Multiphoton Microscopy for Caries Detection with ICDAS Classification

2018 ◽  
Vol 52 (5) ◽  
pp. 359-366 ◽  
Author(s):  
Amel Slimani ◽  
Delphine Tardivo ◽  
Ivan V. Panayotov ◽  
Bernard Levallois ◽  
Csilla Gergely ◽  
...  
Keyword(s):  
Nonlinear Optical ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Organic Matrix ◽  
Collagen Type I ◽  
Assessment System ◽  
Caries Detection ◽  
Major Protein ◽  
Type I ◽  
Carious Lesion

Dentin carious lesion is a dynamic process that involves demineralization and collagen denaturation. Collagen type I is the major protein in dentin and it has been investigated based on its optical properties. Multiphoton microscopy (MPM) is a nonlinear imaging technique that reveals the caries process using the collagen two-photon excitation fluorescence (2PEF) and its second-harmonic generation (SHG). Combining the histological and the International Caries Detection and Assessment System (ICDAS) classifications with nonlinear optical spectroscopy (NLOS), 2PEF and SHG intensities of enamel and dentin were highly altered during the caries process. It has been proven that the ratio SHG/2PEF is a relevant indicator of the organic matrix denaturation [Terrer et al.: J Dent Res 2016; 96: 574–579]. In the present study, a series of measurable signals is made to detect early stages of carious lesion according to the ICDAS classification and to explore the relationship between these measures and the ICDAS scale. Comparison of the efficiency of nonlinear optical signals for caries detection with the ICDAS classification is essential to evaluate their potential for clinical application. In our study, the use of the NLOS measured by MPM allowed us to monitor a quantitative parameter (SHG/2PEF ratio) according to the dentin carious lesion state (ICDAS and histological examination). Three coherent new groups were defined (ICDAS 0/1; ICDAS 2/3; ICDAS 4/5/6), where the carious process can be clearly described with a statistically significant decrease of the SHG/2PEF ratio.

scholarly journals Detection of Occlusal Carious Lesion using the SoproLife® Camera: A Systematic Review

2016 ◽  
Vol 17 (9) ◽  
pp. 774-779 ◽  
Author(s):  
Sophie Doméjean ◽  
Julie Rongier ◽  
Michèle Muller-Bolla
Keyword(s):  
Systematic Review ◽  
Scientific Evidence ◽  
Assessment System ◽  
Caries Detection ◽  
Permanent Teeth ◽  
Carious Lesion ◽  
Carious Lesions ◽  
Pubmed Database ◽  
Icdas Ii

ABSTRACT Aim The aim of this systematic review was to assess the in vivo scientific evidence regarding the ability of a recently developed light fluorescence device, SoproLife® (Sopro-Acteon group, La Ciotat, France) in detecting occlusal carious lesions. The PubMed database was searched for in vivo trials that evaluated the validity of the SoproLife® camera for the detection of occlusal carious lesions. Among the 11 articles originally identified with the keyword “Soprolife,” only three articles were included. The three included surveys used the International Caries Detection and Assessment System (ICDAS)-II criteria as gold standard for the assessment of SoproLife® compared or not to other detection devices (DIAGNOdent® and Spectra Caries Detection Aid®). Two of the included studies reported only on permanent teeth or both primary and permanent teeth. The SoproLife® validity values varied markedly among studies with a sensitivity ranging between 0.43 and 0.95 and a specificity between 0.55 and 1. Interobserver reproducibility with the SoproLife® was reported in two of the three studies (0.98 and 0.72) and none of the studies reported about intraobserver reproducibility. No clear-cut conclusion can be made based on the three included clinical studies; further in vivo investigations are needed to confirm the validity of the SoproLife® camera in terms of detection of occlusal carious lesions. How to cite this article Doméjean S, Rongier J, Muller-Bolla M. Detection of Occlusal Carious Lesion using the SoproLife® Camera: A Systematic Review. J Contemp Dent Pract 2016;17(9):774-779.

Li8NaRb3(SO4)6·2H2O as a new sulfate deep-ultraviolet nonlinear optical material

2018 ◽  
Vol 6 (45) ◽  
pp. 12240-12244 ◽  
Author(s):  
Yanqiang Li ◽  
Sangen Zhao ◽  
Pai Shan ◽  
Xianfeng Li ◽  
Qingran Ding ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Optical Material ◽  
Type I ◽  
Nonlinear Optical Material ◽  
Deep Ultraviolet

A new sulfate Li8NaRb3(SO4)6·2H2O exhibits a short cutoff edge below λ < 190 nm and has a moderate second-harmonic generation response in type-I phase-matchable behavior.

scholarly journals Mechanisms of development of alcohol-dependant osteoporosis

2012 ◽  
Vol 93 (1) ◽  
pp. 120-122
Author(s):  
D N Goryachev ◽  
L R Mukhamedzhanova
Keyword(s):  
Mutual Influence ◽  
Organic Matrix ◽  
Collagen Type I ◽  
Published Data ◽  
Type I ◽  
Characteristic Features ◽  
Chronic Ethanol Intoxication ◽  
Immune Pathology ◽  
Mechanisms Of Development

Presented were the current published data on the mutual influence of different factors on the initiation and progression of disorders of formation of the bone organic matrix and its mineralization during chronic ethanol intoxication. Emphasized was the role of the toxic effect of ethanol on the osteoblastic cells, which is expressed in the increasing viscosity of the cytoplasm, disruption of the architectonics of the cytoplasmic membrane, disorganization of the polyribosomal complexes and reduction of the collagen synthesis functions. Established was the role of immune pathology, which included the formation of antibodies to a number of autogenous tissues. Found were antibodies to collagen type I, which was modified by acetaldehyde and possessed cytotoxicity. In patients with alcohol dependence noted was an increase in the concentration of interleukin-6, which stimulates the early stages of hematopoiesis and osteoclastogenesis. The mechanism of development of alcohol-dependant osteoporosis is understood as a cascade of related processes, which are linked in the circulus vitiosus. Only the initial stages of this cascade have certain specificity. The morphological consequences are mostly similar and do not differ from those of other forms of secondary systemic osteoporosis, however give the characteristic features to the clinical course.

scholarly journals Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization

2020 ◽  
Author(s):  
Nirmal Mazumder ◽  
Fu-Jen Kao
Keyword(s):  
Skeletal Muscle ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Muscle Fiber ◽  
Collagen Type ◽  
Second Harmonic ◽  
Collagen Type I ◽  
Skeletal Muscle Fiber ◽  
Type I ◽  
Stokes Vector

Abstract The complete polarization state of second harmonic (SH) light was measured and characterized by collagen type I and skeletal muscle fiber using a Stokes vector-based SHG microscope. The polarization states of the SH signal are analyzed in a pixel-by-pixel manner and displayed through two dimensional (2D) Stokes vector images. Various polarization parameters are reconstructed using Stokes values to quantify the polarization properties of SH light. Also, the measurements are extended for different input polarization states to investigate the molecular structure of second harmonic generation (SHG) active molecules such as collagen type I and myosin.

scholarly journals Immunohistochemical distribution of collagens types IV, V, and VI and of pro-collagens types I and III in human alveolar bone and dentine.

1986 ◽  
Vol 34 (11) ◽  
pp. 1417-1429 ◽  
Author(s):  
J Becker ◽  
D Schuppan ◽  
H Benzian ◽  
T Bals ◽  
E G Hahn ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Collagen Type ◽  
Alveolar Bone ◽  
Organic Matrix ◽  
Collagen Type I ◽  
Basement Membranes ◽  
Type I ◽  
Collagen Type Iii ◽  
Type Iii ◽  
Hard Tissues

The aim of the present study was to characterize the composition of the organic matrix in alveolar jaw bone and dentine using antibodies against pro-collagens Types I and III and collagens Types IV, V, and VI. After demineralization of oral hard tissues in 0.2 N HCl, antigenicity was well preserved and the distribution of the pro-collagens and collagens could be demonstrated. Staining for pro-collagen Type I was prominent around osteoblasts and in pre-dentine, indicating active de novo synthesis of Type I pro-collagen. Pro-collagen Type I was ubiquitous but was less abundant in bone and dentine, whereas pro-collagen Type III was seen only in areas of bone remodeling, in peritubular spaces, and in pre-dentine. Type IV collagen was limited to the basement membranes of vessels in osteons and bone marrow. Type V collagen was detected neither in pre-dentine nor in bone. In contrast, Type VI collagen was found in dentine and bone, showing a faint but homogeneous staining which, similarly to pro-collagen Type III, was pronounced around osteoblasts and in pre-dentine, areas of active bone and dentine formation. This study showed that the organic matrix of dentine and bone contains Type VI as well as Type I collagen. Pro-collagen Type III (and to a lesser extent collagen Type VI) is transiently produced during new formation and remodeling of oral hard tissues, and disappears once the matrix calcifies. Type I pro-collagen qualifies as a general marker protein for increased osteoblastic activity. We conclude that immunostaining for the different collagen/pro-collagen types can be used to assess normal or abnormal stages of bone/dentine formation.

Quadratic Nonlinear Optical Properties of Diva Crystal

1989 ◽  
Vol 173 ◽  
Author(s):  
T. Wada ◽  
C. H. Grossman ◽  
S. Yamada ◽  
A. Yamada ◽  
A. F. Garito ◽  
...  
Keyword(s):  
Optical Properties ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Nonlinear Optical Properties ◽  
Crystallographic Structure ◽  
Type I ◽  
Sum Frequency ◽  
Fundamental Wavelength ◽  
Efficient Type ◽  
Light Generation

ABSTRACTNew organic nonlinear optical crystal : dicyanovinyl anisole (DIVA) has been grown from vapor by low pressure sublimation and from saturated solution by solvent evaporation. Crystallographic structure and quadratic nonlinear optical properties were investigated. Molecular orientation in DIVA crystal with space group P21, is favorable for the highest possible value of bulk phase-matchable coefficient. Both type I and type II phase matched second harmonic generation (PMSHG) were observed from naturally grown faces of single crystals. Efficient PMSHG was achieved at a fundamental wavelength of 812 and 1064 nm. The efficient type I PMSHG coefficient was determined as deff = 4.9 × 10−8 esu = 40 × (d11 of α-quartz) at a wave.LH length of 1064 nm. Blue light generation was demonstrated by both PMSHG and sum-frequency mixing of the 812 nm laser with 1064 nm.

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