scholarly journals The State of Intracellular Molecular Regulators during the Reconvalescence of Community-Acquired Pneumonia under the Influence of Microwaves at 1 GHz

2018 ◽  
Vol 4 (3-4) ◽  
pp. 171-180
Author(s):  
Andrey A. Voevodin ◽  
Alexander A. Khadartsev ◽  
Stanislav S. Bondar ◽  
Igor V. Terekhov ◽  
Victor S. Nikiforov
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
P53 Protein ◽  
Microwave Frequency ◽  
Community Acquired Pneumonia ◽  
Mononuclear Leukocytes ◽  
Research Results ◽  
Phosphorylated Form ◽  
Phosphorylation Level ◽  
Whole Blood Cells

We evaluated the levels of protein p53, retinoblastoma protein (RB), β-catenin, SMAD2, protein kinases AKT and focal adhesion kinase (FAK), and transcription factor CREB using ELISA in mononuclear leukocytes of patients with community-acquired pneumonia on days 15–17 of the disease. The research results showed that the subclinical immune-inflammatory process was characterized by a higher content of β-catenin by 19.8%, CREB by 23.3%, RB protein by 14.7%, increased phosphorylation level of protein kinase FAK by 19.8%, АKТ1 at serine-473 by 65.6%, and RB by 13.7%. In the cells, there was a decrease in p53 of 15.7%, SMAD2 of 16.9%, and protein kinase АKТ1 of 31.2%. Three hours after 1-GHz microwave irradiation, MNCs displayed a statistically significant increase in their content of the p53 protein of 20.0‰, RB of 9.35‰, β-catenin of 10.9‰, protein kinase FAK of 10.0‰, and CREB of 8.55‰. A day after a single irradiation of the cultures of whole blood cells, in irradiated cells compared to nonirradiated control, we observed a statistically significant increase in the content of p53 of 24.2‰, β-catenin of 15.1‰, SMAD2 of 20.4‰, RB of 9.8‰, as well as a reduction of the initially elevated levels of protein kinase AKT1 of 25.1‰, its phosphorylated form at serine-473 of 16.5‰, and a decrease in the phosphorylated form of the transcription factor CREB at serine-133 of 4.1‰. The research results indicate that a low-intensity microwave frequency of 1 GHz can be considered as a factor in molecular immune rehabilitation in conditions of community-acquired pneumonia.

scholarly journals The role of suppressor of cytokine signaling SOCS7 in the regulation of the phosphorylation of inhibitor of nuclear transcription factor NF-KB in mononuclear leukocytes and production of cytokines in community-acquired bacterial pneumonia

2018 ◽  
pp. 138-140
Author(s):  
S. S. Bondar ◽  
I. V. Terekhov ◽  
V. S. Nikiforov ◽  
V. K. Parfenyuk ◽  
N. V. Bondar
Keyword(s):  
Transcription Factor ◽  
Linear Regression Analysis ◽  
Negative Relationship ◽  
Adaptive Immune Response ◽  
Community Acquired Pneumonia ◽  
Cytokine Signaling ◽  
Mononuclear Leukocytes ◽  
Nuclear Transcription Factor ◽  
Factor Inhibitor

The study discusses the relationship between the content of NF-KB and cytokine signaling suppressor 7 (SOCS7) in mononuclear peripheral blood cells (MNC) phosphorylated form of nuclear transcription factor inhibitor (NF-KB) and the production of MNC cytokines (TNF, IFN, IL-1β, IL-4, IL-10, IL-12) determining the state of congenital and adaptive immune response.The content and level of phosphorylation of the nuclear transcription factor NF-KB (Ikba) inhibitor and the SOCS7 protein concentration were determined by enzyme immunoassay in MNC. In addition, the concentration of TNF, IFN, IL-1β, IL-4, IL-10, IL-12 was determined in cellular supernatants. The interrelations between the studied factors were evaluated by the method of linear regression analysis.The results of the study indicate that the stage of recovery of community-acquired pneumonia is accompanied by a decrease in the level of IL-1, TNF and IL-4 and an increase in the production of Information. Also in the stage of convalescence there is a decrease in phosphorylation of Ikba and an increase in the concentration of SOCS7 in the OLS. The analysis revealed a significant effect on the level of phosphorylation of Ikba content in the cell SOCS7. Thus, a strong negative relationship between SOCS7 and phosphorylation of Ikba can be mediated by inhibition under its influence of STAT3/5 and MARK / SAPK-dependent cytokine production mechanisms, which allows to consider this factor as a therapeutic target for limiting excessive immunosuppression in pneumonia.

scholarly journals THE RELATIONSHIP OF THIOL STATUS, AND COMPONENTS OF SIGNALING PATHWAYS THAT REGULATE INFLAMMATION IN CONVALESCENTS WITH COMMUNITY-ACQUIRED PNEUMONIA

2018 ◽  
Vol 8 (6) ◽  
pp. 451-457
Author(s):  
S. S. Bondar ◽  
I. V. Terekhov ◽  
V. K. Parfenyuk ◽  
N. V. Bondar ◽  
V. S. Nikiforov
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Signaling Pathways ◽  
Mononuclear Cells ◽  
Negative Relationship ◽  
Specific Effect ◽  
Community Acquired Pneumonia ◽  
Nuclear Transcription Factor ◽  
Relationship Of ◽  
The Relationship

The study discusses the relationship of thiol concentrations in intercellular fluid with the level of peripheral blood mononuclear cells (MNCs) in convalescents with community-acquired pneumonia (CAP) components MAPK/SAPK and JAK/STAT-signaling pathways, nuclear transcription factor NF-KB. The content and level of phosphorylation of JAK2 protein kinase, signal transducers and transcription activators STAT3, STAT5A, STAT6, NFKB nuclear transcription factor inhibitor (IkBa), stress-activated protein kinases JNK, ERK, the level of the p50 subunit of nuclear transcription factor NF-κB were determined by enzyme immunoassay in MNC. The results of the study indicate that the stage of reconvalescence of CAP is characterized by a lack of antioxidant protection, manifested by a decrease in the concentration of thiol compounds in the supernatant against which there is a decrease in the level of phosphorylation of protein kinase JAK2, factors STAT3, STAT5, STAT6, JNK, which is also associated with an increase in the level of phosphorylation of protein kinase ERK. The analysis showed that the thiol status is characterized by a positive relationship with the activity of STAT5A, JNK, p50. The level of thiols and ERK, as well as STAT3, was characterized by a negative relationship. Thus, the increase in the level of thiols contributes to the increase in the activity of the transcription factor STAT5A and decrease-STAT3 with a corresponding change in cell reactivity with respect to specific cytokines, as well as a specific effect on the differentiation of individual populations of immunocompetent cells.

scholarly journals Activation of a Novel Transcription Factor through Phosphorylation by WIPK, a Wound-Induced Mitogen-Activated Protein Kinase in Tobacco Plants

2005 ◽  
Vol 139 (1) ◽  
pp. 127-137 ◽  
Author(s):  
Yun-Kiam Yap ◽  
Yutaka Kodama ◽  
Frank Waller ◽  
Kwi Mi Chung ◽  
Hirokazu Ueda ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Tobacco Plants ◽  
Mitogen Activated Protein

scholarly journals The Basic Helix-Loop-Helix Transcription Factor Cph2 Regulates Hyphal Development in CandidaalbicansPartly via Tec1

2001 ◽  
Vol 21 (19) ◽  
pp. 6418-6428 ◽  
Author(s):  
Shelley Lane ◽  
Song Zhou ◽  
Ting Pan ◽  
Qian Dai ◽  
Haoping Liu
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Binding Sites ◽  
Regulatory Element ◽  
Ectopic Expression ◽  
Mitogen Activated Protein Kinase ◽  
Northern Analysis ◽  
Hyphal Development ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

ABSTRACT Candida albicans undergoes a morphogenetic switch from budding yeast to hyphal growth form in response to a variety of stimuli and growth conditions. Multiple signaling pathways, including a Cph1-mediated mitogen-activated protein kinase pathway and an Efg1-mediated cyclic AMP/protein kinase A pathway, regulate the transition. Here we report the identification of a basic helix-loop-helix transcription factor of the Myc subfamily (Cph2) by its ability to promote pseudohyphal growth inSaccharomyces cerevisiae. Like sterol response element binding protein 1, Cph2 has a Tyr instead of a conserved Arg in the basic DNA binding region. Cph2 regulates hyphal development in C. albicans, ascph2/cph2 mutant strains show medium-specific impairment in hyphal development and in the induction of hypha-specific genes. However, many hypha-specific genes do not have potential Cph2 binding sites in their upstream regions. Interestingly, upstream sequences of all known hypha-specific genes are found to contain potential binding sites for Tec1, a regulator of hyphal development. Northern analysis shows that TEC1 transcription is highest in the medium in which cph2/cph2 displays a defect in hyphal development, and Cph2 is necessary for this transcriptional induction of TEC1. In vitro gel mobility shift experiments show that Cph2 directly binds to the two sterol regulatory element 1-like elements upstream of TEC1. Furthermore, the ectopic expression of TEC1 suppresses the defect ofcph2/cph2 in hyphal development. Therefore, the function of Cph2 in hyphal transcription is mediated, in part, through Tec1. We further show that this function of Cph2 is independent of the Cph1- and Efg1-mediated pathways.

scholarly journals Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

2018 ◽  
Vol 13 (1) ◽  
pp. 431-445 ◽  
Author(s):  
Wei Tang ◽  
Caroline Luo
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Salt Stress Tolerance ◽  
Dependent Protein Kinase ◽  
Zinc Finger Transcription Factor ◽  
Transgenic Cell ◽  
Dependent Protein

AbstractThe purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of Arabidopsis thaliana 6 (ZAT6) in salt stress tolerance in cells of rice (Oryza sativa L.), cotton (Gossypium hirsutum L.) and slash pine (Pinus elliottii Engelm.). Cells of O. sativa, G. hirsutum, and P. elliottii overexpressing ZAT6 were generated using Agrobacterium-mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of ZAT6 increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA8, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, ZAT6 also increased expression of Ca2+-dependent protein kinase genes OsCPK9 and OsCPK25 by 5–7 fold under NaCl stress. Altogether, our results suggest that overexpression of ZAT6 enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca2+-dependent protein kinase in transgenic cell lines of different plant species.

σF, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-σ factor that is also a protein kinase

Cell ◽  
1993 ◽  
Vol 74 (4) ◽  
pp. 735-742 ◽  
Author(s):  
Kyung-Tai Min ◽  
Corinne M. Hilditch ◽  
Bettina Diederich ◽  
Jeffery Errington ◽  
Michael D. Yudkin
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Specific Transcription Factor ◽  
Σ Factor
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