Preparation and Comparison of Effects of Different Herbal Oil Ointments as Wound-Healing Agents

2019 ◽  
Vol 207 (3-4) ◽  
pp. 177-186 ◽  
Author(s):  
Alireza Valizadeh ◽  
Mahdieh Shirzad ◽  
Mohammad Reza Pourmand ◽  
Maryam Farahmandfar ◽  
Hassan Sereshti ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Dorsal Surface ◽  
Inflammatory Cells ◽  
Hair Follicles ◽  
Sesame Oil ◽  
Full Thickness ◽  
Olive Oils ◽  
Complex Process ◽  
And Control

Wound healing is a complex process and some agents have been reported to accelerate it. The aim of this study was to evaluate the healing effect of Eucerin-based ointments of lemon, sesame and olive oils on infected full-thickness wounds in rats. Wounds were created on the dorsal surface of Male Albino Wistar rats (n = 12). Wounds were treated with an Eucerin-based ointment containing either of lemon, sesame or olive oils (33% w/w) twice a day for 14 days. Histopathology results showed that contraction of wounds treated with lemon and sesame oils was higher than in the olive oil and control groups on days 10 and 14. In the lemon- and sesame-oil treated groups, on day 14, 50% of rat lesions were completely healed. Total number of inflammatory cells in lemon oil treatment group was significantly smaller than that of others on day 14 (p < 0.001). Also, thickness of the epidermal layer and rejuvenation of the hair follicles and other skin appendages was normal in lemon and sesame oil treated groups. The lemon and sesame oil ointments accelerated the healing process of wounds in macroscopic, morphological and morphmetrical analyses. Therefore, lemon and sesame oil ointments could be considered as alternative dressings for infected full-thickness wounds because of improved wound healing characteristics.

scholarly journals Chitosan/alginate Hydrogel Dressing Loaded FGF/VE-cadherin: To Accelerate Full-thickness Skin Regeneration and More Normal Skin Repairs

2021 ◽  
Author(s):  
Lai Wei ◽  
Jianying Tan ◽  
Li Li ◽  
Sainan Liu ◽  
Huanran Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rheological Properties ◽  
Normal Skin ◽  
Healing Process ◽  
Antibacterial Properties ◽  
Hair Follicles ◽  
Skin Regeneration ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

The process of full-thickness skin regeneration is complex and has many parameters involved, which makes it difficult to use a single dressing to meet the various requirements of the complete regeneration at the same time. Therefore, developing hydrogel dressings with multifunction, including tunable rheological properties and aperture, hemostatic, antibacterial and super cytocompatibility, is a desirable candidate in wound healing. In this study, a series of complex hydrogels were developed via the hydrogen bond and covalent bond between chitosan (CS) and alginate (SA). These hydrogels exhibited suitable pore size and tunable rheological properties for cell adhesion. Chitosan endowed hemostatic, antibacterial properties and great cytocompatibility and thus solved two primary problems in the early stage of the wound healing process. Moreover, the sustained cytocompatibility of the hydrogels was further investigated after adding FGF and VE-cadherin via the co-culture of L929 and EC for 12 days. The confocal 3D fluorescent images showed that the cells were spherical and tended to form multicellular spheroids, which distributed in about 40-60&mu;m thick hydrogels. Furthermore, the hydrogel dressings significantly accelerate defected skin turn to normal skin with proper epithelial thickness and new blood vessels and hair follicles through the histological analysis of in vivo wound healing. The findings mentioned above demonstrated that the CS/SA hydrogels with growth factors have tremendous potential as multifunctional hydrogel dressings for full-thickness skin regeneration incorporated with hemostatic, antibacterial, sustained cytocompatibility for 3D cell culture and normal skin repairing.

scholarly journals Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2554
Author(s):  
Marek Konop ◽  
Anna K. Laskowska ◽  
Mateusz Rybka ◽  
Ewa Kłodzińska ◽  
Dorota Sulejczak ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Healing Process ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

scholarly journals Immunohistological changes in skin wounds during the early periods of healing in a rat model

2012 ◽  
Vol 57 (No. 2) ◽  
pp. 77-82 ◽  
Author(s):  
F. Sabol ◽  
L. Dancakova ◽  
P. Gal ◽  
T. Vasilenko ◽  
M. Novotny ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wide Spectrum ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Skin Wound ◽  
Hair Follicles ◽  
In Vivo Model ◽  
Wound Healing Process ◽  
Rat Skin

The complexity of the wound healing process, which is still poorly understood, prompted us to perform an immunohistochemical investigation using rat skin as an in vivo model. Fifteen Sprague-Dawley rats were included in the experiment. Two round full thickness wounds, 4 mm in diameter, were made on the backs of all rats. Haematoxylin and eosin basic staining as well as antibodies against wide spectrum keratin, keratin 10, keratin&nbsp;14, &alpha;-smooth muscle actin, vimentin, fibronectin, collagens Type 1 and 3, and the transcription factor Sox-2 were applied to paraffin and frozen sections of skin wound specimens two, six and fourteen days after surgery, respectively. New hair follicles with Sox-2-positive cells were present after fourteen days; keratin/vimentin positivity was restricted to specimens of day two. Collagen-3 expression prevailed over collagen-1 expression at all evaluated time intervals, except in the uninjured part of the dermis. In conclusion, rat skin wound healing is a dynamic process which can serve as a model for studying phenomena such as cell-cell interactions and transitions in vivo.

scholarly journals Bionanomaterials: Advancements in Wound Healing and Tissue Regeneration

2021 ◽  
Author(s):  
Priyanka Chhabra ◽  
Kajol Bhati
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Wound Care ◽  
Health Care Organization ◽  
Healing Process ◽  
Volume Ratio ◽  
Wound Healing Process ◽  
Care Organization ◽  
Complex Process ◽  
High Degree

Abnormal wound healing represents a major healthcare issue owing to upsurge number of trauma and morbid physiology which ultimately posed a healthcare burden on patient, society and health care organization. A wound healing is a complex process so effective management of chronic wounds is often hard. Recently in addition to many conventional wound treatment’s advances in bionanomaterial are attaining much attention in wound care and skin tissue engineering. Bionanomaterials are biomolecule-based nanocomposite synthesized by plants, microbes and animals which possess high degree of biocompatibility, biodegradability, non-toxicity and bioactive assets. Bioactive assets like antimicrobial, immune modulatory, cell proliferation and angiogenesis of biomolecules forms fortunate microenvironment for the wound healing process. Nature has provided us with a significant set of biomolecules like chitosan, hyaluronic acid, collagen, cellulose, silk fucoidan etc. have been exploited to construct engineered bionanomaterials. These biopolymeric nanomaterials are currently researched comprehensively as they have higher surface to volume ratio and high chemical affinity showing a promising augmentation of deadly wounds. In this chapter we aimed to highlight the biological sources and bioengineering approaches adapted for biopolymers so they facilitate wound healing process.

Plasmin/plasminogen is essential for the healing of tympanic membrane perforations

2006 ◽  
Vol 96 (10) ◽  
pp. 512-519 ◽  
Author(s):  
Jinan Li ◽  
Per-Olof Eriksson ◽  
Annika Hansson ◽  
Sten Hellström ◽  
Tor Ny
Keyword(s):  
Wound Healing ◽  
Tympanic Membrane ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Keratinocyte Migration ◽  
Tympanic Membrane Perforations ◽  
Wounded Area ◽  
Deficient Mice

SummaryPlasminogen has been proposed to play an important role in different tissue remodeling processes such as wound healing and tissue regeneration after injuries. The healing of tympanic membrane perforations is a well-organized chain of inflammatory events, with an initial invasion of inflammatory cells followed by reparative and restoration phases. Here we show that the healing of tympanic membrane perforations is completely arrested in plasminogen-deficient mice, with no signs of any healing even 143 days after perforation. Inflammatory cells were recruited to the wounded area, but there were no signs of tissue debridement. In addition, removal of fibrin, keratinocyte migration and in-growth of connective tissue were impaired. This contrasts with skin wound healing, where studies have shown that, although the healing process is delayed, it reaches completion in all plasminogen-deficient mice. Our finding that keratinocyte migration and re-epithelialization were completely arrested in plasminogen-deficient mice indicates that plasminogen/plasmin plays a more profound role in the healing of tympanic membrane perforations than in the healing of other epithelial wounds.

Icariin-Loaded Polyvinyl Alcohol/Agar Hydrogel: Development, Characterization, and In Vivo Evaluation in a Full-Thickness Burn Model

2019 ◽  
Vol 18 (3) ◽  
pp. 323-335 ◽  
Author(s):  
Varuna Naga Venkata Arjun Uppuluri ◽  
T. S. Shanmugarajan
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Polyvinyl Alcohol ◽  
Tissue Regeneration ◽  
Healing Process ◽  
Wound Healing Process ◽  
Full Thickness ◽  
Burn Wound ◽  
In Vivo Evaluation ◽  
Hydrogel Scaffolds

Tissue regeneration has become a promising strategy for repairing damaged skin tissues. Among the hydrogels for tissue regeneration applications, topical hydrogels have demonstrated great potential for use as 3D-scaffolds in the burn wound healing process. Currently, no report has been published specifically on icariin-loaded polyvinyl alcohol (PVA)/agar hydrogel on full-thickness burn wounds. In the present study, burn tissue regeneration based on biomimetic hydrogel scaffolds was used for repairing damaged extracellular matrix. Furthermore, a skin burn model was developed in rats, and the icariin-loaded PVA/agar hydrogels were implanted into the damaged portions. The regeneration of the damaged tissues with the help of the icariin-loaded hydrogel group exhibited new translucent skin tissues and repaired extracellular matrix, indicating that the hydrogel can enhance the wound healing process. Moreover, characterization studies such as X-ray diffraction, Fourier-transformed infrared spectroscopy, and differential scanning calorimetry reported the extent of compatibility between icariin and its polymers. Results of the field emission scanning electron microscopy images revealed the extent of the spread of icariin within the polymer-based hydrogel. Furthermore, the wound healing potential, confirmed by histopathological and histochemical findings at the end of 21 days, revealed the visual evidence for the biomimetic property of icariin-loaded PVA/agar hydrogel scaffolds with the extracellular matrix for tissue regeneration.

A Study to Evaluate the Effectiveness of Sitz Bath on Episiotomy Wound Healing among Postnatal Mothers in Aravindan Hospital at Coimbatore

2021 ◽  
pp. 281-286
Author(s):  
Kalaivani L.
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sitz Bath ◽  
Highly Effective ◽  
Postnatal Mothers ◽  
Level Of Significance ◽  
And Control ◽  
Experimental Group

Statement of the problem: ‘A study to evaluate the effectiveness of sitz bath on episiotomy wound healing among postnatal mothers in Aravindan hospital at Coimbatore. Objectives of the study: 1. To assess the episiotomy wound healing among postnatal mothers both in experimental and control group. 2. To administer Sitz Bath Application to mothers in Experimental Group. 3. To evaluate the effectiveness of sitz bath on episiotomy wound healing among postnatal mothers in experimental group. 4. To compare the effectiveness of sitz bath on episiotomy wound healing among postnatal mothers between experimental and control group. 5. To find out the association between the post observational episiotomy wound healing scores of postnatal with selected demographic variable. Methodology: The quantitative approach - quasi-experimental, non- equivalent control group pre-test. Post-test design was selected to evaluate the effectiveness of sitzbath on Episiotomy wound healing among postnatal mothers The researcher adopted non probability convenient sampling technique and 60 postnatal mothers were selected for the study from Aravindan hospital Coimbatore. They are divided into two groups, 30 in experimental group and 30 in control group. The investigator used Reeda Scale and visual Analog pain scale to assess the episiotomy wound healing. Results: The findings reveal that application of sitz bath is highly effective on severe redness (63.3%), severe oedema (876%), severe ecchymosis (83.3%), severe discharge (93.3%) and poor approximation of wound (90%). Posttest on 5th day revealed that they had moderate redness (30%), moderate oedema (34.3%), mild ecchymosis (27.6%), moderate discharge (38.6%) and improved approximation of wound (27.6%). For all parameters of REEDA calculated t-value >p-value, which infers that application of sitz bath is statistically highly effective for healing of episiotomy at 0.05 level of significance. The calculated t-value is 22 > 2.05, which infers that application of sitz bath is statistically highly effective for healing of episiotomy wound at 0.05 level of significance. The application of sitz bath is highly effective in experimental group based on REEDA parameters as per redness (45%) in experimental group when compared to control group redness (90%), discharge (38.6) in experimental group when compared to control group discharge (53.3%), edema (34%) in experimental group when compared to control group odema. 6%), ecchymoseis 7.6%) in experimental group when compared to control group ecchymosis (53.3%) and on approximation of wound (27.6%) when compared to control group approximation of wound(47.6%). Hence H2 is accepted. Conclusion: This information shows that Application of Sitz Bath is effective in episiotomy wound healing process

scholarly journals The Role of Chemokines in Wound Healing

2018 ◽  
Vol 19 (10) ◽  
pp. 3217 ◽  
Author(s):  
Anisyah Ridiandries ◽  
Joanne Tan ◽  
Christina Bursill
Keyword(s):  
Wound Healing ◽  
Therapeutic Potential ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Wound Healing Process ◽  
Preclinical Research ◽  
Cxcr4 Antagonist ◽  
Impaired Wound Healing ◽  
Novel Approach ◽  
Healing Wounds

Wound healing is a multistep process with four overlapping but distinct stages: hemostasis, inflammation, proliferation, and remodeling. An alteration at any stage may lead to the development of chronic non-healing wounds or excessive scar formation. Impaired wound healing presents a significant health and economic burden to millions of individuals worldwide, with diabetes mellitus and aging being major risk factors. Ongoing understanding of the mechanisms that underly wound healing is required for the development of new and improved therapies that increase repair. Chemokines are key regulators of the wound healing process. They are involved in the promotion and inhibition of angiogenesis and the recruitment of inflammatory cells, which release growth factors and cytokines to facilitate the wound healing process. Preclinical research studies in mice show that the administration of CCL2, CCL21, CXCL12, and a CXCR4 antagonist as well as broad-spectrum inhibition of the CC-chemokine class improve the wound healing process. The focus of this review is to highlight the contributions of chemokines during each stage of wound healing and to discuss the related molecular pathologies in complex and chronic non-healing wounds. We explore the therapeutic potential of targeting chemokines as a novel approach to overcome the debilitating effects of impaired wound healing.

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