Use of Arbitrary Reference Genes May Lead to Misleading Conclusions

2019 ◽  
Vol 84 (5) ◽  
pp. 519-520 ◽  
Author(s):  
Shing Cheng Tan
Keyword(s):  
Reference Genes ◽  
Arbitrary Reference

Validation of Reference Genes in Chordoma

2014 ◽  
Vol 75 (S 02) ◽  
Author(s):  
Remco Santegoeds ◽  
Y. Yakkioui ◽  
J. Van Overbeeke ◽  
Y. Temel
Keyword(s):  
Reference Genes

Selection and validation of reference genes for accurate RT-qPCR gene expression normalization in cacao beans during fermentation

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Jocelyn De Wever ◽  
Tibo De Coninck ◽  
Helena Everaert ◽  
Emmanuel Ohene Afoakwa ◽  
Frauke Coppieters ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes

scholarly journals Author Correction: Selection and validation of reference genes by RT-qPCR for murine cementoblasts in mechanical loading experiments simulating orthodontic forces in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Christian Niederau ◽  
Rogerio B. Craveiro ◽  
Irma Azraq ◽  
Julia Brockhaus ◽  
Asisa Bastian ◽  
...  
Keyword(s):  
Mechanical Loading ◽  
Reference Genes ◽  
Orthodontic Forces

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Spatio-temporal selection of reference genes in the two congeneric species of Glycyrrhiza

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuping Li ◽  
Xiaoju Liang ◽  
Xuguo Zhou ◽  
Yu An ◽  
Ming Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Developmental Stage ◽  
Reference Genes ◽  
Developmental Stages ◽  
Individual Factors ◽  
Congeneric Species ◽  
Spatio Temporal ◽  
The Stability ◽  
Different Tissues ◽  
Selection Of

AbstractGlycyrrhiza, a genus of perennial medicinal herbs, has been traditionally used to treat human diseases, including respiratory disorders. Functional analysis of genes involved in the synthesis, accumulation, and degradation of bioactive compounds in these medicinal plants requires accurate measurement of their expression profiles. Reverse transcription quantitative real-time PCR (RT-qPCR) is a primary tool, which requires stably expressed reference genes to serve as the internal references to normalize the target gene expression. In this study, the stability of 14 candidate reference genes from the two congeneric species G. uralensis and G. inflata, including ACT, CAC, CYP, DNAJ, DREB, EF1, RAN, TIF1, TUB, UBC2, ABCC2, COPS3, CS, R3HDM2, were evaluated across different tissues and throughout various developmental stages. More importantly, we investigated the impact of interactions between tissue and developmental stage on the performance of candidate reference genes. Four algorithms, including geNorm, NormFinder, BestKeeper, and Delta Ct, were used to analyze the expression stability and RefFinder, a comprehensive software, provided the final recommendation. Based on previous research and our preliminary data, we hypothesized that internal references for spatio-temporal gene expression are different from the reference genes suited for individual factors. In G. uralensis, the top three most stable reference genes across different tissues were R3HDM2, CAC and TUB, while CAC, CYP and ABCC2 were most suited for different developmental stages. CAC is the only candidate recommended for both biotic factors, which is reflected in the stability ranking for the spatio (tissue)-temporal (developmental stage) interactions (CAC, R3HDM2 and DNAJ). Similarly, in G. inflata, COPS3, R3HDM2 and DREB were selected for tissues, while RAN, COPS3 and CS were recommended for developmental stages. For the tissue-developmental stage interactions, COPS3, DREB and ABCC2 were the most suited reference genes. In both species, only one of the top three candidates was shared between the individual factors and their interactions, specifically, CAC in G. uralensis and COPS3 in G. inflata, which supports our overarching hypothesis. In summary, spatio-temporal selection of reference genes not only lays the foundation for functional genomics research in Glycyrrhiza, but also facilitates these traditional medicinal herbs to reach/maximize their pharmaceutical potential.

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