Identification of A Ring-Hydroxylating Dioxygenases Capable of Anthracene and Benz[a]anthracene Oxidization from Rhodococcus sp. P14

2018 ◽  
Vol 28 (4) ◽  
pp. 183-189 ◽  
Author(s):  
Tao Peng ◽  
An Luo ◽  
Jie Kan ◽  
Lei Liang ◽  
Tongwang Huang ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Carbon Source ◽  
Recombinant Protein ◽  
Aromatic Hydrocarbons ◽  
E Coli ◽  
Pah Degradation ◽  
The World ◽  
Polycyclic Aromatic ◽  
Single Carbon Source ◽  
Degradation Ability

Nowadays, contamination of soil and marine sediments by polycyclic aromatic hydrocarbons (PAHs) has become a serious problem all over the world. <i>Rhodococcus</i> sp. P14 was isolated from sediments with crude oil contaminate and showed degradation ability on various PAHs. The genome of <i>Rhodococcus</i> sp. P14 was sequenced. A gene cluster encoding a ring-hydroxylating dioxygenase Baa related to PAH degradation was identified by bioinformatics. The expression level of gene <i>baaA</i> was increased when P14 was cultured with anthracene, pyrene, phenanthrene, or benz[a]­anthracene as the single carbon source. The recombinant protein Baa was overexpressed in <i>E. coli</i> BL21 (DE3). Further investigations on the recombinant protein Baa in <i>E. coli</i> demonstrated that it was able to oxidize anthracene and benz [a]anthracene, resulting in 9,10-dihydroxyanthracene and 7, 12-dihydroxybenz[a]anthracene as metabolites, respectively. These results indicate that Baa plays an important role in PAH degradation in <i>Rhodococcus</i> sp. P14 and Baa has potential application in the bioremediation of PAHs in the contaminated environment.

scholarly journals POTENSI TIGA ISOLAT BAKTERI INDIGEN DARI KABUPATEN SIAK PROVINSI RIAU DALAM MENDEGRADASI NAFTALENA

Jurnal Kimia ◽  
2020 ◽  
pp. 94
Author(s):  
R. Novianty ◽  
B. Antika ◽  
. Saryono ◽  
A. Awaluddin ◽  
N. W. Pratiwi
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Optical Density ◽  
Aromatic Hydrocarbons ◽  
Growth Response ◽  
Final Concentration ◽  
Pseudomonas Sp ◽  
Joint Operation ◽  
Polycyclic Aromatic ◽  
Degradation Ability

Naphthalene is a group of Polycyclic Aromatic Hydrocarbons (PAHs) which is carcinogenic when presents in the environment. The purpose of this study was to determine the growth response and test the effectiveness of three isolates of indigen bacteria from the Joint Operation Agency area of ??PT. Siak Bumi Pusako-Pertamina Hulu (BOB BSP-PHE) in degrading naphthalene. The three isolates were tested in a Minimum Media (MM) liquid containing naphthalene with a final concentration of 0.2 mM and was incubated for 7 days. Optical Density (OD) and the degradation ability were analyzed using a UV-Vis spectrophotometer. The results showed that Pseudomonas sp. LBKURCC149 was able to grow in media containing naphthalene. Naphthalene is used as the only source of carbon and is able to degrade naphthalene by 21.98%.   Keywords: biodegradation, indigenous, naphthalene, Pseudomonas sp.

Impact of Inoculation Protocols, Salinity, and pH on the Degradation of Polycyclic Aromatic Hydrocarbons (PAHs) and Survival of PAH-Degrading Bacteria Introduced into Soil

1998 ◽  
Vol 64 (1) ◽  
pp. 359-362 ◽  
Author(s):  
Matthias Kästner ◽  
Maren Breuer-Jammali ◽  
Bernd Mahro
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Pore Water ◽  
Aromatic Hydrocarbons ◽  
Soil Bacteria ◽  
Water Salinity ◽  
Sphingomonas Paucimobilis ◽  
Pah Degradation ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic ◽  
Pore Water Salinity

ABSTRACT Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of the introduced bacteria were monitored in native and sterilized soil at different pHs. Introduction with mineral medium inhibited PAH degradation by the autochthonous microflora and by the strains tested. After introduction with water (without increase of the pore water salinity), no inhibition of the autochthonous microflora was observed and both strains exhibited PAH degradation.

scholarly journals Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils

2003 ◽  
Vol 69 (1) ◽  
pp. 275-284 ◽  
Author(s):  
Mikael Eriksson ◽  
Erik Sodersten ◽  
Zhongtang Yu ◽  
Gunnel Dalhammar ◽  
William W. Mohn
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Low Temperature ◽  
Aromatic Hydrocarbons ◽  
Intergenic Spacer ◽  
Aerobic Conditions ◽  
Enrichment Cultures ◽  
Pah Degradation ◽  
Reducing Conditions ◽  
Arctic Soil ◽  
Polycyclic Aromatic

ABSTRACT Thepotential for biodegradation of polycyclic aromatic hydrocarbons (PAHs)at low temperature and under anaerobic conditions is not wellunderstood, but such biodegradation would be very useful forremediation of polluted sites. Biodegradation of a mixture of 11different PAHs with two to five aromatic rings, each at a concentrationof 10 μg/ml, was studied in enrichment cultures inoculated withsamples of four northern soils. Under aerobic conditions, lowtemperature severely limited PAH biodegradation. After 90 days, aerobiccultures at 20°C removed 52 to 88% of the PAHs. The mostextensive PAH degradation under aerobic conditions at 7°C,53% removal, occurred in a culture from creosote-contaminatedsoil. Low temperature did not substantially limit PAH biodegradationunder nitrate-reducing conditions. Under nitrate-reducing conditions,naphthalene, 2-methylnaphthalene, fluorene, and phenanthrene weredegraded. The most extensive PAH degradation under nitrate-reducingconditions at 7°C, 39% removal, occurred in a culturefrom fuel-contaminated Arctic soil. In separate transfer cultures fromthe above Arctic soil, incubated anaerobically at 7°C, removalof 2-methylnaphthalene and fluorene was stoichiometrically coupled tonitrate removal. Ribosomal intergenic spacer analysis suggested thatenrichment resulted in a few predominant bacterial populations,including members of the genera Acidovorax,Bordetella, Pseudomonas, Sphingomonas, andVariovorax. Predominant populations from different soils oftenincluded phylotypes with nearly identical partial 16S rRNA genesequences (i.e., same genus) but never included phylotypes withidentical ribosomal intergenic spacers (i.e., different species orsubspecies). The composition of the enriched communities appeared to bemore affected by presence of oxygen, than by temperature or source oftheinoculum.

Cross hybridization of plasmid and genomic DNA from aromatic and polycyclic aromatic hydrocarbon degrading bacteria

1991 ◽  
Vol 37 (12) ◽  
pp. 924-932 ◽  
Author(s):  
J. M. Foght ◽  
D. W. S. Westlake
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Genomic Dna ◽  
Bacterial Strains ◽  
Cross Hybridization ◽  
Pah Degradation ◽  
Naphthalene Degradation ◽  
Degrading Bacteria ◽  
Wide Range ◽  
Polycyclic Aromatic

Forty-three bacterial strains were collected from various environmental and commercial sources and their ability to degrade polycyclic aromatic hydrocarbons (PAHs) was confirmed using the criteria of growth, mineralization, and oxidation. Undigested genomic DNA from these strains was blotted by Southern transfer to replicate membranes, which were probed either with purified plasmids (e.g., TOL and NAH7, associated with toluene and naphthalene degradation, respectively) or with genomic DNA from the other strains. The isolates were grouped according to hybridization and PAH-degradation results. One group of eight strains grew on naphthalene vapors as sole carbon source and hybridized with archetypical NAH plasmids. Another group of six isolates mineralized phenanthrene but could not grow on naphthalene, and their cryptic plasmids hybridized with Pseudomonas sp. HL7b, which degrades a wide range of PAHs. The remaining isolates, which could not grow on naphthalene but mineralized and (or) oxidized a variety of PAHs, hybridized with neither the pure plasmids nor heterologous genomic DNA, implying that their PAH-degradative genes were significantly dissimilar. This suggests that using TOL or NAH plasmids to probe an environmental population might reveal toluene- or naphthalene-degradative genes but would underestimate the occurrence of PAH-degradative genes. We suggest that a suite of probes would be necessary to evaluate the PAH-degradation potential of a mixed population. Key words: polycyclic aromatic hydrocarbons, degradative plasmids, NAH plasmid, TOL plasmid, hybridization.

KEMAMPUAN DAN KEKUATAN BIOREMEDIASI AGEN HAYATI JAMUR FUNGI PELAPUK PUTIH

2018 ◽  
Vol 4 (3) ◽  
Author(s):  
Donawati Dkk
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Environmental Pollutants ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Huge Number ◽  
Tropical Country ◽  
The World ◽  
Polycyclic Aromatic

Indonesia is a tropical country and the richest country in the world for biodiversity, including fl ora and fauna, invertebrates, phytoplankton, bacteria, and huge number of fungus such as mushrooms, molds, mildews and smuts. White rot fungi is one of the ubiquitous creatures in Indonesia. Results indicated that white rot fungus has ability to degrade successfully some organic pollutansin the environment, such as polycyclic aromatic hydrocarbons (PAH), Polychlorinated Biphenyls (PCB’s), Trinitrotoluence (TNT), and DDT. This organism has ability to release intra and extra-cellular enzymes. There are three important rules in these degradation processes, called lignin modifi ed enzymes (LME’s) including Lignin peroxidase, Mn-dependent peroxidase and Laccase. White rot fungi contains all three enzymes and able to breakdown and mineralize several environmental pollutants into non toxic forms. This paper discuss several processes responsible for this degradation.Key words: White rot fungi, bioremediation, lignin modifi ed enzymes, persistent organic pollutants.

scholarly journals Characterization of the Phenanthrene-Degrading Sphingobium yanoikuyae SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway

2020 ◽  
Vol 8 (6) ◽  
pp. 946 ◽  
Author(s):  
Chong Yin ◽  
Weiliang Xiong ◽  
Hua Qiu ◽  
Wanli Peng ◽  
Zixin Deng ◽  
...  
Keyword(s):  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Genome Mining ◽  
Whole Genome Sequence ◽  
Phenanthrene Degradation ◽  
Pah Degradation ◽  
Polycyclic Aromatic ◽  
Degradation Ability ◽  
Halogenated Aromatic Compounds

Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, Sphingobium yanoikuyae SJTF8 was isolated and identified with great and stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, and anthracene) and heterocyclic and halogenated aromatic compounds (dibenzothiophene and 9-bromophenanthrene) as the sole carbon source. It could degrade over 98% of 500 mg/L phenanthrene in 4 days, and the cis-3,4-dihydrophenanthrene-3,4-diol was the first-step intermediate. Notably, strain SJTF8 showed great tolerance to heavy metals and acidic pH. Supplements of 0.30 mM of Cu2+, 1.15 mM of Zn2+, and 0.01 mM of Cd2+ had little effect on its cell growth and phenanthrene degradation; phenanthrene of 250 mg/L could still be degraded completely in 48 h. Further, the whole genome sequence of S. yanoikuyae SJTF8 was obtained, and three plasmids were found. The potential genes participating in stress-tolerance and PAH-degradation were annotated and were found mostly distributed in plasmids 1 and 2. Elimination of plasmid 2 resulted in the loss of the PAH-degradation ability. On the basis of genome mining results, the possible degrading pathway and the metabolites of S. yanoikuyae SJTF8 to phenanthrene were predicted.

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