A New Baltic Population-Specific Human Genetic Marker in the PMCA4 Gene

2016 ◽  
Vol 82 (3-4) ◽  
pp. 140-146
Author(s):  
Janis Stavusis ◽  
Inna Inashkina ◽  
Baiba Lace ◽  
Dita Pelnena ◽  
Svetlana Limborska ◽  
...  
Keyword(s):  
Genetic Marker ◽  
Baltic Population

scholarly journals Development of a male specific genetic marker for Garcinia subelliptica Merr. tree

2021 ◽  
pp. 1-8 ◽  
Author(s):  
Ayaka Irei ◽  
Matin Miryeganeh ◽  
Masanori Tamashiro ◽  
Hidetoshi Saze ◽  
Naoya Urasaki ◽  
...  
Keyword(s):  
Genetic Marker ◽  
Male Specific

scholarly journals Impact of gamma irradiation pretreatment on biochemical and molecular responses of potato growing under salt stress

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Elhamahmy Ali Mohamed ◽  
Elsadany Osama ◽  
Eid Manal ◽  
Abdelazeem Samah ◽  
Gerish Salah ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Genetic Marker ◽  
Gamma Rays ◽  
Salt Resistance ◽  
Base Pairs ◽  
Potato Genotype ◽  
Potato Callus ◽  
Primer Sequence ◽  
Significant Increment

Abstract Background Previous literatures revealed that gamma rays have an increasing effect on salt tolerance in different plants. In vitro experiment was conducted to study the effect of gamma rays (20 Gray) on salt tolerance of four potato cultivars (Lady Rosetta, Diamante, Gold, and Santana). Results Gamma-treated Santana plantlets were more tolerant to salinity as compared to other cultivars. It showed a significant increment of fresh weight (250% over the untreated). Gamma-treated plantlets of Lady Rosetta, Diamante, and Gold showed higher activity of peroxidase (POD) and polyphenol oxidase (PPO). Isoenzymes analysis showed an absence of POD 3, 4, and 5 in Gold plantlets. The dye of most PODs and PPOs bands were denser (more active) in gamma-treated plantlets of Santana as compared to other cultivars. Both gamma-treated and untreated plantlets showed the absence of PPO1 in Lady Rosetta and Diamante, and PPO 3, 4, and 5 in Gold plantlets. Genetic marker analysis using ISSR with six different primers showed obvious unique negative and positive bands with different base pairs in mutant plantlets as compared to the control, according to primer sequence and potato genotype. The 14A primer was an efficient genetic marker between mutated and unmutated potato genotypes. Santana had a unique fingerprint in the 1430-pb site, which can be a selectable marker for the cultivar. An increment in genetic distance between Gold cultivar and others proved that the mutation was induced because of gamma rays. Conclusion We assume that irradiation of potato callus by 20-Gy gamma rays is an effective process for inducing salt resistance. However, this finding should be verified under field conditions. Graphic Abstract

scholarly journals Lachnospiraceae and Bacteroidales Alternative Fecal Indicators Reveal Chronic Human Sewage Contamination in an Urban Harbor

2011 ◽  
Vol 77 (19) ◽  
pp. 6972-6981 ◽  
Author(s):  
Ryan J. Newton ◽  
Jessica L. VandeWalle ◽  
Mark A. Borchardt ◽  
Marc H. Gorelick ◽  
Sandra L. McLellan
Keyword(s):  
Sequence Analysis ◽  
Genetic Marker ◽  
Microbial Community Composition ◽  
Fold Increase ◽  
Fecal Pollution ◽  
Plate Count ◽  
Rrna Gene ◽  
Fecal Indicators ◽  
Fecal Indicator ◽  
Content Type

ABSTRACTThe complexity of fecal microbial communities and overlap among human and other animal sources have made it difficult to identify source-specific fecal indicator bacteria. However, the advent of next-generation sequencing technologies now provides increased sequencing power to resolve microbial community composition within and among environments. These data can be mined for information on source-specific phylotypes and/or assemblages of phylotypes (i.e., microbial signatures). We report the development of a new genetic marker for human fecal contamination identified through microbial pyrotag sequence analysis of the V6 region of the 16S rRNA gene. Sequence analysis of 37 sewage samples and comparison with database sequences revealed a human-associated phylotype within theLachnospiraceaefamily, which was closely related to the genusBlautia. This phylotype, termed Lachno2, was on average the second most abundant fecal bacterial phylotype in sewage influent samples from Milwaukee, WI. We developed a quantitative PCR (qPCR) assay for Lachno2 and used it along with the qPCR-based assays for humanBacteroidales(based on the HF183 genetic marker), totalBacteroidalesspp., and enterococci and the conventionalEscherichia coliand enterococci plate count assays to examine the prevalence of fecal and human fecal pollution in Milwaukee's harbor. Both the conventional fecal indicators and the human-associated indicators revealed chronic fecal pollution in the harbor, with significant increases following heavy rain events and combined sewer overflows. The two human-associated genetic marker abundances were tightly correlated in the harbor, a strong indication they target the same source (i.e., human sewage). Human adenoviruses were routinely detected under all conditions in the harbor, and the probability of their occurrence increased by 154% for every 10-fold increase in the human indicator concentration. Both Lachno2 and humanBacteroidalesincreased specificity to detect sewage compared to general indicators, and the relationship to a human pathogen group suggests that the use of these alternative indicators will improve assessments for human health risks in urban waters.

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