Blue-Light Fundus Autofluorescence Imaging following Ruthenium-106 Brachytherapy for Choroidal Melanoma

2020 ◽  
Vol 243 (4) ◽  
pp. 303-315 ◽  
Author(s):  
Almut Bindewald-Wittich ◽  
Tomasz Swenshon ◽  
Eva Carasco ◽  
Jens Dreyhaupt ◽  
Gregor D. Willerding
Keyword(s):  
Blue Light ◽  
Fundus Autofluorescence ◽  
Choroidal Melanoma ◽  
Autofluorescence Imaging ◽  
Ruthenium 106 Brachytherapy

Current Methods for the Diagnosis and Treatment of Choroidal Melanoma

2012 ◽  
Vol 05 (01) ◽  
pp. 62 ◽  
Author(s):  
Vasileios Petousis ◽  
Paul T Finger ◽  
◽  
Keyword(s):  
Computed Tomography ◽  
Radiation Therapy ◽  
Fundus Autofluorescence ◽  
Choroidal Melanoma ◽  
Diagnostic Techniques ◽  
Diagnosis And Treatment ◽  
Autofluorescence Imaging ◽  
Tumor Biopsy ◽  
Positron Emission ◽  
Cancer Specialists

Choroidal melanoma is the most common primary intraocular malignancy. Diagnosis is performed by clinical evaluation: eye cancer specialists currently use multiple diagnostic techniques, including ophthalmoscopy, fundus autofluorescence imaging (FAF), fluorescein angiography (FA), and indocyanine green angiography (ICG), as well as optical coherence tomography (OCT). Tumor biopsy has recently become popular due to the availability of diagnostic cytogenetic features, touted as biomarkers for metastasis. Standard treatments include observation (select small melanomas and indeterminate tumors), radiation therapy (plaque, proton beam), eye wall resection, and removal of the eye. Of these, ophthalmic plaque radiation therapy has become the most common and widely available conservative treatment. Successful initial treatment offers the best chance to prevent subsequent metastasis. Metastatic choroidal melanoma is typically diagnosed by combinations of physical examination, hematologic surveys, abdominal radiographic imaging (ultrasound, magnetic resonance imaging, computed tomography), and positron emission tomography–computed tomography (PET/CT). Most often initially found in the liver, metastatic choroidal melanoma carries an unfavorable prognosis for life. Herein, we report on currently available methods of diagnosis and treatment for choroidal melanoma.

scholarly journals Clinical Phenotype of PDE6B-Associated Retinitis Pigmentosa

2021 ◽  
Vol 22 (5) ◽  
pp. 2374
Author(s):  
Laura Kuehlewein ◽  
Ditta Zobor ◽  
Katarina Stingl ◽  
Melanie Kempf ◽  
Fadi Nasser ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Genetic Testing ◽  
Retinitis Pigmentosa ◽  
Fundus Autofluorescence ◽  
Retinal Disease ◽  
New Drugs ◽  
Autofluorescence Imaging ◽  
Full Field ◽  
Tertiary Referral Center ◽  
The Right

In this retrospective, longitudinal, observational cohort study, we investigated the phenotypic and genotypic features of retinitis pigmentosa associated with variants in the PDE6B gene. Patients underwent clinical examination and genetic testing at a single tertiary referral center, including best-corrected visual acuity (BCVA), kinetic visual field (VF), full-field electroretinography, full-field stimulus threshold, spectral domain optical coherence tomography, and fundus autofluorescence imaging. The genetic testing comprised candidate gene sequencing, inherited retinal disease gene panel sequencing, whole-genome sequencing, and testing for familial variants by Sanger sequencing. Twenty-four patients with mutations in PDE6B from 21 families were included in the study (mean age at the first visit: 32.1 ± 13.5 years). The majority of variants were putative splicing defects (8/23) and missense (7/23) mutations. Seventy-nine percent (38/48) of eyes had no visual acuity impairment at the first visit. Visual acuity impairment was mild in 4% (2/48), moderate in 13% (6/48), and severe in 4% (2/48). BCVA was symmetrical in the right and left eyes. The kinetic VF measurements were highly symmetrical in the right and left eyes, as was the horizontal ellipsoid zone (EZ) width. Regarding the genetic findings, 43% of the PDE6B variants found in our patients were novel. Thus, this study contributed substantially to the PDE6B mutation spectrum. The visual acuity impairment was mild in 83% of eyes, providing a window of opportunity for investigational new drugs. The EZ width was reduced in all patients and was highly symmetric between the eyes, making it a promising outcome measure. We expect these findings to have implications on the design of future PDE6B-related retinitis pigmentosa (RP) clinical trials.

scholarly journals Structural evaluation in inherited retinal diseases

2021 ◽  
pp. bjophthalmol-2021-319228
Author(s):  
Malena Daich Varela ◽  
Burak Esener ◽  
Shaima A Hashem ◽  
Thales Antonio Cabral de Guimaraes ◽  
Michalis Georgiou ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Fundus Autofluorescence ◽  
Standard Of Care ◽  
Laser Speckle ◽  
Fundus Photography ◽  
Laser Speckle Flowgraphy ◽  
Retinal Diseases ◽  
Autofluorescence Imaging ◽  
Structural Evaluation ◽  
Fundus Imaging

Ophthalmic genetics is a field that has been rapidly evolving over the last decade, mainly due to the flourishing of translational medicine for inherited retinal diseases (IRD). In this review, we will address the different methods by which retinal structure can be objectively and accurately assessed in IRD. We review standard-of-care imaging for these patients: colour fundus photography, fundus autofluorescence imaging and optical coherence tomography (OCT), as well as higher-resolution and/or newer technologies including OCT angiography, adaptive optics imaging, fundus imaging using a range of wavelengths, magnetic resonance imaging, laser speckle flowgraphy and retinal oximetry, illustrating their utility using paradigm genotypes with on-going therapeutic efforts/trials.

scholarly journals Fundus autofluorescence imaging of macular star

2009 ◽  
Vol 87 (6) ◽  
pp. 690-691 ◽  
Author(s):  
Ali Ayata ◽  
Melih Ünal ◽  
Dilaver Erşanlı ◽  
Sinan Tatlıpınar
Keyword(s):  
Fundus Autofluorescence ◽  
Autofluorescence Imaging ◽  
Macular Star

Wide-Field Fundus Autofluorescence Imaging of Retinitis Pigmentosa

Ophthalmology ◽  
2013 ◽  
Vol 120 (9) ◽  
pp. 1827-1834 ◽  
Author(s):  
Akio Oishi ◽  
Ken Ogino ◽  
Yukiko Makiyama ◽  
Satoko Nakagawa ◽  
Masafumi Kurimoto ◽  
...  
Keyword(s):  
Retinitis Pigmentosa ◽  
Fundus Autofluorescence ◽  
Wide Field ◽  
Autofluorescence Imaging

scholarly journals Comparison of short-wavelength blue-light autofluorescence and conventional blue-light autofluorescence in geographic atrophy

2018 ◽  
Vol 103 (5) ◽  
pp. 610-616 ◽  
Author(s):  
Enrico Borrelli ◽  
Muneeswar Gupta Nittala ◽  
Nizar Saleh Abdelfattah ◽  
Jianqin Lei ◽  
Amir H Hariri ◽  
...  
Keyword(s):  
Blue Light ◽  
Imaging Modality ◽  
Fundus Autofluorescence ◽  
Intraclass Correlation ◽  
Geographic Atrophy ◽  
Area Measurement ◽  
Excitation Wavelength ◽  
Lesion Area ◽  
Significant Difference ◽  
The Individual

Background/aimsTo systematically compare the intermodality and inter-reader agreement for two blue-light confocal fundus autofluorescence (FAF) systems.MethodsThirty eyes (21 patients) with a diagnosis of geographic atrophy (GA) were enrolled. Eyes were imaged using two confocal blue-light FAF devices: (1) Spectralis device with a 488 nm excitation wavelength (488-FAF); (2) EIDON device with 450 nm excitation wavelength and the capability for ‘colour’ FAF imaging including both the individual red and green components of the emission spectrum. Furthermore, a third imaging modality (450-RF image) isolating and highlighting the red emission fluorescence component (REFC) was obtained and graded. Each image was graded by two readers to assess inter-reader variability and a single image for each modality was used to assess the intermodality variability.ResultsThe 95% coefficient of repeatability (1.35 mm2 for the 488-FAF-based grading, 8.13 mm2 for the 450-FAF-based grading and 1.08 mm2 for the 450-RF-based grading), the coefficient of variation (1.11 for 488-FAF, 2.05 for 450-FAF, 0.92 for 450-RF) and the intraclass correlation coefficient (0.994 for 488-FAF, 0.711 for 450-FAF, 0.997 for 450-RF) indicated that 450-FAF-based and 450-RF-based grading have the lowest and highest inter-reader agreements, respectively. The GA area was larger for 488-FAF images (median (IQR) 2.1 mm2 (0.8–6.4 mm2)) than for 450-FAF images (median (IQR) 1.0 mm2 (0.3–4.3 mm2); p<0.0001). There was no significant difference in lesion area measurement between 488-FAF-based and 450-RF-based grading (median (IQR) 2.6 mm2 (0.8–6.8 mm2); p=1.0).ConclusionThe isolation of the REFC from the 450-FAF images allowed for a reproducible quantification of GA. This assessment had good comparability with that obtained with 488-FAF images.

scholarly journals Evaluation of fundus autofluorescence ımaging of diabetic patients without retinopathy

2019 ◽  
Vol 82 (5) ◽  
Author(s):  
Sedat Özmen ◽  
Sümeyra Ağca ◽  
Emine Doğan ◽  
Nilgün Özkan Aksoy ◽  
Burçin Çakır ◽  
...  
Keyword(s):  
Fundus Autofluorescence ◽  
Diabetic Patients ◽  
Autofluorescence Imaging

scholarly journals Clinical and Haplotypic Variability of Slovenian USH2A Patients Homozygous for the c. 11864G>A Nonsense Mutation

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1015 ◽  
Author(s):  
Andrej Zupan ◽  
Ana Fakin ◽  
Saba Battelino ◽  
Martina Jarc-Vidmar ◽  
Marko Hawlina ◽  
...  
Keyword(s):  
Disease Severity ◽  
Nonsense Mutation ◽  
Age Of Onset ◽  
Phenotypic Variability ◽  
Fundus Autofluorescence ◽  
Usher Syndrome ◽  
Test Methods ◽  
Rapid Screening ◽  
Autofluorescence Imaging ◽  
Phenotype Analysis

Purpose: to determine a detailed clinical and haplotypic variability of the Slovenian USH2A patients with homozygous c.11864G>A (p.Trp3955Ter) nonsense mutation and to develop sensitive, accurate and rapid screening test. Methods: Ten unrelated homozygous patients with detailed ophthalmological exam were included in our study. The High-Resolution Melting (HRM) method was developed for fast and reliable detection of the c.11864G>A mutation. Results: The c.11864G>A mutation represents the vast majority of pathogenic alleles in Slovenian USH2A-Usher syndrome population (84%). The median age of onset of nyctalopia was 16 years and all patients younger than 40 years had hyperautofluorescent rings on fundus autofluorescence imaging. The Kaplan Meier survival analysis showed a decline of central vision after the age of 40, with 50% patients reaching visual acuity (VA) ≤ 0.05 at the average age of 66 years visual field diameter less than 20° at the average age of 59 years. There was a relatively large phenotypic variability in the retinal and audiological phenotype. Analysis of the p.Trp3955Ter-homozygous patients revealed four different haplotypes, with the frequency of the most common haplotype ~65%. Disease severity did not correlate with the haplotype. Conclusions: According to the natural history of homozygous p.Trp3955Ter patients any therapy aimed to slow disease progression in these patients would be best started before the age of 40. Phenotypic variability suggests the presence of cis and/or trans factors outside the USH2A gene that are able to affect disease severity. High frequency of p.Trp3955Ter mutation in Slovenian USH2A gene pool appears to be initiated from different unrelated founders because of migrations from neighboring populations. The mutation on haplotype 2 seems to be the major founder allele.

Fundus Autofluorescence Imaging

2005 ◽  
pp. 19-34
Author(s):  
Almut Bindewald ◽  
Felix Roth ◽  
Steffen Schmitz-Valckenberg ◽  
Hendrik P.N. Scholl ◽  
Frank G. Holz
Keyword(s):  
Fundus Autofluorescence ◽  
Autofluorescence Imaging
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