scholarly journals A Case of Adenocarcinoma of the Retinal Pigment Epithelium: An Immunohistochemical and Electron Microscopic Study

2017 ◽  
Vol 4 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Hidetsugu Mori ◽  
Kanji Takahashi
Keyword(s):  
Retinal Pigment Epithelium ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Electron Microscopic

Bipolar assembly of caveolae in retinal pigment epithelium

2006 ◽  
Vol 290 (3) ◽  
pp. C832-C843 ◽  
Author(s):  
Rosalia C. Mora ◽  
Vera L. Bonilha ◽  
Bo-Chul Shin ◽  
Jane Hu ◽  
Leona Cohen-Gould ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Epithelial Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Primary Cultures ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Caveolin 1 ◽  
Basolateral Side

Caveolae and their associated structural proteins, the caveolins, are specialized plasmalemmal microdomains involved in endocytosis and compartmentalization of cell signaling. We examined the expression and distribution of caveolae and caveolins in retinal pigment epithelium (RPE), which plays key roles in retinal support, visual cycle, and acts as the main barrier between blood and retina. Electron microscopic observation of rat RPE, in situ primary cultures of rat and human RPE and a rat RPE cell line (RPE-J) demonstrated in all cases the presence of caveolae in both apical and basolateral domains of the plasma membrane. Caveolae were rare in RPE in situ but were frequent in primary RPE cultures and in RPE-J cells, which correlated with increased levels in the expression of caveolin-1 and -2. The bipolar distribution of caveolae in RPE is striking, as all other epithelial cells examined to date (liver, kidney, thyroid, and intestinal) assemble caveolae only at the basolateral side. This might be related to the nonpolar distribution of both caveolin-1 and 2 in RPE because caveolin-2 is basolateral and caveolin-1 nonpolar in other epithelial cells. The bipolar localization of plasmalemmal caveolae in RPE cells may reflect specialized roles in signaling and trafficking important for visual function.

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