scholarly journals Coreopsis Tinctoria Modulates Lipid Metabolism by Decreasing Low-Density Lipoprotein and Improving Gut Microbiota

2018 ◽  
Vol 48 (3) ◽  
pp. 1060-1074 ◽  
Author(s):  
Zhigang Ren ◽  
Yali Li ◽  
Jiangyun Liu ◽  
Haitao Li ◽  
Ang Li ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Gut Microbiota ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Low Density ◽  
Biochemical Tests ◽  
Coreopsis Tinctoria ◽  
Normal Controls

Background/Aims: The prevalence of hyperlipidemia is increasing rapidly. The role of Coreopsis tinctoria (CT) in amending lipid metabolism in hyperlipidemia patients has not been reported. This study aims to evaluate the role of CT in altering lipid metabolism in hyperlipidemia patients and to explore the possible mechanisms mediated by gut microbiota in hyperlipidemia mice models. Methods: A retrospective analysis in 40 hyperlipidemia patients was conducted, in which 20 patients took fenofibrate and another 20 patients normatively drank water with CT. Hyperlipidemia mice models were also established. Blood biochemical tests were performed using an automatic biochemical analyzer. Liver histopathology was observed by hematoxylin and eosin staining. Ileocecal samples were collected from mice, and bacterial DNA was extracted and sequenced by MiSeq sequencing. Bacterial composition and differences were analyzed. Results: In hyperlipidemia patients, CT was associated with decreased triglyceride and low-density lipoprotein (LDL) levels without liver injury. The experimental hyperlipidemia model also verified a similar result. Gut microbial richness and diversity were significantly decreased in hyperlipidemic mice, but increased after CT treatment. Bacterial communities were significantly differentiated between normal controls and hyperlipidemic mice. CT administration improved gut microbiota composition to an approximately normal status. Meanwhile, CT administration attenuated bacterial alterations at the class, order, family, and genus levels in hyperlipidemic mice. Importantly, the genera Barnesiella, Lactobacillus, and Helicobacter achieved high discriminatory power in hyperlipidemic mice relative to normal controls. Conclusions: CT can modulate blood lipid metabolism with improvement of liver function by decreasing LDL and improving gut microbiota compositions. These findings may provide novel therapeutic strategies for patients with hyperlipidemia.

scholarly journals SAT0521 EVALUATION OF LIPID PROFILE IN PATIENTS WITH AVASCULAR NECROSIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1216.2-1217
Author(s):  
K. Gudkevich ◽  
N. Martusevich ◽  
E. Dashkevich
Keyword(s):  
Lipid Metabolism ◽  
Avascular Necrosis ◽  
Cardiovascular Events ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Joint Involvement ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Background:Avascular necrosis (AN) is a disabling disease affecting predominantly young men. It steadily progresses to secondary osteoarthritis. The rate of disability is high, because hip joint is most commonly affected by AN; moreover, the rate of bilateral hip joint involvement is also high. The key event in the pathogenesis of AN is circulation disorder which results in ischemia and, furthermore, death of bone tissue.Nowadays the data about lipid metabolism in patients with AN. Besides, there is contradictory data about the role of homocysteine in the risk of cardiovascular events. Based on these, it is topical to investigate lipid metabolism and endothelial function in patients with AN.Objectives:The aim of the present study was to investigate lipid metabolism and endothelial function in patients with different stages of AN in order to find risk factors for incidence and progression of this disease.Methods:We recruited 41 patients with AN of the femoral head or femoral condyles aged 51 (35-64) years, 56,1% (n=23) were male. The inclusion criteria was diagnosis of AN confirmed by X-Ray, CT or MRI. The exclusion criteria were: traumatic AN, Legg-Calve-Pertes disease, connective tissue systemic diseases. Also we divided patients into subgroups with early (39% (n=16)) and late (61,0% (n=25)) stages of AN.Patients of the control group (n=32) were comparable by age and gender. The data was proceeded using non-parametric and descriptive statistics. We evaluated the following parameters: homocysteine, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, cholesterol ratio, apolipoprotein A1, apolipoprotein B.Results:In patients with AN we observed higher level of homocysteine (12,119 (7,771-14,38) vs. 7,911 (6,612-10,54) mcmol/l, p=0,05). The results are consistent with the data that higher homocyseine level is associated with higher risk for cardiovascular events (1). There is literature data about elevated homocysteine level in patients with AN, which was associated with lower levels of vitamin B12 and folic acid. Thus, interpretation of homocystene significance is more reliable in association with vitamin B12 and folic acid levels.Also, we observed that level of HDLP-C is higher in patients with early stages than in pate stages of AN (1,55 (1,31-1,57) vs. 1,23 (1,04-1,36) mmol/l, р=0,04). Thus, HDLP-C can be interpreted as a protective factor against contralateral joint involvement in AN patients. The data obtained is consistent with the existing data that HDLP-C lowers the risk for cardiovascular events.Conclusion:The data obtained indicate a significant role of dyslypidemia in the pathogenesis of AN in the context of vascular theory.References:[1]Steven G. Chrysant & George S. Chrysant (2018) The current status of homocysteine as a risk factor for cardiovascular disease: a mini review, Expert Review of Cardiovascular Therapy, 16:8, 559-565, DOI: 10.1080/14779072.2018.1497974.[2]Narayanan, A., Khanchandani, P., Borkar, R.M.et al.Avascular Necrosis of Femoral Head: A Metabolomic, Biophysical, Biochemical, Electron Microscopic and Histopathological Characterization.Sci Rep7, 10721 (2017).https://doi.org/10.1038/.Disclosure of Interests:Katsiarina Gudkevich: None declared, Natalia Martusevich Shareholder of: k, Elena Dashkevich: None declared

Emodin alleviated hyperlipidemia and hepatic lipid metabolism in zebrafish larvae fed a high-cholesterol diet via AMPK/SREBP-2/PCSK9/LDLR signaling pathway

2021 ◽  
Author(s):  
Linfeng He ◽  
Cheng Wang ◽  
Yafang Zhang ◽  
Chaocheng Guo ◽  
Yan Wan ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Cholesterol Diet ◽  
Low Density ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Accumulation ◽  
Hepatic Lipid ◽  
Zebrafish Larvae

Abstract BackgroundEmodin (EM) is one of bioactive components extracted from Rheum palmatum L. (Dahuang), which possesses numerous pharmacological activities including hypolipidemic effect. However, the potential action of EM on hyperlipidemia (HLP) remains unclear. Here, the theraputic effect of EM against HLP were investigated.MethodsIn this study, the hypolipidemic properties of EM were evaluated using high-cholesterol diet (HCD)-stimulated zebrafish larvae model. The body weight, body length and body mass index (BMI) was measured. The total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) as well as the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by corresponding assay kits. Tg (flil: eGFP) zebrafish were utilized to observe vascular cholesterol accumulation and Tg (mpx: eGFP) zebrafish to visualize and quantify neutrophil inflammation. The hepatic lipid deposition and hepatic histopathology were analyzed by Oil red O staining and H&E staining, respectively. Finally, the underlying mechanism of EM were investigated using real-time quantitative PCR (RT-qPCR) analysis to assess the gene levels of adenosine monophosphate-activated protein kinase alpha (AMPKα), sterol regulatory element binding protein 2 (SREBP-2), proprotein convertase subtilisin kexin 9 (PCSK9), low-density lipoprotein receptor (LDLR), 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR), adenosine triphosphate binding cassette transporter A1 (ABCA1) and adenosine triphosphate binding cassette transporter G1 (ABCG1).ResultsOur data indicated that EM reduced obesity of zebrafish as evidenced by the decrease in body weight, body length and BMI. EM significantly reduced TC, TG, and LDL-C, and increased HDL-C contents. Moreover, it displayed a prominent inhibitory effect on blood cholesterol accumulation, hepatic lipid accumulation, and neutrophil inflammation in vascular site. Additionally, EM improved the liver function through decreasing ALT and AST levels of zebrafish with HCD-induced hepatosteatosis. Further investigation showed that EM treatment attenuated lipid accumulation via upregulating the expression of AMPKα, LDLR, ABCA1 and ABCG1, and downregulating the expression of SREBP-2, PCSK9 and HMGCR.ConclusionTo conclude, EM alleviated lipid metabolism disorder symptoms caused by HCD via modulating AMPK/SREBP-2/PCSK9/LDLR pathway in larvae, suggesting that EM may be developed into hypolipidmic agent for treating lipid metabolism related diseases.

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