MiR-204-5p Inhibits Transforming Growth Factor-β1-Induced Proliferation and Extracellular Matrix Production of Airway Smooth Muscle Cells by Regulating Six1 in Asthma

2020 ◽  
Vol 181 (4) ◽  
pp. 239-248 ◽  
Author(s):  
Zhaochuan Yang ◽  
Zhenghai Qu ◽  
Mingji Yi ◽  
Zhidong Lv ◽  
Yanxia Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Airway Smooth Muscle Cells ◽  
Extracellular Matrix Production ◽  
Matrix Production

Noncanonical WNT‐5A signaling regulates TGF‐β‐induced extracellular matrix production by airway smooth muscle cells

2012 ◽  
Vol 27 (4) ◽  
pp. 1631-1643 ◽  
Author(s):  
Kuldeep Kumawat ◽  
Mark H. Menzen ◽  
I. Sophie T. Bos ◽  
Hoeke A. Baarsma ◽  
Pieter Borger ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells ◽  
Extracellular Matrix Production ◽  
Matrix Production

Hyperin Ameliorates Proliferation, Migration, and Extracellular Matrix Formation in Airway Smooth Muscle Cells by Inhibiting Transforming Growth Factor-β1-Induced Nuclear Factor-κB Activation

2020 ◽  
Vol 19 (2) ◽  
pp. 222-227
Author(s):  
Nan Zhang ◽  
Tingting Zhao ◽  
Meirong Bi ◽  
Xuejia He ◽  
Yamin Zhang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Nuclear Factor Kappa B ◽  
Transforming Growth Factor ◽  
Muscle Cells ◽  
Transforming Growth Factor Β1 ◽  
Airway Smooth Muscle Cells ◽  
Nuclear Factor

We have explored the role of hyperin remodeling of airway smooth muscle cells during asthma. To this end, airway smooth muscle cells were isolated from tracheae and bronchi of mice, treated with transforming growth factor-β1 and increasing concentrations of hyperin followed by analysis of cell viability, proliferation, and migration. The levels of extracellular matrix formation were evaluated by analysis of fibronectin and type I collagen. Western blot analysis was used to assess the function of the downstream pathway of nuclear factor-kappa B. Transforming growth factor-β1 treatment led to a dose-dependent increase in type I collagen and fibronectin that was reversed by hyperin. Transforming growth factor-β1 promoted activation of nuclear factor-kappa B pathway with reduced IκBα and enhanced phospho (p)-p65 and p-IκBα. However, hyperin treatment upregulated IκBα and downregulated p-p65/p-IκBα to inactivate NF-κB pathway. In conclusion, hyperin ameliorates proliferation, migration, and extracellular matrix formation in airway smooth muscle cells by inhibiting transforming growth factor-β1-induced nuclear factor-kappa B activation suggesting potential prevention of airway remodeling during asthma.

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