Frameless Stereotactic Targeting of the Cerebellar Dentate Nucleus in Nonhuman Primates: Translatable Model for the Surgical Delivery of Gene Therapy

2019 ◽  
Vol 97 (5-6) ◽  
pp. 293-302
Author(s):  
Jeremy S. Wetzel ◽  
Nathan Hardcastle ◽  
Muhibullah S. Tora ◽  
Thais Federici ◽  
Stephen Frey ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Nonhuman Primates ◽  
Dentate Nucleus ◽  
Cerebellar Dentate Nucleus

Cerebellar Dentate Nucleus in Alzheimer’s Disease with Myoclonus

1999 ◽  
Vol 10 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Yuken Fukutani ◽  
NigelJ. Cairns ◽  
IanP. Everall ◽  
Andrew Chadwick ◽  
Kiminori Isaki ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Dentate Nucleus ◽  
Cerebellar Dentate Nucleus

Cerebellar dentate nucleus functional connectivity with cerebral cortex in Alzheimer's disease and memory: a seed-based approach

2020 ◽  
Vol 89 ◽  
pp. 32-40 ◽  
Author(s):  
Giusy Olivito ◽  
Laura Serra ◽  
Camillo Marra ◽  
Carlotta Di Domenico ◽  
Carlo Caltagirone ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cerebral Cortex ◽  
Functional Connectivity ◽  
Dentate Nucleus ◽  
Cerebellar Dentate Nucleus

scholarly journals A Single Dose of CD117 Antibody Drug Conjugate Enables Hematopoietic Stem Cell Based Gene Therapy in Nonhuman Primates

2020 ◽  
Vol 26 (3) ◽  
pp. S6
Author(s):  
Naoya Uchida ◽  
John F. Tisdale ◽  
Robert E. Donahue ◽  
Bradley R. Pearse ◽  
Sean M. McDonough ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Stem Cell ◽  
Single Dose ◽  
Hematopoietic Stem Cell ◽  
Nonhuman Primates ◽  
Antibody Drug Conjugate ◽  
Hematopoietic Stem ◽  
Drug Conjugate ◽  
Antibody Drug

scholarly journals Gene Therapy for Attenuating Cardiac Allograft Arteriopathy Using Ex Vivo E2F Decoy Transfection by HVJ-AVE–Liposome Method in Mice and Nonhuman Primates

2000 ◽  
Vol 87 (11) ◽  
pp. 1063-1068 ◽  
Author(s):  
Motohiro Kawauchi ◽  
Jun-ichi Suzuki ◽  
Ryuichi Morishita ◽  
Yuko Wada ◽  
Atsushi Izawa ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Nonhuman Primates ◽  
Ex Vivo ◽  
Cardiac Allograft

Retroviral transduction efficiency of G-CSF+SCF–mobilized peripheral blood CD34+ cells is superior to G-CSF or G-CSF+Flt3-L–mobilized cells in nonhuman primates

Blood ◽  
2003 ◽  
Vol 101 (6) ◽  
pp. 2199-2205 ◽  
Author(s):  
Peiman Hematti ◽  
Stephanie E. Sellers ◽  
Brian A. Agricola ◽  
Mark E. Metzger ◽  
Robert E. Donahue ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Peripheral Blood ◽  
Nonhuman Primates ◽  
Transduction Efficiency ◽  
Target Cells ◽  
Hematopoietic Stem ◽  
Retroviral Transduction ◽  
Peripheral Blood Cd34 ◽  
Cd34 Cells ◽  
Clinical Gene Therapy

Gene transfer experiments in nonhuman primates have been shown to be predictive of success in human clinical gene therapy trials. In most nonhuman primate studies, hematopoietic stem cells (HSCs) collected from the peripheral blood or bone marrow after administration of granulocyte colony-stimulating factor (G-CSF) + stem cell factor (SCF) have been used as targets, but this cytokine combination is not generally available for clinical use, and the optimum target cell population has not been systematically studied. In our current study we tested the retroviral transduction efficiency of rhesus macaque peripheral blood CD34+ cells collected after administration of different cytokine mobilization regimens, directly comparing G-CSF+SCF versus G-CSF alone or G-CSF+Flt3-L in competitive repopulation assays. Vector supernatant was added daily for 96 hours in the presence of stimulatory cytokines. The transduction efficiency of HSCs as assessed by in vitro colony-forming assays was equivalent in all 5 animals tested, but the in vivo levels of mononuclear cell and granulocyte marking was higher at all time points derived from target CD34+ cells collected after G-CSF+SCF mobilization compared with target cells collected after G-CSF (n = 3) or G-CSF+Flt3-L (n = 2) mobilization. In 3 of the animals long-term marking levels of 5% to 25% were achieved, but originating only from the G-CSF+SCF–mobilized target cells. Transduction efficiency of HSCs collected by different mobilization regimens can vary significantly and is superior with G-CSF+SCF administration. The difference in transduction efficiency of HSCs collected from different sources should be considered whenever planning clinical gene therapy trials and should preferably be tested directly in comparative studies.

Spinal Projections of the Cat Parvicellular Red Nucleus

2002 ◽  
Vol 87 (1) ◽  
pp. 453-468 ◽  
Author(s):  
Milton Pong ◽  
Kris M. Horn ◽  
Alan R. Gibson
Keyword(s):  
Spinal Cord ◽  
Dentate Nucleus ◽  
Inferior Olive ◽  
Red Nucleus ◽  
Interpositus Nucleus ◽  
Limb Musculature ◽  
Caudal Portion ◽  
Cerebellar Interpositus Nucleus ◽  
Cerebellar Dentate Nucleus ◽  
Rostral Portion

Traditionally, the red nucleus of the cat is divided into two parts: a large-celled, magnocellular, division (RNm) and a small-celled, parvicellular, division (RNp). The RNm projects to the spinal cord and receives input from the cerebellar interpositus nucleus. The RNp projects to the inferior olive and receives input from the cerebellar dentate nucleus. In this report, we reexamine the connections of the red nucleus using the bidirectional tracer wheat germ agglutinin-horseradish peroxidase (WGA-HRP). Our findings demonstrate that the cat RNp has a large caudal and lateral region that projects to contralateral spinal cord and not to the inferior olive. The spinally projecting region of RNp receives input from the dentate nucleus and a lateral segment of anterior interpositus. Cervical projections from the red nucleus show a topography with the rostral portion of RNp favoring upper segments and the caudal portion of RNm favoring lower segments. The results show that dentate output can influence spinal activity without passing through the cerebral cortex. For the control of movements such as reaching and grasping, we suggest that RNp and dentate focus on the control of proximal limb musculature, whereas RNm and the anterior interpositus focus on the control of distal limb musculature. We also suggest that other species are likely to have a small-celled area of red nucleus projecting to the spinal cord.

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