Biomarker Discovery by Mass Spectrometry in Cerebrospinal Fluid and Plasma after Global Hypoxia-Ischemia in Newborn Piglets

Neonatology ◽  
2018 ◽  
Vol 114 (4) ◽  
pp. 307-314
Author(s):  
Kasper Jacobsen Kyng ◽  
Anders Valdemar Edhager ◽  
Tine Brink Henriksen ◽  
Christer Zøylner Swan ◽  
Niels Gregersen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Biomarker Discovery ◽  
Newborn Piglets ◽  
Hypoxia Ischemia

Application of an End-to-End Biomarker Discovery Platform to Identify Target Engagement Markers in Cerebrospinal Fluid by High Resolution Differential Mass Spectrometry

2010 ◽  
Vol 9 (3) ◽  
pp. 1392-1401 ◽  
Author(s):  
Cloud P. Paweletz ◽  
Matthew C. Wiener ◽  
Andrey Y. Bondarenko ◽  
Nathan A. Yates ◽  
Qinghua Song ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
High Resolution ◽  
Biomarker Discovery ◽  
Target Engagement ◽  
End To End

Chemical profiling of cerebrospinal fluid by multiple reaction monitoring mass spectrometry

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5252-5255 ◽  
Author(s):  
Christina R. Ferreira ◽  
Karen E. Yannell ◽  
Brit Mollenhauer ◽  
Ryan D. Espy ◽  
Fernanda B. Cordeiro ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Biomarker Discovery ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Chemical Profiling

We report an accelerated biomarker discovery workflow and results of sample screening by mass spectrometry based on multiple reaction monitoring (MRM).

scholarly journals Tandem mass spectrometry-based identification of protein profiles in the cerebrospinal fluid of Alzheimer’s dementia patients

2021 ◽  
Author(s):  
Anamika Misra ◽  
Sankha Shubhra Chakrabarti ◽  
Indrajeet Singh Gambhir ◽  
Meghraj Singh Baghel ◽  
Yugendra Ramchandra Patil
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Growth Factor ◽  
Tandem Mass Spectrometry ◽  
Biomarker Discovery ◽  
Early Stage ◽  
Enrichment Analysis ◽  
Tandem Mass ◽  
Insulin Like Growth Factor ◽  
Proteomic Approach

Abstract Background: Alzheimer’s disease (AD) is the most common form of dementia and about two thirds cases are diagnosed late due to a long asymptomatic phase. There exists the need for newer biomarkers which can add accuracy to AD diagnosis, detect AD at an early stage, as also lend new pathogenic insights into AD. Recent AD biomarker discovery has focused on proteomic approaches, especially in the cerebrospinal fluid. Methods: We used a bottom-up proteomic approach. Cerebrospinal fluid (CSF) samples from 6 patients with AD and 6 controls were digested with trypsin and analyzed by using LC-MS/MS (tandem mass spectrometry). The peptide data from CSF samples of both AD and control groups was then subjected to bioinformatics analysis with STRING version 11.0. Protein-protein interaction networks were constructed, and enrichment analysis performed.Results: Significant up-regulation of 13 proteins in the CSF was observed in AD cases in comparison to controls, while 30 proteins were down-regulated. APOE and LGALS3BP were the upregulated proteins involved in closed network and the downregulated proteins were F2, PENK, IGF2, APOH, SAA1, AHSG, SPP1 and CD44. APOE, APOH, F2 and PENK shared common involvement in multiple biological processes as evident on enrichment. Regulation of insulin like growth factor involving IGF2, F2, APOE and AHSG and glycosaminoglycan binding involving APOE, APOH, F2, SAA1, and CD44 were major pathways of interest determined on bioinformatic analysis.Conclusion: Our study identified novel tentative biomarkers of AD which included F2, PENK and SAA1, as well as reinforced earlier described biomarkers such as APOE and AHSG. These findings need to be validated in larger sample sizes to evaluate their utility as true biomarkers. Further, the pathways of interest- insulin like growth factor regulation and glycosaminoglycan binding need to be studied further in the context of AD.

Concentrations of non-glucose polyols in serum and cerebrospinal fluid from apparently healthy adults and children.

1984 ◽  
Vol 30 (2) ◽  
pp. 188-191 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
S Seki ◽  
K Seki
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
Metabolic Diseases ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Adults And Children ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Six non-glucose polyols--mannose, fructose, 1-deoxyglucose, mannitol, glucitol, and inositol--were identified and evaluated in human serum and cerebrospinal fluid by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Concentrations of fructose, mannose, and inositol in the serum of healthy persons or children without metabolic diseases varied with age, as already reported for 1-deoxyglucose. Fructose, inositol, and glucitol concentrations in cerebrospinal fluid significantly exceeded those in serum. The method described here for determining polyols and for evaluating polyol patterns in serum, as well as the resulting data on children and healthy subjects, should be useful in investigations of the clinical and physiological significance of polyols.

Sign in / Sign up

Export Citation Format

Share Document