Effect of Physical and Chemical Hair Removal Methods on Skin Barrier Function in vitro: Consequences for a Hydrophilic Model Permeant

2018 ◽  
Vol 32 (1) ◽  
pp. 8-21 ◽  
Author(s):  
Astrid Pany ◽  
Victoria Klang ◽  
Marion Brunner ◽  
Johanna Ruthofer ◽  
Elisabeth Schwarz ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Hair Removal ◽  
Skin Barrier Function ◽  
Physical And Chemical

scholarly journals Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model

2021 ◽  
Vol 22 (23) ◽  
pp. 13091
Author(s):  
Andréa Tremblay ◽  
Mélissa Simard ◽  
Sophie Morin ◽  
Roxane Pouliot
Keyword(s):  
Docosahexaenoic Acid ◽  
Barrier Function ◽  
Culture Media ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Permeability ◽  
Skin Substitutes ◽  
Normal Human Skin ◽  
Assembly Method

Healthy skin moLEdels produced by tissue-engineering often present a suboptimal skin barrier function as compared with normal human skin. Moreover, skin substitutes reconstructed according to the self-assembly method were found to be deficient in polyunsaturated fatty acids (PUFAs). Therefore, in this study, we investigated the effects of a supplementation of the culture media with docosahexaenoic acid (DHA) on the barrier function of skin substitutes. To this end, 10 μM DHA-supplemented skin substitutes were produced (n = 3), analyzed, and compared with controls (substitutes without supplementation). A Franz cell diffusion system, followed by ultra-performance liquid chromatography, was used to perform a skin permeability to testosterone assay. We then used gas chromatography to quantify the PUFAs found in the epidermal phospholipid fraction of the skin substitutes, which showed successful DHA incorporation. The permeability to testosterone was decreased following DHA supplementation and the lipid profile was improved. Differences in the expression of the tight junction (TJ) proteins claudin-1, claudin-4, occludin, and TJ protein-1 were observed, principally a significant increase in claudin-1 expression, which was furthermore confirmed by Western blot analyses. In conclusion, these results confirm that the DHA supplementation of cell culture media modulates different aspects of skin barrier function in vitro and reflects the importance of n-3 PUFAs regarding the lipid metabolism in keratinocytes.

scholarly journals Transepidermal Water Loss Does Not Correlate with Skin Barrier Function In Vitro

2002 ◽  
Vol 118 (5) ◽  
pp. 871-875 ◽  
Author(s):  
Robert P. Chilcott ◽  
Christopher H. Dalton ◽  
Andrew J. Emmanuel ◽  
Ceri E. Allen ◽  
Simon T. Bradley
Keyword(s):  
Water Loss ◽  
Barrier Function ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Barrier Function

scholarly journals Partially Hydrolysed Whey-Based Infant Formula Improves Skin Barrier Function

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3113
Author(s):  
Sébastien Holvoet ◽  
Sophie Nutten ◽  
Lénaïck Dupuis ◽  
Dominique Donnicola ◽  
Tristan Bourdeau ◽  
...  
Keyword(s):  
Barrier Function ◽  
Protein Hydrolysate ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Barrier Function ◽  
Primary Keratinocytes ◽  
Total Ige ◽  
Expression Of Genes ◽  
Human Primary Keratinocytes

Specific partially hydrolysed whey-based infant formulas (pHF-W) have been shown to decrease the risk of atopic dermatitis (AD) in infants. Historically, AD has been associated primarily with milk allergy; however, defective skin barrier function can be a primary cause of AD. We aimed to ascertain whether oral supplementation with pHF-W can improve skin barrier function. The effect of pHF-W was assessed on transepidermal water loss (TEWL) and antibody productions in mice epicutaneously exposed to Aspergillus fumigatus. Human primary keratinocytes were stimulated in vitro, and the expression of genes related to skin barrier function was measured. Supplementation with pHF-W in neonatal mice led to a significant decrease in TEWL and total IgE, but not in allergen-specific antibody levels. The whey hydrolysate was sufficient to decrease both TEWL and total IgE. Aquaporin-3 gene expression, linked with skin hydration, was modulated in the skin of mice and human primary keratinocytes following protein hydrolysate exposure. Skin barrier improvement may be an additional mechanism by which pHF-W may potentially reduce the risk of AD development in infants. Further human studies are warranted to confirm the clinical efficacy of these observations.

The Role of Clindamycin Phosphate Associated with Adapalene in Three Semisolid Formulations Developed for Topical Acne Treatment

2017 ◽  
Vol 68 (5) ◽  
pp. 937-943
Author(s):  
Stela Mariana Al Hussein ◽  
Nicoleta Todoran ◽  
Silvia Imre ◽  
Hussam Al Hussein ◽  
Ana Melero Zaera ◽  
...  
Keyword(s):  
Barrier Function ◽  
Antimicrobial Agents ◽  
Acne Vulgaris ◽  
Skin Barrier ◽  
Human Epidermis ◽  
Skin Barrier Function ◽  
Clindamycin Phosphate ◽  
Oil In Water ◽  
Study Results

Despite the fact that in mild-to moderate acne vulgaris the standard first-line therapy is the topical treatment with fixed combinations of antimicrobial agents and retinoids, the skin type and the skin barrier function should be taken into account when formulating a topical product. The aim of this study was the comparison of three new semisolid formulations developed for topical application by evaluation of their rheological behavior, as well as the evaluation of in vitro percutaneous diffusion through human epidermis membrane of the pharmaceutical ingredients. Clindamycin phosphate and adapalene were incorporated in three different topical bases, an HPLC method for the determination of their content in the new formulations being developed and validated. A higher concentration of drugs was released from the two gel systems (hydroxypropylmethylcellulose 2.5% -F1 and hydroxyethylcellulose 3% -F2) than from the oil-in-water cream (F3) at pH 7.4, whereas at pH 5.5 the drugs were released in higher amounts from the formulation F3. Following the rheological behavoir associated with the penetrability through the human epidermis membrane, our study results suggest that F1 and F2 could be appropriate in treating acne lesions in patients with oily skin and unaffected skin barrier function. In contrast, the oil-in-water cream (F3), due to its possible emolient effect and its higher penetrability at pH 5.5 than gel vehicles, may be indicated for patients with dry and sensitive skin associated with an altered skin barrier.

A Novel Multi-Action Emollient Plus Cream Improves Skin Barrier Function in Patients with Atopic Dermatitis: In vitro and Clinical Evidence

2021 ◽  
Vol 34 (1) ◽  
pp. 8-18
Author(s):  
Marika Quadri ◽  
Roberta Lotti ◽  
Laura Bonzano ◽  
Silvana Ciardo ◽  
Mario Bruno Guanti ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Barrier Function ◽  
Randomized Study ◽  
Skin Barrier ◽  
Lower Limbs ◽  
Skin Barrier Function ◽  
Tape Stripping ◽  
Epidermal Thickness ◽  
Epidermal Homeostasis

Background: Emollients capable of restoring the skin barrier function would extend their role beyond basic maintenance therapy in atopic dermatitis (AD). Objectives: Investigate the effect of a novel emollient plus cream (EC; Dermoflan®) on the skin barrier in vitro and in patients with mild-to-moderate AD. Methods: The effect of EC on the skin barrier recovery was evaluated using a tape-stripping (TS) model. After TS, organ cultures were treated with EC (undiluted or diluted 1:1 with water) and analyzed at 18–120 h using hematoxylin and eosin, Oil Red O, immunohistochemical, and immunofluorescent techniques. In a double-blind, randomized study, EC or placebo was applied once daily for 2 months to antecubital folds of the upper and lower limbs of patients with mild-to-moderate AD in clinical remission. Epidermal thickness, vascularization, and epidermal hydration were assessed by optical coherence tomography and corneometry, respectively, at baseline, and 1 and 2 months following treatment initiation. Results: Following TS, EC treatment significantly increased epidermal thickness and lipid content versus diluent in the skin organ culture, as well as claudin-1, involucrin, and caspase-14 expression, suggesting skin barrier repair. EC treatment also decreased keratin-16 expression and increased levels of Toll-like receptors 1 and 2 versus diluent, suggesting involvement in regulating the epidermal immune response. In 20 patients randomized 1:1 to EC or placebo, EC treatment at the elbow fold/popliteal fossa significantly decreased epidermal thickness after 2 months, and the number of blood vessels at the elbow fold after 1 and 2 months, versus placebo. EC significantly improved the skin hydration after 2 months versus baseline. Conclusions: This novel multi-action EC may help to restore epidermal homeostasis and improve the skin of patients with AD. Results indicate that this novel multi-action EC could be a valid adjuvant therapy in patients with AD. Key Message: Novel multi-action emollient cream helps to restore epidermal homeostasis and improves the skin affected by AD.

scholarly journals Proteoglycan Combined with Hyaluronic Acid and Hydrolyzed Collagen Restores the Skin Barrier in Mild Atopic Dermatitis and Dry, Eczema-Prone Skin: A Pilot Study

2021 ◽  
Vol 22 (19) ◽  
pp. 10189
Author(s):  
Young In Lee ◽  
Sang Gyu Lee ◽  
Jemin Kim ◽  
Sooyeon Choi ◽  
Inhee Jung ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Hyaluronic Acid ◽  
Barrier Function ◽  
Ex Vivo ◽  
Therapeutic Option ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Barrier Function ◽  
Hydrolyzed Collagen

Dry and eczema-prone skin conditions such as atopic dermatitis and xerotic eczema primarily indicate an impaired skin barrier function, which leads to chronic pruritus. Here, we investigated the effects of a novel emollient containing H.ECMTM liposome, which contains a soluble proteoglycan in combination with hydrolyzed collagen and hyaluronic acid. A prospective, single-arm study was conducted on 25 participants with mild atopic dermatitis or dry skin to assess the hydration and anti-inflammatory effect of the novel emollient applied daily over four weeks. All efficacy parameters, including itching severity, transepidermal water loss, and skin hydration, improved significantly after four weeks. The in vitro and ex vivo studies confirmed the restoration of the skin’s barrier function. The study revealed the clinical and laboratory efficacy of H.ECMTM liposome in reducing itching and improving the skin’s barrier integrity. Thus, the use of H.ECMTM liposome can be considered a therapeutic option for dry and eczema-prone skin.

scholarly journals In Vitro Effects of Dehydrotrametenolic Acid on Skin Barrier Function

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4583 ◽  
Author(s):  
Eunju Choi ◽  
Young-Gyu Kang ◽  
So-Hyeon Hwang ◽  
Jin Kyeong Kim ◽  
Yong Deog Hong ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Barrier Function ◽  
Skin Barrier ◽  
Keratinocyte Differentiation ◽  
Luciferase Reporter ◽  
Skin Barrier Function ◽  
Hacat Cells ◽  
Differentiation Markers ◽  
Triterpene Acid

Dehydrotrametenolic acid (DTA) is a lanostane-type triterpene acid isolated from Poria cocos Wolf (Polyporaceae). Several studies have reported the anti-inflammatory and antidiabetic effects of DTA; however, its effects on the skin are poorly understood. In this study, we investigated the effects of DTA on skin barrier function in vitro and its regulatory mechanism in human keratinocyte cell line HaCaT cells. DTA increased the microRNA (mRNA) expression of natural moisturizing factor-related genes, such as HAS-2, HAS-3, and AQP3 in HaCaT cells. DTA also upregulated the mRNA expression of various keratinocyte differentiation markers, including TGM-1, involucrin, and caspase-14. Moreover, the protein expression of HAS-2, HAS-3, and TGM-2 were significantly increased by DTA. To examine the regulatory mechanisms of DTA, Western blotting, luciferase-reporter assays, and RT-PCR were conducted. The phosphorylation of mitogen-activated protein kinases (MAPKs) and IκBα were increased in DTA-treated HaCaT cells. In addition, AP-1 and NF-κB transcriptional factors were dose-dependently activated by DTA. Taken together, our in vitro mechanism studies indicate that the regulatory effects of DTA on skin hydration and keratinocyte differentiation are mediated by the MAPK/AP-1 and IκBα/NF-κB pathways. In addition, DTA could be a promising ingredient in cosmetics for moisturizing and increased skin barrier function.

scholarly journals Role of AHR Ligands in Skin Homeostasis and Cutaneous Inflammation

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3176
Author(s):  
Nieves Fernández-Gallego ◽  
Francisco Sánchez-Madrid ◽  
Danay Cibrian
Keyword(s):  
Atopic Dermatitis ◽  
Barrier Function ◽  
Inflammatory Responses ◽  
Normal Skin ◽  
Skin Barrier ◽  
Skin Inflammation ◽  
In Vitro Assays ◽  
Skin Barrier Function

Aryl hydrocarbon receptor (AHR) is an important regulator of skin barrier function. It also controls immune-mediated skin responses. The AHR modulates various physiological functions by acting as a sensor that mediates environment–cell interactions, particularly during immune and inflammatory responses. Diverse experimental systems have been used to assess the AHR’s role in skin inflammation, including in vitro assays of keratinocyte stimulation and murine models of psoriasis and atopic dermatitis. Similar approaches have addressed the role of AHR ligands, e.g., TCDD, FICZ, and microbiota-derived metabolites, in skin homeostasis and pathology. Tapinarof is a novel AHR-modulating agent that inhibits skin inflammation and enhances skin barrier function. The topical application of tapinarof is being evaluated in clinical trials to treat psoriasis and atopic dermatitis. In the present review, we summarize the effects of natural and synthetic AHR ligands in keratinocytes and inflammatory cells, and their relevance in normal skin homeostasis and cutaneous inflammatory diseases.

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