scholarly journals Quantitative Large-Scale Three-Dimensional Imaging of Human Kidney Biopsies: A Bridge to Precision Medicine in Kidney Disease

Nephron ◽  
2018 ◽  
Vol 140 (2) ◽  
pp. 134-139 ◽  
Author(s):  
Seth Winfree ◽  
Pierre C. Dagher ◽  
Kenneth W. Dunn ◽  
Michael T. Eadon ◽  
Michael Ferkowicz ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Precision Medicine ◽  
Large Scale ◽  
Three Dimensional ◽  
Human Kidney ◽  
Three Dimensional Imaging

Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline

2021 ◽  
Author(s):  
Michael J. Ferkowicz ◽  
◽  
Seth Winfree ◽  
Angela R. Sabo ◽  
Malgorzata M. Kamocka ◽  
...  
Keyword(s):  
Large Scale ◽  
Three Dimensional ◽  
Human Kidney

Novel three-dimensional imaging volumetry in autosomal dominant polycystic kidney disease: comparison with 2D volumetry

2014 ◽  
Vol 82 (08) ◽  
pp. 98-106
Author(s):  
Dongsuk Shin ◽  
Kyu-Beck Lee ◽  
Young Youl Hyun ◽  
Young Rae Lee ◽  
Young-Hwan Hwang ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Polycystic Kidney Disease ◽  
Autosomal Dominant ◽  
Three Dimensional ◽  
Polycystic Kidney ◽  
Three Dimensional Imaging ◽  
Autosomal Dominant Polycystic Kidney

scholarly journals Three-dimensional imaging of subsurface structural patterns using quantitative large-scale multiconfiguration electromagnetic induction data

2014 ◽  
Vol 50 (3) ◽  
pp. 2732-2748 ◽  
Author(s):  
Christian von Hebel ◽  
Sebastian Rudolph ◽  
Achim Mester ◽  
Johan A. Huisman ◽  
Pramod Kumbhar ◽  
...  
Keyword(s):  
Electromagnetic Induction ◽  
Large Scale ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Structural Patterns

scholarly journals An atlas of healthy and injured cell states and niches in the human kidney

2021 ◽  
Author(s):  
Blue B. Lake ◽  
Rajasree Menon ◽  
Seth Winfree ◽  
Qiwen Hu ◽  
Ricardo Melo Ferreira ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Immune Responses ◽  
Large Scale ◽  
3D Imaging ◽  
Kidney Injury ◽  
Human Kidney ◽  
Cell Types ◽  
Molecular Signatures ◽  
Imaging Analysis ◽  
Future Studies

Understanding kidney disease relies upon defining the complexity of cell types and states, their associated molecular profiles, and interactions within tissue neighborhoods. We have applied multiple single-cell or -nucleus assays (>400,000 nuclei/cells) and spatial imaging technologies to a broad spectrum of healthy reference (n = 42) and disease (n = 42) kidneys. This has provided a high resolution cellular atlas of 100 cell types that include rare and novel cell populations. The multi-omic approach provides detailed transcriptomic profiles, epigenomic regulatory factors, and spatial localizations for major cell types spanning the entire kidney. We further identify and define cellular states altered in kidney injury, encompassing cycling, adaptive or maladaptive repair, transitioning and degenerative states affecting several segments. Molecular signatures of these states permitted their localization within injury neighborhoods using spatial transcriptomics, and large-scale 3D imaging analysis of ~1.2 million neighborhoods provided linkages to active immune responses. These analyses further defined biological pathways relevant to injury niches, including signatures underlying the transition from reference to predicted maladaptive states that were associated with a decline in kidney function during chronic kidney disease. This human kidney cell atlas, including injury cell states and neighborhoods, will be a valuable resource for future studies.

scholarly journals Three-dimensional morphometric analysis of human kidney nephron structures in health and disease

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Suneil Bhaskara ◽  
Michael Ferkowicz ◽  
Daria Barwinska ◽  
Tarek M. Ashkar (El-Achkar)
Keyword(s):  
Kidney Disease ◽  
3D Imaging ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Kidney Tissue ◽  
Human Kidney ◽  
The United States ◽  
Therapeutic Interventions ◽  
Tubular Atrophy ◽  
Tissue Clearing

Background and Hypothesis: Chronic kidney disease (CKD) is very common and affects as many as 37 million people in the United States. Diabetes and hypertension are the most common causes of CKD.  The pathogenesis of CKD is not fully elucidated. Morphological changes such as glomerulosclerosis and tubular atrophy are commonly observed with advanced disease. However, it is unclear if such changes occur at earlier stages of disease, a time when therapeutic interventions are likely to have the most benefit. Measurements of glomerular, vascular and tubular dimensions have been typically derived from thin section, but a pipeline for acquiring these morphometric measurements in 3-dimentions have not been performed. Using enhanced tissue clearing, confocal 3D imaging and volumetric segmentation and analysis, we aimed at developing an approach to measure the dimensions of various structures within the human kidney. Our overarching hypothesis is that such approach could allow the detection of morphometric changes in early disease. Experimental Design or Project Methods: Kidney tissue were used either from donor nephrectomies or stored frozen biopsies from Biobank Biopsy Cohort of Indiana (BBCI). Enhanced tissue clearing and staining for nuclei, endothelium and  proximal tubules were performed. Optical sectioning and 3D Imaging was done using confocal microscopy. Volume rendering, segmentation and analysis were done using the Imaris Software (Bitplane). Various built in and customized segmentation approaches were used. Results: Volumes spanning 200 µm in thickness encompassing entire glomeruli and tubules were obtained. 3D Rendering of these volumes allowed visualization and enabled 3D segmentation. Dimensions of entire human glomeruli (maximum and minimum diameters, glomerular volumes and cellular densities), tubules and vessels were defined in control and samples with diabetic kidney disease. Conclusion and Potential Impact: Our studies established an approach for accurate measurements of the dimensions of nephronal structures preserved in their 3D environment within tissue. We also established the feasibility of this approach in comparing changes with disease vs. control.  Such methodology will open up a new line of investigations to better understand the pathogenesis of CKD, particularly at its early stages.  

The Role of Three-Dimensional Imaging in Evaluation of the Sinonasal Mass

1996 ◽  
Vol 34 (1) ◽  
pp. 27
Author(s):  
Sue Yon Shim ◽  
Ki Joon Sung ◽  
Young Ju Kim ◽  
In Soo Hong ◽  
Myung Soon Kim ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Sinonasal Mass

Synchrotron and neutron laminography for three-dimensional imaging of devices and flat material specimens

2012 ◽  
Vol 103 (2) ◽  
pp. 170-173 ◽  
Author(s):  
Lukas Helfen ◽  
Thilo F. Morgeneyer ◽  
Feng Xu ◽  
Mark N. Mavrogordato ◽  
Ian Sinclair ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging
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