Modulation of Pacemaker Potentials in Murine Small Intestinal Interstitial Cells of Cajal by Gamisoyo-San, a Traditional Chinese Herbal Medicine

Digestion ◽  
2018 ◽  
Vol 98 (1) ◽  
pp. 56-68 ◽  
Author(s):  
Doeun Kim ◽  
Jung Nam Kim ◽  
Joo Hyun Nam ◽  
Jong Rok Lee ◽  
Sang Chan Kim ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Pacemaker Potentials ◽  
Small Intestinal ◽  
Cells Of Cajal

scholarly journals Effects of Ca2+-Activated Cl- Channel ANO1inhibitors on Pacemaker Activity in Interstitial Cells of Cajal

2018 ◽  
Vol 51 (6) ◽  
pp. 2887-2899 ◽  
Author(s):  
Seok Choi ◽  
Hyun Goo Kang ◽  
Mei Jin Wu ◽  
Han Yi Jiao ◽  
Dong Hoon Shin ◽  
...  
Keyword(s):  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Patch Clamp Recording ◽  
Cl Channel ◽  
Pacemaker Channels ◽  
Pacemaker Potentials ◽  
Small Intestinal ◽  
Cells Of Cajal ◽  
Ca2 Channels ◽  
Do So

Background/Aims: Anoctamin1 (Ca2+-activated Cl- channel, ANO1) is a specific marker of the interstitial cells of Cajal (ICC) in the gastrointestinal tract, and are candidate proteins that can function as pacemaker channels. Recently, novel selective ANO1 inhibitors were discovered and used to study Ca2+-activated Cl- channels. Therefore, to investigate whether ANO1 channels function as pacemaker channels, selective ANO1 inhibitors were tested with respect to the pacemaker potentials in ICC. Methods: Whole-cell patch-clamp recording, RT-PCR, and intracellular Ca2+ ([Ca2+]i) imaging were performed in cultured ICC obtained from mice. Results: Though CaCCinh-A01 (5 µM), T16Ainh-A01 (5 µM), and MONNA (5 µM) (selective ANO1 inhibitors) blocked the generation of pacemaker potentials in colonic ICC, they did not do so in small intestinal ICC. Though nifulmic acid (10 µM) and DIDS (10 µM) (classical Ca2+-activated Cl- channel inhibitors) also had no effect in small intestinal ICC, they suppressed the generation of pacemaker potentials in colonic ICC. In addition, knockdown of ANO1 reduced the pacemaker potential frequency in colonic ICC alone. Though ANO1 inhibitors suppressed [Ca2+]i oscillations in colonic ICC, they did not do so in small intestinal ICC. T-type Ca2+ channels were expressed in the both the small intestinal and colonic ICC, but mibefradil (5 µM) and NiCl2 (30 µM) (T-type Ca2+ channel inhibitors) inhibited the generation of pacemaker potentials in colonic ICC alone. Conclusion: These results indicate that though ANO1 and T-type Ca2+ channels participate in generating pacemaker potentials in colonic ICC, they do not do so in small intestinal ICC. Therefore, the mechanisms underlying pacemaking in ICC might be different in the small intestine and the colon.

Sodium current in human small intestinal interstitial cells of cajal

2001 ◽  
Vol 120 (5) ◽  
pp. A201-A201 ◽  
Author(s):  
P STREGE ◽  
A RICH ◽  
Y OU ◽  
S GIBBONS ◽  
M SARR ◽  
...  
Keyword(s):  
Interstitial Cells Of Cajal ◽  
Sodium Current ◽  
Interstitial Cells ◽  
Small Intestinal ◽  
Cells Of Cajal

Study of the ESI and APCI interfaces for the UPLC–MS/MS analysis of pesticides in traditional Chinese herbal medicine

2013 ◽  
Vol 406 (5) ◽  
pp. 1481-1491 ◽  
Author(s):  
Lina Chen ◽  
Fengrui Song ◽  
Zhiqiang Liu ◽  
Zhong Zheng ◽  
Junpeng Xing ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Ms Analysis
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