Higher Thrombin-Antithrombin III Complex Levels May Indicate Severe Acute Pancreatitis

2018 ◽  
Vol 36 (3) ◽  
pp. 244-251 ◽  
Author(s):  
Sami Fidan ◽  
Murat Erkut ◽  
Arif Mansur Cosar ◽  
Yasar Yogun ◽  
Asım Örem ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Antithrombin Iii ◽  
Coagulation Cascade ◽  
Control Group ◽  
Coagulation Disorder ◽  
Healthy Control ◽  
Severity Of The Disease ◽  
Time Of Presentation ◽  
Thrombin Antithrombin Iii Complex

Aim: Coagulation disorders may develop in association with severe acute pancreatitis (AP). Plasma thrombin-antithrombin III complex (TAT) levels are one of the principal markers of coagulation disorder. The purpose of this study was to evaluate TAT and other hemostatic parameters in patients with AP and to examine whether or not these parameters indicate the severity of AP. Method: Forty-six patients with AP (14 severe, 32 non-severe) and a 30-member healthy control group were recruited. The severity of AP was determined using the revised Atlanta classification. ELISA was used to measure patients’ plasma TAT levels. Results: The TAT levels of AP patients at presentation were higher than those of the control group (p = 0.005). The plasma TAT levels of patients with severe AP were also significantly higher than those of patients with non-severe AP (p = 0.05) and of the control group (p < 0.001). The general accuracy, sensitivity and specificity of TAT levels in predicting the severity of AP were 77.4, 77.8, and 77.3% respectively. Conclusion: The coagulation cascade was activated in the AP patients in our study, and this was shown to become more pronounced as severity of the disease increased. Plasma TAT levels at the time of presentation in patients with AP can be used as a marker for predicting the severity of the disease.

Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells (huMSCs) Carrying MicroRNA-342 Relieve Protect Severe Acute Pancreatitis Injury (SAP)

2021 ◽  
Vol 11 (9) ◽  
pp. 1838-1843
Author(s):  
Xiaohong Zhou ◽  
Xuzhong Hao ◽  
Feifei He
Keyword(s):  
Stem Cells ◽  
Acute Pancreatitis ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Severe Acute Pancreatitis ◽  
Pancreatic Tissue ◽  
Inflammatory Factors ◽  
Control Group ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord

To investigate whether exosomes (exo) derived from human umbilical cord mesenchymal stem cells (huMSCs) and microRNA (miRNA)-342 have a protective effect on severe acute pancreatitis (SAP). Human umbilical cord blood was collected to extract huMSC-exo. With sham-operated mice as control group (n = 10), the other mice were induced to SAP model (n = 20), while 10 of the SAP mice received treatment with huMSC-exo. ELISA was performed to determine amylase and TAP level as well as inflammatory factors and HE staining to evaluate pathological changes of pancreatic tissue. The expression of miR-342 and Shh, Ptchl, and Smo in the Hh signal pathway was detected using RT-qPCR. The expression of miR-342 and the mRNA expression of Shh, Ptchl, and Smo was higher than that in model group (p < 0.05). The level of serum amylase, trypsinogen, and IFN-γ,Fasl, and IL-6 was upregulated in pancreas tissues of SAP mice relative to healthy mice, but their levels were decreased upon treatment with huMSC-exo and slightly higher than those of the control group, just not significantly. Collectively, the huMSC-exo may activate the Hh signaling pathway by regulating the expression of miR-342 increasing the expression of Shh, Ptchl, and Smo, and thereby healing of damaged pancreatic tissues in SAP.

scholarly journals Infusion of Bone Marrow Mesenchymal Stem Cells Attenuates Experimental Severe Acute Pancreatitis in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Hang Zhao ◽  
Zhiying He ◽  
Dandan Huang ◽  
Jun Gao ◽  
Yanfang Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Acute Pancreatitis ◽  
Mesenchymal Stem Cells ◽  
Survival Rate ◽  
Severe Acute Pancreatitis ◽  
Control Group ◽  
Therapeutic Effects ◽  
Isolation And Characterization ◽  
Marrow Mesenchymal Stem Cells

Background & Aims. Severe acute pancreatitis (SAP) remains a high-mortality disease. Bone marrow (BM) mesenchymal stem cells (MSCs) have been demonstrated to have plasticity of transdifferentiation and to have immunomodulatory functions. In the present study, we assessed the roles of MSCs in SAP and the therapeutic effects of MSC on SAP after transplantation.Methods. A pancreatitis rat model was induced by the injection of taurocholic acid (TCA) into the pancreatic duct. After isolation and characterization of MSC from BM, MSC transplantation was conducted 24 hrs after SAP induction by tail vein injection. The survival rate was observed and MSCs were traced after transplantation. The expression of TNF-αand IL-1βmRNA in the transplantation group was also analyzed.Results. The survival rate of the transplantation group was significantly higher compared to the control group (p<0.05). Infused MSCs were detected in the pancreas and BM 3 days after transplantation. The expression of TNF-αand IL-1βmRNA in the transplantation group was significantly lower than in the control group in both the pancreas and the lungs (p<0.05).Conclusions. MSC transplantation could improve the prognosis of SAP rats. Engrafted MSCs have the capacity of homing, migration, and planting during the treatment of SAP.

scholarly journals C5n 5 55Detection of Some Immunological Parameters in Psoriatic Iraqi Female Patients

2020 ◽  
pp. 2515-2524
Author(s):  
Rehab Morad Khazem ◽  
Shaima R. Ibraheem
Keyword(s):  
Serum Levels ◽  
Interleukin 10 ◽  
Living Condition ◽  
Healthy Control Group ◽  
Control Group ◽  
Immunological Parameters ◽  
Female Patients ◽  
Healthy Control ◽  
The Mean ◽  
Severity Of The Disease

Psoriasis is a common, chronic, immune-mediated skin disease with systemic pro-inflammatory activation.  This study was designed to estimate the level of two cytokines, Interleukin-36 (IL-36) and Interleukin-10 (IL-10), in psoriasis female patients. The study was accomplished on 50 Iraqi patients with psoriasis who were referred to the consulting clinic at Al-Yarmouk Teaching Hospital during the period from November 2018 to March 2019. These patients were diagnosed under the supervision of dermatologists. For the purpose of comparison, the study included 30 healthy women as a healthy control group. The serum levels of cytokines  were measured using the enzyme-linked immunosorbent technique (ELISA).The results of this study showed that the mean age of the female patients was 35.9 ± 1.85 years, whereas the age of the patients with a severity of higher than 30% ranged 15-25 years. Most of the patients were married, in an average living condition, and non-smokers, and their menstrual cycle was continuous. It was also found that 28% of the psoriatic patients had other chronic diseases. The study showed statistically significant differences (p <0.05) in the mean level of IL-36 between the patients and healthy control group, whereas there was no statistical difference in the mean level of IL-10. In conclusion,   the   decrease in the level of IL-36 in the patients might be related to the increase in the severity of the disease.

scholarly journals The effect of enteral nutrition on intra-abdominal pressure in severe acute pancreatitis patients

2017 ◽  
Author(s):  
Mingjie Zhang ◽  
Li-Ping Cao ◽  
Guoping Ding
Keyword(s):  
Acute Pancreatitis ◽  
Enteral Nutrition ◽  
Severe Acute Pancreatitis ◽  
Occurrence Rate ◽  
Control Group ◽  
Abdominal Pressure ◽  
Therapeutic Effects ◽  
Study Group ◽  
Time Operation ◽  
Operation Rate

Abstract BACKGROUND: To investigate the effect of enteral nutrition (EN) on intra-abdominal pressure (IAP) in severe acute pancreatitis (SAP) patients and the relationship between the decreasion of IAP and the the therapeutic effect of EN. METHODS: Eighty SAP patients were randomly divided into study group and control group (40 patients in each group). Patients in the study group received EN and Patients in the control group received total parenteral nutrition (TPN) for 7 days. Intra-cystic pressure (ICP) of the two groups was measured during treatment period. The outcomes of treatment were observed, APACHE Ⅱ scores, NB data were applied in analysis. RESULTS: The ICP data was lower in the study group than in the control group on days 4 and 5 of treatment (P &lt; 0.05). On days 3-5 of treatment, the APACHE Ⅱ scores of the study group were lower than which of the control group (P &lt; 0.05). Nitrogen balance (NB) date increased significantly in study group than in the control group (P &lt; 0.05). abdominalgia relief time, operation rate were different between the two groups (P &lt; 0.05). The abdominal compartment syndrome (ACS) occurrence rate of the two groups have no significant difference. The ICP data and APACHE Ⅱscores, abdominalgia relief time, operation rate of pancreas debridement show positive correlation (P &lt; 0.05). ICP and NB date show negative correlation (P &lt; 0.05). CONCLUSION: EN can decrease the IAP of SAP, which may be the reason for EN show preventive and therapeutic effects on SAP.

Urinary Excretion Rate of Antithrombin III Related Antigen in Cerebral Stroke

1984 ◽  
Vol 52 (03) ◽  
pp. 305-307 ◽  
Author(s):  
T Matsuo ◽  
K Shinomiya ◽  
S Okuno ◽  
C Matsuo ◽  
O Matsuo
Keyword(s):  
Urinary Excretion ◽  
Cerebral Hemorrhage ◽  
Antithrombin Iii ◽  
Excretion Rate ◽  
Control Group ◽  
Cerebral Stroke ◽  
Cerebral Thrombosis ◽  
Urinary Excretion Rate ◽  
At Iii ◽  
Healthy Control

SummaryThe urinary excretion rate of antithrombin III related antigen (AT III RA) was examined in cerebral stroke. The excretion rate of AT III RA in cerebral hemorrhage (CH) was 12.33 ± 1.61 × 10-4 ml/min. The patients with CH were further classified into two groups: in group CH-I, whose consciousness state was stupor or further deteriorated including coma on admission, the excretion rate of AT III RA was 18.08 ± 2.50 × 10-4 ml/min. In group CH-II, whose consciousness state was clear on admission, the excretion rate of AT III RA was significantly lower than that in CH-I (6.20 ± 1.56 × 10-4 ml/min). The excretion rate in cerebral thrombosis (CT) was 1.96 ± 0.25 × 10-4 ml/min, which was significantly lower than that in CH. The excretion rate of AT III RA in both CH and CT was significantly higher than that in the healthy control group (0.29 ± 0.04 × 10-4 ml/min). Thus, AT III may change dynamically in cerebral stroke.

scholarly journals Effect of intra-abdominal infection on immunological function and HMGB1/TLR4/NF-κB signaling pathway in patients with severe acute pancreatitis

2018 ◽  
Vol 16 ◽  
pp. 205873921882022
Author(s):  
Cuihong Qin ◽  
Ya Li ◽  
Ruifeng Song ◽  
Feng Xu
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Diamine Oxidase ◽  
Apache Ii Score ◽  
Control Group ◽  
Immunological Function ◽  
Infection Group ◽  
Abdominal Infection ◽  
Tnf Α ◽  
Intra Abdominal Infection

The aim of this article is to investigate the effects of intra-abdominal infection on immunological function and high-mobility group box 1 protein (HMGB1)/toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) pathway in patients with severe acute pancreatitis (SAP). Clinical data of SAP patients were retrospectively analyzed. SAP patients were divided into intra-abdominal infection group (103 SAP patients) and control group (115 SAP patients without intra-abdominal infection). All patients were evaluated with the Acute Physiology and Chronic Health Evaluation (APACHE) II score. Enzyme-linked immunosorbent assay (ELISA) assays were used to detect the levels of serum endotoxin, d-lactate, diamine oxidase, IgG, IgM, IgA, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and HMGB1. Western blotting was performed to detect the levels of TLR4 and NF-κB in peripheral blood lymphocytes. Compared with control group, the APACHE II score (12.60 ± 3.81 vs 9.55 ± 3.02) and serum endotoxin (0.33 ± 0.15 vs 0.19 ± 0.09 EU/mL), d-lactate (4.33 ± 0.16 vs 4.02 ± 0.12 mg/L), and diamine oxidase (3.88 ± 0.16 vs 3.65 ± 0.13 EU/mL) levels in intra-abdominal infection group were increased significantly (all P < 0.001); serum IgG (7.33 ± 0.82 vs 9.05 ± 0.90 g/L), IgM (1.04 ± 0.49 vs 1.18 ± 0.53 g/L), and IgA (1.65 ± 0.79 vs 1.96 ± 0.88 g/L) levels in intra-abdominal infection group were decreased significantly, while serum IL-1β (118.55 ± 17.04 vs 83.61 ± 12.28 ng/L), IL-6 (12.05 ± 7.69 vs 9.89 ± 6.77 ng/L), TNF-α (25.61 ± 8.76 vs 19.20 ± 8.33 ng/L), and HMGB1 (48.91 ± 20.63 vs 32.74 ± 17.05 μg/L) levels were increased significantly (all P < 0.05); TLR4 and NF-κB in intra-abdominal infection group were increased significantly (both P < 0.001). The intra-abdominal infection can lead to intestinal barrier dysfunction, aggravated inflammatory response, and immune dysfunction in SAP patients, which may be related to the activation of HMGB1/TLR4/NF-κB pathway caused by intra-abdominal infection.

scholarly journals Elevated serum neopterin concentration increases mortality risk in patients with acute pancreatitis

Pteridines ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 16-20
Author(s):  
Zemin Xiang ◽  
Yijun Gu ◽  
Yuanyuan Huang ◽  
Lefeng Zhang ◽  
Xiaohong Zhang ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Mortality Risk ◽  
Elevated Serum ◽  
Multiple Organ ◽  
Control Group ◽  
Serum Neopterin ◽  
Severity Of Disease ◽  
Significant Difference ◽  
Mild Acute Pancreatitis

Abstract Background The aim of this study was to investigate serum neopterin levels in patients with acute pancreatitis (AP) and its predictive value for mortality in cases of severe acute pancreatitis (SAP). Methods Eighty-two patients with confirmed acute pancreatitis (AP) were included and divided into two groups, mild acute pancreatitis (MAP, n=51) and severe acute pancreatitis (SAP, n=31) according to severity of disease. From the SAP group, 8 cases went on to develop multiple organ dysfunction syndrome (MODS) and 6 subsequently died. Thirty healthy subjects from routine medical examination were included as the control group. The neopterin serum concentrations were determined and compared between groups. Results Serum neopterin concentrations from control, MAP, SAP, SAP_Non-MODS, MODS_survival and MODS_death groups were 6.85±2.42 (nmol/L), 0.91±4.83(nmol/L), 33.11±11.67(nmol/L), 30.39±9.97(nmol/L), 36.40±4.48(nmol/L) and 41.75±15.64(nmol/L) respectively, with statistical significant difference (p<0.05). The sensitivity and specificity for mortality risk were 66.67% (95%CI:22.28-95.67%) and 88.00%(95%CI:68.78-97.45%) respectively, with area under the ROC curve (AUC) of 0.71(95%CI:0.50-0.9), under the cut off value of 40.18. Conclusion: Serum neopterin levels in patients with acute pancreatitis were significantly elevated and correlated with the severity of disease. Neopterin may also be used a serological biomarker of mortality risk in patients with SAP.

Effects of Micro-Ecological Enteral Nutrition on Systemic Inflammatory Response, Bacterial Translocation, and Immune Function in Patients with Severe Acute Pancreatitis

2020 ◽  
Vol 19 (3) ◽  
pp. 240-247
Author(s):  
Yilan Wang ◽  
Hongwei Ye ◽  
Feng Zheng ◽  
Xinsen Zou ◽  
Xianbin Wu ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Enteral Nutrition ◽  
Inflammatory Response ◽  
Immune Function ◽  
Severe Acute Pancreatitis ◽  
Bacterial Translocation ◽  
Severity Index ◽  
Systemic Inflammatory Response ◽  
Mucosal Barrier ◽  
Control Group

We have evaluated the effect of early and late micro-ecological enteral nutrition on systemic inflammatory response, bacterial translocation, and immune function in patients with severe acute pancreatitis. Two weeks after treatment, experimental group receiving early nutritional intervention exhibited a significant increase in intestinal lactobacilli and bifidobacteria, fewer staphylococci, and E. coli, and lower levels of serum endotoxin, D-lactic acid, and diamine oxidase than the group receiving late intervention (control group) (P < 0.05). Also, the serum levels of CD3+ and CD4+ and CD4+/CD8+ ratio significantly increase after 2 weeks of intervention. On the other hand, the level of CD8+ and the Acute Physiology and Chronic Health Evaluation II and Modified Computed Tomography Severity Index scores significantly decreased after 2 weeks of treatment (P < 0.05). The early intervention also led to a significant shortening of time needed for abdominal pain relief, anal exhaustion, resumption of peristaltic sound, and hospitalization. Furthermore, there was a significant increase in overall response rate, and decrease in the incidence rate of complications (P < 0.05). Early micro-ecological enteral nutrition therapy can effectively relieve systemic inflammatory response, prevent intestinal bacterial translocation, restore the function of intestinal mucosal barrier, and alleviate nutritional imbalance in severe acute pancreatitis patients leading to improved immune function, mitigation of the disease, and reduced complications benefiting the recovery of patients.

scholarly journals Diaphragmatic dysfunction in sepsis due to severe acute pancreatitis complicated by intra-abdominal hypertension

2018 ◽  
Vol 46 (4) ◽  
pp. 1349-1357 ◽  
Author(s):  
Wei-Chao Liao ◽  
Yan-Hong Chen ◽  
Hang-Yang Li ◽  
Ting-Ting Wang ◽  
Peng Lan ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Rat Model ◽  
Contractile Function ◽  
Control Group ◽  
Force Output ◽  
Diaphragmatic Dysfunction ◽  
Abdominal Hypertension ◽  
And Control

Objective This study aimed to examine the mechanism of diaphragmatic dysfunction in sepsis due to severe acute pancreatitis (SAP) with intra-abdominal hypertension (IAH) in a rat model. Methods The rats were assigned at random to four groups: (1) control (n = 5), (2) SAP (n = 5), (3) SAP+IAH (n = 5), and (4) SAP+IAH+SS-31 (n = 5). Length and force output of the diaphragm were analysed in vivo. Histopathological examinations were performed by haematoxylin–eosin. Oxidative stress levels related to protease in diaphragmatic mitochondria were detected with a colorimetric technique. Results In the septic rat model due to SAP complicated by IAH, myofibres were increased. Muscle contractile function was significantly lower in the SAP+IAH group compared with the SAP and control groups. Glutathione peroxidase and superoxide dismutase levels were significantly lower and malondialdehyde levels were higher in the SAP and SAP+IAH groups compared with the control group. Notably, SS-31 could reverse atrophy of myofibres in SAP+IAH rats, as well as contractile dysfunction and mitochondrial dysfunction in the diaphragm. Conclusions Diaphragmatic structure and biomechanics are altered in septic rats due to SAP and IAH. This finding is mainly due to an increase in release of mitochondrial reactive oxygen species.

scholarly journals Combined effect of ultrasound-guided percutaneous abdominal paracentesis drainage and ulinastatin on severe acute pancreatitis

2021 ◽  
Vol 20 (10) ◽  
pp. 2187-2192
Author(s):  
Chuanmin Liu ◽  
Fei Yuan ◽  
Baowei Wang ◽  
Xuezhen Wang
Keyword(s):  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Study Groups ◽  
Control Group ◽  
Amylase Level ◽  
Study Group ◽  
Research Subjects ◽  
Ultrasound Guided ◽  
Beneficial Effects ◽  
Abdominal Paracentesis

Purpose: To study the clinical effect of a combination of ultrasound-guided percutaneous abdominal paracentesis drainage (APD) and ulinastatin on severe acute pancreatitis (AP).Methods: A total of 94 patients with severe AP in Intensive Care Unit, Jiaozhou Central Hospital, Qingdao, from December 2017 to December 2018 were selected as the research subjects. They were divided into control and study groups, with 47 patients in each group. Patients in the control group underwent laparotomy drainage, while patients in the study group underwent ultrasound-guidedpercutaneous APD. Patients in both groups received ulinastatin perfusion. Subsequently, clinical effectsand other relevant indicators were determined.Results: Overall response was significantly higher in the study group than in the control group (p < 0.05). The times taken for disappearance of postoperative symptoms, normalization of serum amylase level, and hospitalization were significantly shorter in the study group than in the control group (p < 0.05). For every indicator, the study group exhibited more benefits after than before treatment; however, post-treatment levels of blood glucose, hemodiastase and urinary amylase were better than those in the control group (p < 0.05). Incidence of postoperative complications was lower in the study group than in control group (p < 0.05).Conclusion: The combination of ultrasound-guided percutaneous APD with ulinastatin produces marked beneficial effects on severe AP patients. It facilitates the remission of adverse symptoms, and enhances  normalization of indicator levels. Moreover, it displays low incidence of complications, better prognosis and recovery, and absence of post-operation infections.

Sign in / Sign up

Export Citation Format

Share Document