Dose-Response Effect of Fluoride Dentifrices on De-/Remineralization of Root Dentine in situ

2020 ◽  
Vol 54 (5-6) ◽  
pp. 502-508
Author(s):  
José Leal ◽  
Robson Ferreira ◽  
Cinthia Tabchoury ◽  
Peter Lingström ◽  
Glauber Vale
Keyword(s):  
Dose Response ◽  
Sucrose Solution ◽  
Fluoride Concentration ◽  
Surface Hardness ◽  
Significant Difference ◽  
High Fluoride ◽  
Fluoride Dentifrice ◽  
Mineral Replacement ◽  
Dose Response Effect

The present study aimed to evaluate the effect of fluoride (F) dentifrice with different F concentrations on root dentine de-/remineralization. Ten healthy volunteers took part in this randomized, double-blinded, cross-over, and split-mouth in situ experimental study. During 4 phases of 7 days, they wore a palatal appliance containing 4 bovine dentine blocks (2 sound and 2 with caries) of 4 × 4 × 2 mm. Treatments were performed with silica-based dentifrices containing 0, 700, 1,300, and 5,000 µg F/g (F as NaF). To provide a cariogenic challenge, a 20% sucrose solution was dripped 3 and 8 times daily on the carious-like and sound blocks, respectively. After each experimental phase, the percentage of surface hardness loss (%SHL) or recovery (%SHR) was calculated and the fluoride concentration in the biofilm was determined. The statistical analysis was performed using ANOVA and the Tukey post hoc test with <i>p</i> at 5%. The relationship between variables was analyzed by linear regression. The results showed a lower %SHL when 5,000 µg F/g dentifrice was used but without a statistically significant difference from the conventional one (1,300 µg F/g). Regarding remineralization and F in biofilms, the high-fluoride dentifrice was expressively superior in mineral replacement on the surface and in the F concentration in the biofilms, respectively, compared to the other 3 products (<i>p</i> &#x3c; 0.05). Also, a significant linear fit between mineral loss/gain, F in biofilms, and fluoride concentration in the dentifrices could be observed. In conclusion, a dose-response F effect was observed, and the high-fluoride dentifrice was effective in enhancing root dentine remineralization in this short-term in situ study.

scholarly journals Dose–response effect of fluoride dentifrice on remineralisation and further demineralisation of erosive lesions: A randomised in situ clinical study

2015 ◽  
Vol 43 (7) ◽  
pp. 823-831 ◽  
Author(s):  
J.E. Creeth ◽  
S.A. Kelly ◽  
E.A. Martinez-Mier ◽  
A.T. Hara ◽  
M.L. Bosma ◽  
...  
Keyword(s):  
Clinical Study ◽  
Dose Response ◽  
Response Effect ◽  
Fluoride Dentifrice ◽  
Dose Response Effect

Frequency of Fluoride Dentifrice Use and Caries Lesions Inhibition and Repair

2016 ◽  
Vol 50 (2) ◽  
pp. 133-140 ◽  
Author(s):  
Diego Figueiredo Nóbrega ◽  
Constanza Estefany Fernández ◽  
Altair Antoninha Del Bel Cury ◽  
Livia Maria Andaló Tenuta ◽  
Jaime Aparecido Cury
Keyword(s):  
Sucrose Solution ◽  
Surface Hardness ◽  
Relative Effect ◽  
Double Blind ◽  
In Situ Study ◽  
Enamel Caries ◽  
Fluoride Dentifrice ◽  
The Relationship ◽  
Caries Lesions

The clinical relevance of the frequency of fluoride dentifrice (FD) use on enamel caries is based on evidence. However, the relative effect of FD on reduction of demineralization or enhancement of remineralization is unknown and the effect of frequency on root dentine caries has not been explored. The aim of this double-blind, crossover, in situ study, which was conducted in 4 phases of 14 days each, was to evaluate the relationship between the frequency of FD use and enamel and root dentine de- and remineralization. Eighteen volunteers wore palatal appliances containing enamel and root dentine slabs, either sound or carious. Biofilm accumulation on the slab surface was allowed, and 20% sucrose solution was dripped 3 or 8 times per day on the carious and sound slabs, respectively. Volunteers used FD (1,100 μg F/g) in the frequencies 0 (fluoride-placebo dentifrice), 1, 2 and 3 times per day. The demineralization and remineralization that occurred in sound or carious slabs was estimated by the percentage of surface hardness loss (%SHL) or recovery (%SHR). Loosely (CaF2) and firmly (FAp) bound fluoride concentrations were also determined. The relationship between the variables was analyzed by linear regression. The %SHL, CaF2 and FAp concentrations were a function of the frequency of FD use for enamel and dentine, but the %SHR was a function of the frequency of FD use only for enamel (p < 0.05). The results suggest that demineralization in enamel and root dentine is reduced in proportion to the frequency of FD use, but for remineralization the effect of the frequency of FD use was relevant only to enamel.

In situ Mineral Loss Inhibition by CO2 Laser and Fluoride

2006 ◽  
Vol 85 (7) ◽  
pp. 617-621 ◽  
Author(s):  
L.K.A. Rodrigues ◽  
M. Nobre dos Santos ◽  
J.D.B. Featherstone
Keyword(s):  
Laser Irradiation ◽  
Laser Treatment ◽  
Co2 Laser ◽  
Sucrose Solution ◽  
Treatment Groups ◽  
Enamel Demineralization ◽  
Human Enamel ◽  
Fluoride Dentifrice ◽  
Mineral Loss

Laser and fluoride treatments have been shown to inhibit enamel demineralization in the laboratory. However, the intra-oral effects of this association have not been tested. This study assessed in situ the effect of a Transversely Excited Atmospheric CO2 laser (λ = 9.6 μm) and the use of pressure fluoridated dentifrice on enamel demineralization. During two 14-day phases, 17 volunteers wore palatal appliances containing human enamel slabs assigned to treatment groups, as follows: (1) non-fluoride dentifrice, (2) CO2 laser irradiation plus non-fluoride dentifrice, (3) fluoride dentifrice, and (4) CO2 laser irradiation plus fluoride dentifrice. A 20% sucrose solution was dripped onto the slabs 8 times per day. The specimens treated with laser and/or fluoridated dentifrice presented a significantly lower mineral loss when compared with those from the non-fluoride dentifrice group. The results suggested that CO2 laser treatment of enamel inhibits demineralization in the human mouth, being more effective when associated with fluoride.

scholarly journals Toothpaste Prevents Debonded Brackets on Erosive Enamel

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Érico Luiz Damasceno Barros ◽  
Shelon Cristina Souza Pinto ◽  
Alvaro Henrique Borges ◽  
Mateus Rodrigues Tonetto ◽  
Roger Phillip Ellwood ◽  
...  
Keyword(s):  
Bond Strength ◽  
Clinical Relevance ◽  
Strength Test ◽  
Distilled Water ◽  
Control Groups ◽  
Treatment Groups ◽  
Significant Difference ◽  
High Fluoride ◽  
Fluoride Dentifrice ◽  
Acid Challenge

This study evaluated the effect of high fluoride dentifrice on the bond strength of brackets after erosive challenge. Eighty-four enamel specimens were divided into seven groups(n=12): WN (distilled water/no acid challenge), W3C (distilled water/3 cycles of acid challenge), and W6C (distilled water/6 cycles of acid challenge) were not submitted to dentifrice treatment. Groups RF3C (regular fluoride dentifrice/3 cycles of acid challenge) and RF6C (regular fluoride dentifrice/6 cycles of acid challenge) were treated with dentifrices containing 1450 μg F−/g and HF3C (high fluoride dentifrice/3 cycles of acid challenge) and HF6C (high fluoride dentifrice/6 cycles of acid challenge) were with 5000 μg F−/g. Acid challenges were performed for seven days. After bond strength test, there was no significant difference among groups submitted to 3 cycles of acid challenge(P>0.05). Statistically significant difference was found between the regular and high fluoride dentifrices after 6 cycles of acid challenge (<0.05). Similar areas of adhesive remaining were found among control groups and among groups W6C, RF3C, RF6C, HF3C, and HF6C. The high fluoride dentifrice was able to prevent the reduction of bond strength values of brackets submitted to acid challenge. Clinical relevance: the high fluoride toothpaste prevents debonded brackets on erosive enamel.

Fluoride Release from CaF2 and Enamel Demineralization

2008 ◽  
Vol 87 (11) ◽  
pp. 1032-1036 ◽  
Author(s):  
L.M.A. Tenuta ◽  
R.V. Cerezetti ◽  
A.A. Del Bel Cury ◽  
C.P.M. Tabchoury ◽  
J.A. Cury
Keyword(s):  
Significant Role ◽  
Dose Response ◽  
Calcium Fluoride ◽  
Fluid Phase ◽  
Fluoride Release ◽  
Response Effect ◽  
Enamel Demineralization ◽  
Highly Correlated ◽  
Dose Response Effect

The anticaries effect of professional fluoride (F) application has been attributed to calcium-fluoride-like deposits (CaF2) formed on enamel, but this has not been clearly demonstrated. We hypothesized that CaF2 formed on plaque-free enamel by F application would reduce enamel demineralization due to the increase of F availability in fluid of subsequently formed plaque. We created distinct levels of CaF2 on enamel to evaluate a dose-response effect. Enamel blocks were mounted in contact with a S. mutans test plaque and used in situ by 10 volunteers. F released to the fluid phase of this substrate (“plaque fluid”) was measured before a cariogenic challenge. “Plaque fluid” F concentration was highly correlated to the enamel CaF2 concentration (r = 0.96, p < 0.001) and to consequent enamel demineralization (r = −0.75, p < 0.001). The results suggest that F released to plaque fluid from CaF2 formed on enamel may play a significant role in the anticaries effect of professionally applied F agents.

Calcium Prerinse before Fluoride Rinse Reduces Enamel Demineralization: An in situ Caries Study

2016 ◽  
Vol 50 (4) ◽  
pp. 372-377 ◽  
Author(s):  
João Gabriel S. Souza ◽  
Livia Maria Andaló Tenuta ◽  
Altair Antoninha Del Bel Cury ◽  
Diego Figueiredo Nóbrega ◽  
Renan R. Budin ◽  
...  
Keyword(s):  
Sucrose Solution ◽  
Surface Hardness ◽  
Sodium Lactate ◽  
Negative Control ◽  
Distilled Water ◽  
Double Blind ◽  
Caries Model ◽  
Biofilm Fluid ◽  
Water Rinse

A calcium (Ca) prerinse before a fluoride (F) rinse has been shown to increase oral F levels. We tested the anticaries effect of this combination in a dose-response in situ caries model. In a double-blind, crossover experiment, 10 volunteers carried enamel slabs in palatal appliances for 14 days, during which they rinsed twice/day with one of four rinse combinations: (1) a placebo prerinse (150 mM sodium lactate) followed by a distilled water rinse (negative control); (2) a placebo prerinse followed by a 250 ppm F rinse; (3) a placebo prerinse followed by a 1,000 ppm F rinse, or (4) a Ca prerinse (150 mM Ca, as calcium lactate) followed by a 250 ppm F rinse. Sucrose solution was dripped onto the slabs 8×/day to simulate a high cariogenic challenge. The percent surface hardness loss (%SHL) was significantly lower in the Ca prerinse used with the 250 ppm F rinse group (%SHL = 38.0 ± 21.0) when compared with the F rinse alone (%SHL = 59.5 ± 24.1) and similar to the 1,000 ppm F rinse group (%SHL = 42.0 ± 18.3). Compared with the 250 ppm F rinse, the Ca prerinse increased biofilm fluid F only twice (nonsignificant). However, it greatly increased F in biofilm solids (∼22×). The Ca prerinse had little effect on loosely or firmly bound enamel F. The results showed an increased level of protection against demineralization by the use of a Ca prerinse, which seems to be caused by the enhancement of F concentration in the biofilm.

scholarly journals In situ protocol for the determination of dose-response effect of low-fluoride dentifrices on enamel remineralization

2013 ◽  
Vol 21 (6) ◽  
pp. 525-532 ◽  
Author(s):  
Rebeca Lima AFONSO ◽  
Juliano Pelim PESSAN ◽  
Bruna Babler IGREJA ◽  
Camila Fernandes CANTAGALLO ◽  
Marcelle DANELON ◽  
...  
Keyword(s):  
Dose Response ◽  
Response Effect ◽  
Dose Response Effect

scholarly journals pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization

2008 ◽  
Vol 19 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Celso Silva Queiroz ◽  
Anderson Takeo Hara ◽  
Adriana Franco Paes Leme ◽  
Jaime Aparecido Cury
Keyword(s):  
Dose Response ◽  
Polarized Light ◽  
Positive Control ◽  
Cross Sectional ◽  
Response Effect ◽  
Ph Cycling ◽  
Fluoride Dentifrice ◽  
Dose Response Effect ◽  
Bovine Enamel ◽  
Mineral Loss

Since the currently available pH-cycling models do not differentiate the anti-caries potential of dentifrices with low fluoride (F) concentration, two models were developed and tested in the present. Bovine enamel blocks were subjected to the models and treated with F solutions containing from 70 to 280 mug F/mL in order to validate them in terms of dose-response effect. The models were also tested by evaluating the dentifrices Colgate Baby (500 mug F/g, as a low fluoride dentifrice), Tandy (1,100 mug F/g, as an active F-dentifrice) and Crest (1,100 mug F/g, as positive control). Enamel mineral loss or gain was assessed by surface and cross-sectional microhardness, and lesion depth was analyzed by polarized light microscopy. The pH-cycling models showed F dose-response effect either reducing enamel demineralization or enhancing remineralization. The low F dentifrice presented anti-caries potential, but it was not equivalent to the dentifrices containing 1,100 mug F/g. These data suggest that the models developed in this study were able to evaluate the anti-caries potential of low F dentifrice either on resistance to demineralization or on enhancement of remineralization.

scholarly journals Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jasmin Kirsch ◽  
Matthias Hannig ◽  
Pia Winkel ◽  
Sabine Basche ◽  
Birgit Leis ◽  
...  
Keyword(s):  
Experimental Study ◽  
Fluorescence Microscopy ◽  
Bacterial Adhesion ◽  
Stannous Chloride ◽  
Bacterial Colonization ◽  
Fluoride Concentration ◽  
Pellicle Formation ◽  
Significant Difference ◽  
Bovine Enamel

AbstractThe present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na2PO3F, AmF, SnF2,) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl2 solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF2 and SnCl2 reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na2PO3F showed no significant effect compared to the control. There was no significant difference between AmF, SnF2 and SnCl2. All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF2 appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions’ content.

Effect of 5,000 ppm Fluoride Dentifrice or 1,100 ppm Fluoride Dentifrice Combined with Acidulated Phosphate Fluoride on Caries Lesion Inhibition and Repair

2017 ◽  
Vol 51 (3) ◽  
pp. 179-187 ◽  
Author(s):  
Constanza E. Fernández ◽  
Livia Maria Andaló Tenuta ◽  
Altair Antoninha Del Bel Cury ◽  
Diego Figueiredo Nóbrega ◽  
Jaime Aparecido Cury
Keyword(s):  
Fluoride Concentration ◽  
Negative Control ◽  
Double Blind ◽  
Cross Sectional ◽  
Caries Lesion ◽  
Risk Patients ◽  
High Fluoride ◽  
Fluoride Dentifrice ◽  
The Difference ◽  
Caries Lesions

High fluoride dentifrice (FD; 5,000 ppm F) has been recommended to arrest root dentine lesions and to control enamel caries in high-risk patients. Also, standard FD (1,100 ppm F) in combination with professional fluoride application has been recommended to control dentine caries, but the effect of this combination on enamel has been considered modest. Considering the lack of evaluation comparing the use of 5,000 ppm FD (5,000-FD) versus acidulated phosphate fluoride (APF) application combined with 1,100 ppm FD (1,100-FD) on the inhibition and repair of caries lesions in both enamel and dentine, we conducted this in situ, double-blind, crossover study of 3 phases of 14 days. In each phase, 18 volunteers wore palatal appliances containing enamel and root dentine specimens, either sound or carious, to evaluate the effect of the treatments on the inhibition or repair of caries lesions, respectively. The treatments were non-FD (negative control), 5,000-FD, or 1 APF gel application on dental specimens combined with 1,100-FD used twice per day (APF + 1,100-FD). The reduction of demineralization and enhancement of remineralization were assessed by surface and cross-sectional hardness. Fluoride concentration was determined on dental specimens and on the formed biofilm. For enamel, APF + 1,100-FD and 5,000-FD did not differ regarding the inhibition of demineralization and repair of caries lesions. However, for dentine the difference between these treatments was inconclusive because while APF + 1,100-FD was more effective than 5,000-FD in caries lesion reduction and repair, 5,000-FD was more effective than APF + 1,100-FD in the reduction of surface demineralization. Therefore, the findings show that the combination of APF + 1,100-FD is as effective as 5,000-FD in enamel inhibition of demineralization and enhancement of remineralization.

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