The Role of Hippocampal Neurogenesis in ANT-DBS for LiCl-Pilocarpine-Induced Epileptic Rats

2020 ◽  
pp. 1-10
Author(s):  
Weiting Tang ◽  
Xinghui He ◽  
Li Feng ◽  
Dingyang Liu ◽  
Zhuanyi Yang ◽  
...  
Keyword(s):  
Elevated Plus Maze ◽  
Hippocampal Neurogenesis ◽  
Male Rats ◽  
Anterior Thalamic Nucleus ◽  
Chronic Stage ◽  
Plus Maze ◽  
Spontaneous Recurrent Seizures ◽  
Pilocarpine Injection ◽  
Deep Brain

<b><i>Purpose:</i></b> Abnormal neurogenesis in the hippocampus after status epilepticus (SE) has been suggested as a key pathogeny of temporal lobe epilepsy. This study aimed to investigate the effect of deep brain stimulation of the anterior thalamic nucleus (ANT-DBS) on hippocampal neurogenesis in LiCl-pilocarpine-induced epileptic rats and to analyze its relationship with postoperative spontaneous recurrent seizures (SRS) and anxiety. <b><i>Method:</i></b> SE was induced by a systemic LiCl-pilocarpine injection in adult male rats. Rats in the DBS group underwent ANT-DBS immediately after successful SE induction. SRS was only recorded during the chronic stage. An elevated plus maze was used to evaluate the level of anxiety in rats 7, 28, and 60 days after SE onset. After the elevated plus-maze experiment, rats were sacrificed under anesthesia in order to evaluate hippocampal neurogenesis. Doublecortin (DCX) was used as a marker for neurogenesis. <b><i>Results:</i></b> During the chronic stage, SRS in rats in the DBS group were significantly decreased. The level of anxiety was increased significantly in rats in the DBS group 28 days after SE, while no significant differences in anxiety levels were found 7 and 60 days after SE. The number of DCX-positive cells in the hippocampus was significantly increased 7 days after SE and was significantly decreased 60 days after SE in all rats in which SE was induced. However, the number of DCX-positive cells in the DBS group was significantly lower than that in the other groups 28 days after SE. <b><i>Conclusions:</i></b> ANT-DBS may suppress SRS and increase the postoperative anxiety of epileptic rats by influencing hippocampal neurogenesis.

The role of vision and proprioception in the aversion of rats to the open arms of an elevated plus-maze

2002 ◽  
Vol 60 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Juan Carlos Martı́nez ◽  
Fernando Cardenas ◽  
Marisol Lamprea ◽  
Silvio Morato
Keyword(s):  
Elevated Plus Maze ◽  
Plus Maze

scholarly journals Aqueous Extract of Semen Ziziphi Spinosae Exerts Anxiolytic Effects during Nicotine Withdrawal via Improvement of Amygdaloid CRF/CRF1R Signaling

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Changhong Gu ◽  
ZhengLin Zhao ◽  
Xiaodong Zhu ◽  
Tong Wu ◽  
Bong Hyo Lee ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Elevated Plus Maze ◽  
Plasma Corticosterone ◽  
Nicotine Withdrawal ◽  
Corticotropin Releasing Factor ◽  
Central Nucleus ◽  
Male Rats ◽  
General Anxiety ◽  
Plus Maze

Anxiety during nicotine withdrawal (NicW) is a key risk factor for smoking relapse. Semen Ziziphi Spinosae (SZS), which is a prototypical hypnotic-sedative herb in Oriental medicine, has been clinically used to treat insomnia and general anxiety disorders for thousands of years. Thus, the present study evaluated the effects of the aqueous extract of SZS (AESZS) on NicW-induced anxiety in male rats that received subcutaneous administrations of nicotine (Nic) (0.4 mg/kg, twice a day) for 7 d followed by 4 d of withdrawal. During NicW, the rats received four intragastric treatments of AESZS (60 mg/kg/d or 180 mg/kg/d). AESZS dose-dependently attenuated NicW-induced anxiety-like behaviors in the elevated plus maze (EPM) tests and 180 mg/kg/d AESZS inhibited NicW-induced increases in plasma corticosterone. Additionally, the protein and mRNA expressions of corticotropin-releasing factor (CRF) and CRF type 1 receptor (CRF1R) increased in the central nucleus of the amygdala (CeA) during NicW, but these changes were suppressed by 180 mg/kg/d AESZS. A post-AESZS infusion of CRF into the CeA abolished the attenuation of anxiety by AESZS and 180 mg/kg/d AESZS suppressed NicW-induced increases in norepinephrine and 3-methoxy-4-hydroxy-phenylglycol levels in the CeA. The present results suggest that AESZS ameliorated NicW-induced anxiety via improvements in CRF/CRF1R and noradrenergic signaling in the CeA.

Effect of Surfagon on Open Field and Elevated Plus Maze Behavior of Gonadectomized and Non-Gonadectomized Male Rats

2019 ◽  
Vol 168 (1) ◽  
pp. 52-54
Author(s):  
O. O. Masalova ◽  
S. B. Kazakova ◽  
T. N. Savateeva-Lyubimova ◽  
K. V. Sivak ◽  
N. S. Sapronov ◽  
...  
Keyword(s):  
Open Field ◽  
Elevated Plus Maze ◽  
Male Rats ◽  
Plus Maze

scholarly journals Role of 5-HT1A Receptor in the Anxiolytic-Relaxant Effects of Bergamot Essential Oil in Rodent

2020 ◽  
Vol 21 (7) ◽  
pp. 2597 ◽  
Author(s):  
Laura Rombolà ◽  
Damiana Scuteri ◽  
Chizuko Watanabe ◽  
Shinobu Sakurada ◽  
Kengo Hamamura ◽  
...  
Keyword(s):  
Essential Oil ◽  
Elevated Plus Maze ◽  
Relaxant Effect ◽  
Serotonergic Receptors ◽  
Selective Agonist ◽  
Plus Maze ◽  
Way 100635 ◽  
Pharmacological Data ◽  
Citrus Bergamia

The essential oil obtained by the fresh fruit of Citrus bergamia Risso et Poiteau is used worldwide in aromatherapy to reduce pain, facilitate sleep induction, and/or minimize the effects of stress-induced anxiety. Preclinical pharmacological data demonstrate that bergamot essential oil (BEO) modulates specific neurotransmissions and shows an anxiolytic-relaxant effect not superimposable to that of the benzodiazepine diazepam, suggesting that neurotransmissions, other than GABAergic, could be involved. Several studies on essential oils indicate a role for serotonergic (5-HT) neurotransmission in anxiety. Interestingly, among serotonergic receptors, the 5-HT1A subtype seems to play a key role in the control of anxiety. Here, we report that modulation of the 5-HT1A receptor by selective agonist ((±)8-OH-DPAT) or antagonist (WAY-100635) may influence some of the anxiolytic-relaxant effects of BEO in Open Field and Elevated Plus Maze tests.

Role of neurogenesis in distinguishing relevant from irrelevant memories

2011 ◽  
Vol 26 (S2) ◽  
pp. 655-655
Author(s):  
M. Moreno ◽  
E. Glennon ◽  
L. Thiru ◽  
C. Sexton ◽  
J.D. Coplan ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Spatial Segregation ◽  
Hippocampal Neurogenesis ◽  
Male Rats ◽  
Antidepressant Effect ◽  
Spatial Cues ◽  
Adult Rats ◽  
Immature Neuron ◽  
Stimulation Of

BackgroundIn this study we examine potential mechanisms by which the stimulation of hippocampal neurogenesis may generate an antidepressant effect.MethodsStudy-1: Adult male rats (N = 24) were trained to segregate relevant from irrelevant spatial cues (spatial segregation); tested on this task four and 8-weeks late; then exposed (on week 8) to a modified version of the task that conflicted with the memory of the initially learned experience (mnemonic segregation); and then euthanized to detect hippocampal neurogenesis. Study-2: Adult rats (N = 24) were trained in the spatial segregation task; three-days later, half were re-tested on the same task and half the tested on the modified task (mnemonic segregation); and euthanized immediately to detect neurons that were synaptically active during task performance.ResultsStudy-1: Good performers on the modified task (mnemonic segregation) had significantly greater rates of hippocampal neurogenesis, but the increase was only in immature neurons and not in new neurons that had completed maturation. Performance on spatial segregation task was unrelated to proficiency in mnemonic segregation or rates of neurogenesis. Study-2: Performance on the mnemonic segregation unrelated to neurogenesis rates, but inversely correlated to synaptic activation of mature hippocampal neurons, which in turn inversely correlated with immature neuron rates.ConclusionTaken together, the data suggests that neurogenesis facilitates detection of subtle changes to experiences established over several weeks (not days); this occurs prior to forming synapses; and maybe associated with suppression of mature hippocampal neurons that presumably mediate older, interfering, experiences.

Nicotine-induced anxiogenic-like behaviours of rats in the elevated plus-maze: possible role of NMDA receptors of the central amygdala

2011 ◽  
Vol 26 (4) ◽  
pp. 555-563 ◽  
Author(s):  
Mohammad Reza Zarrindast ◽  
Arash Aghamohammadi-Sereshki ◽  
Ameneh Rezayof ◽  
Parvin Rostami
Keyword(s):  
Locomotor Activity ◽  
Nmda Receptor ◽  
Nmda Receptors ◽  
Elevated Plus Maze ◽  
Nmda Receptor Antagonist ◽  
Central Amygdala ◽  
Receptor System ◽  
Plus Maze ◽  
Male Wistar Rats

The objective of the present study was to investigate the possible role of the N-methyl-D-aspartate (NMDA) receptor system of the central amygdala (CeA) in the anxiogenic-like effect of nicotine. Male Wistar rats with cannulas aimed to the CeA were submitted to the elevated plus-maze (EPM). Intraperitoneal (i.p.) injections of nicotine (0.6 and 0.8 mg/kg) decreased percentage open arm time spent (%OAT) and percentage open arm entries (%OAE), but not locomotor activity, indicating an anxiogenic-like response. Bilateral intra-CeA microinjection of NMDA (0.005–0.1 μ g/rat) decreased %OAT, but not %OAE and locomotor activity. Moreover, intra-CeA microinjection of NMDA (0.05 μ g) with an ineffective dose of nicotine (0.4 mg/kg, i.p.) reduced %OAT and %OAE without effect on locomotor activity. On the other hand, intra-CeA microinjection of the NMDA receptor antagonist D-AP5 (0.05–0.5 μ g/rat) increased both %OAT and %OAE, showing an anxiolytic-like effect of the drug. Co-administration of the same doses of D-AP5 with nicotine (0.6 mg/kg, i.p.) increased %OAT and %OAE, but not locomotor activity. Intra-CeA microinjection of D-AP5 reversed the response induced by NMDA (0.1 μ g/rat) in the EPM. The results may support the possible involvement of glutamate transmission, through NMDA receptors of central amygdala in the anxiogenic-like effect of nicotine in the EPM task.

Anxiolytic effect of carbamazepine in the elevated plus-maze: possible role of adenosine

1992 ◽  
Vol 106 (1) ◽  
pp. 85-89 ◽  
Author(s):  
H. Zangrossi ◽  
J. R. Leite ◽  
F. G. Graeff
Keyword(s):  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Plus Maze

Age and sex affect spatial and emotional behaviors in rats: The role of repeated elevated plus maze test

Neuroscience ◽  
2012 ◽  
Vol 227 ◽  
pp. 1-9 ◽  
Author(s):  
M. Belviranli ◽  
K.E.N. Atalik ◽  
N. Okudan ◽  
H. Gökbel
Keyword(s):  
Elevated Plus Maze ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Age And Sex
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