A Practical Guide to the Role of Ancillary Techniques in the Diagnosis of Infectious Agents in Fine Needle Aspiration Samples

2019 ◽  
Vol 64 (1-2) ◽  
pp. 81-91
Author(s):  
Laurel J. Glaser ◽  
Kathleen T. Montone
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Molecular Techniques ◽  
Infectious Agents ◽  
Diagnostic Modality ◽  
Fine Needle ◽  
Additional Testing ◽  
Mass Lesions

Fine needle aspiration samples and small biopsies provide a minimally invasive diagnostic modality for mass lesions. When an infectious process is suspected based on initial evaluation, ancillary techniques can assist in making a specific diagnosis. Here we review the cytopathology that should prompt additional testing and review the availability and interpretation of special stains, immunohistochemistry, and in situ hybridization. In addition, this review addresses when special cultures may be necessary and the use of newer molecular techniques for pathogen identification.

ROLE OF FINE NEEDLE ASPIRATION CYTOLOGY (FNAC) IN BONE TUMORS AND TUMOR LIKE LESIONS

2021 ◽  
pp. 69-70
Author(s):  
Ragini Kumari ◽  
Vikash M. Harinandan ◽  
Kunal Shankar ◽  
Ajit Kumar Chaudhary ◽  
Debarshi Jana
Keyword(s):  
Fine Needle Aspiration ◽  
Bone Tumors ◽  
Needle Aspiration ◽  
Bone Lesions ◽  
Diagnostic Modality ◽  
Predictive Values ◽  
Female Ratio ◽  
Fine Needle ◽  
Needle Aspiration Cytology

Background: FNAC is considered an important procedure in the diagnosis of bone tumors because of its high accuracy. In our study FNA was performed in patients to study the role of FNAC in the diagnosis of bone tumors and to evaluate its usefulness as a diagnostic modality. Methods: Fine needle aspiration was performed on 54 patients whose age ranged between 5 – 75 years with a male to female ratio 1.84 :1 presented with various bone lesions. The bone lesions included 42 (78%) primary lesions, 5 (9.3%) metastatic deposits and 7 (12. 7%) malignancy of related structures involving the bone. Results: 47 biopsy specimens were available for cytohistological correlation and cytological ndings of 45 cases correlated with histopathology. The overall diagnostic accuracy was 95.92%, with 100% sensitivity and specicity. The predictive values of positive as well as negative test were 100%. Conclusions:Thus FNAwas found to be a safe and an extremely useful, rapid method in the preliminary diagnosis of bone lesions.

scholarly journals Role of endoscopic ultrasound-guided fine needle aspiration in the diagnosis of mass lesions

2016 ◽  
Vol 12 (2) ◽  
pp. 1085-1092 ◽  
Author(s):  
Chaoqun Han ◽  
Rong Lin ◽  
Qin Zhang ◽  
Jun Liu ◽  
Zhen Ding ◽  
...  
Keyword(s):  
Endoscopic Ultrasound ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrasound Guided ◽  
Fine Needle ◽  
Mass Lesions

scholarly journals Endoscopic/Endobronchial Ultrasound-Guided Fine Needle Aspiration and Ancillary Techniques, Particularly Flow Cytometry, in Diagnosing Deep-Seated Lymphomas

2016 ◽  
Vol 60 (4) ◽  
pp. 326-335 ◽  
Author(s):  
Ming Jin ◽  
Paul E. Wakely Jr.
Keyword(s):  
Flow Cytometry ◽  
Fine Needle Aspiration ◽  
Diagnostic Yield ◽  
Needle Aspiration ◽  
Molecular Techniques ◽  
Endobronchial Ultrasound ◽  
Fine Needle ◽  
T Cell Lymphomas ◽  
Reasonable Proportion

Evaluation of deep-seated lymphomas by fine-needle aspiration (FNA) can be challenging due to their reduced accessibility. Controversy remains as to whether FNA and ancillary techniques can be used to diagnose deep-seated lymphomas reliably and sufficiently for clinical management. Most published studies are favorable that endobronchial ultrasound (EBUS)/endoscopic ultrasound (EUS)-FNA plays an important role in the diagnosis of deep-seated lymphomas. The addition of ancillary techniques, particularly flow cytometry, increases diagnostic yield. While subclassification is possible in a reasonable proportion of cases, the reported rates of successful subclassification are lower than those for lymphoma detection/diagnosis. The diagnostic limitation exists for Hodgkin's lymphoma, grading of follicular lymphoma, and some T-cell lymphomas. The role of FNA in deep-seated lymphomas is much better established for recurrent than primary disease. It remains unclear whether the use of large-sized-needle FNA or a combination of core needle biopsy and FNA improves subclassification. It is important for cytopathologists to have considerable understanding of the WHO lymphoma classification and develop a collaborative working relationship with hematopathologists and oncologists. As EUS/EBUS-FNA techniques advance and sophisticated molecular techniques such as next- generation sequencing become possible, the role of FNA in the diagnosis of deep-seated lymphomas will possibly increase.

scholarly journals Role of fine needle aspiration cytology in bone lesions

2016 ◽  
Vol 3 (1) ◽  
pp. 26
Author(s):  
Veenu Jain ◽  
Tarun Agarwal
Keyword(s):  
Fine Needle Aspiration ◽  
Bone Tumors ◽  
Needle Aspiration ◽  
Bone Lesions ◽  
Diagnostic Modality ◽  
Predictive Values ◽  
Female Ratio ◽  
Fine Needle ◽  
Needle Aspiration Cytology

<p class="abstract"><strong>Background: </strong>FNAC is considered an important procedure in the diagnosis of bone tumors because of its high accuracy. In our study FNA was performed in patients to study the role of FNAC in the diagnosis of bone tumors and to evaluate its usefulness as a diagnostic modality<span lang="EN-IN">.</span></p><p class="abstract"><strong>Methods: </strong>Fine needle aspiration was performed on 54 patients whose age ranged between 5 – 75 years with a male to female ratio 1.84 :1 presented with various bone lesions. The bone lesions included 42 (78%) primary lesions, 5 (9.3%) metastatic deposits and 7 (12. 7%) malignancy of related structures involving the bone.<strong></strong></p><p class="abstract"><strong>Results: </strong>47 biopsy specimens were available for cytohistological correlation and cytological findings of 45 cases correlated with histopathology. The overall diagnostic accuracy was 95.92%, with 100% sensitivity and specificity. The predictive values of positive as well as negative test were 100%<span lang="EN-IN">.</span></p><p class="abstract"><strong>Conclusions:</strong> Thus FNA was found to be a safe and an extremely useful, rapid method in the preliminary diagnosis of bone lesions<span lang="EN-IN">.</span></p>

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