld
Keyword(s):  
Blood Group ◽  
Erythrocyte Membrane ◽  
Molecular Genetic ◽  
Dna Typing ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Sequence Specificity ◽  
Antigen Specificity ◽  
Hybrid Gene ◽  
Junction Site

Sta is an antigen of the human MNSs blood group system carried by a variant glycophorin residing in the erythrocyte membrane. We examined the structure, organization, and inheritance of Sta gene identified in genomic DNA from an Oriental family. Southern blotting detected a useful genetic marker tightly linked to the Sta gene. Differential hybridization and secondary restriction analyses showed that Sta gene is a fusion hybrid of delta and alpha glycophorin genes. Genomic mapping by extensive use of synthetic oligonucleotides, with overlapping sequence specificity, allowed us to define the delta-alpha junction site and disclose the organization of the variant gene. The junction point of Sta hybrid gene is encompassed by an unexpressed exonlike sequence of the delta gene at the 5′ site, and an expressed sequence of the alpha gene spanning codons 59 through 71, at the 3′ site. Dosage quantification demonstrated the occurrence of Sta gene as a single copy in the genome. Blood group inheritance, evaluated by DNA typing, established the tight linkage of Sta to the alpha M and delta S genes. The data support a single unequal crossing-over event between misaligned delta and alpha genes on the homologous chromosomes as the mechanism for the origin of Sta gene. The Sta gene is similar in overall structure to another delta-alpha hybrid gene, Dantu, but differs from it in junction structure, copy number, gene linkage, and antigen specificity.

Molecular Genetic Analysis of the ABO Blood Group System: 4. Another Type of O Allele

Vox Sanguinis ◽  
1993 ◽  
Vol 64 (3) ◽  
pp. 175-178 ◽  
Author(s):  
Fumi-ichiro Yamamoto ◽  
Patricia D. McNeill ◽  
Miyako Yamamoto ◽  
Sen-itiroh Hakomori ◽  
Imelda M. Bromilow ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Abo Blood Group ◽  
Blood Group System ◽  
Group System

scholarly journals Molecular Genetic Analysis of the ABO Blood Group System: 1. Weak Subgroups: A^3 and B^3 Alleles

Vox Sanguinis ◽  
1993 ◽  
Vol 64 (2) ◽  
pp. 116-119
Author(s):  
Fumi-ichiro Yamamoto ◽  
Patricia D. McNeill ◽  
Miyako Yamamoto ◽  
Sen-itiroh Hakomori ◽  
Teresa Harris ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Abo Blood Group ◽  
Blood Group System ◽  
Group System ◽  
System 1

scholarly journals Molecular genetic analysis of the human Lewis histo-blood group system.

1994 ◽  
Vol 269 (46) ◽  
pp. 29271-29278
Author(s):  
S Nishihara ◽  
H Narimatsu ◽  
H Iwasaki ◽  
S Yazawa ◽  
S Akamatsu ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Group System
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