Purine de novo Synthesis in Liver and Developing Rat Brain, and the Effect of Some Inhibitors of Purine Nucleotide Inter con version

Enzyme ◽  
1983 ◽  
Vol 30 (3) ◽  
pp. 172-180 ◽  
Author(s):  
Jennifer Allsop ◽  
Richard W. E. Watts
Keyword(s):  
Rat Brain ◽  
De Novo ◽  
Purine Nucleotide ◽  
De Novo Synthesis ◽  
Developing Rat

Regulation of purine nucleotide biosynthesis: in yeast and beyond

2006 ◽  
Vol 34 (5) ◽  
pp. 786-790 ◽  
Author(s):  
R.J. Rolfes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
De Novo ◽  
Purine Nucleotide ◽  
De Novo Synthesis ◽  
Normal Functioning ◽  
Purine Nucleotides ◽  
Yeast Saccharomyces Cerevisiae ◽  
Nucleotide Biosynthesis ◽  
Salvage Pathways ◽  
Pool Sizes

Purine nucleotides are critically important for the normal functioning of cells due to their myriad of activities. It is important for cells to maintain a balance in the pool sizes of the adenine-containing and guanine-containing nucleotides, which occurs by a combination of de novo synthesis and salvage pathways that interconvert the purine nucleotides. This review describes the mechanism for regulation of the biosynthetic genes in the yeast Saccharomyces cerevisiae and compares this mechanism with that described in several microbial species.

De novo biosynthesis of nucleotides and of nucleic acids in different regions of developing rat brain: Effect of undernutrition

1982 ◽  
Vol 8 (1) ◽  
pp. 105-112 ◽  
Author(s):  
I. Serra ◽  
A. Vanella ◽  
M. Avitabile ◽  
M. L. Barcellona ◽  
R. Avola ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Rat Brain ◽  
De Novo ◽  
De Novo Biosynthesis ◽  
Developing Rat

scholarly journals Recycling of Carbon into Lipids Synthesized De Novo Is a Quantitatively Important Pathway of α-[U-13C]Linolenate Utilization in the Developing Rat Brain

2002 ◽  
Vol 71 (5) ◽  
pp. 2151-2158 ◽  
Author(s):  
Chantale R. Menard ◽  
Keith J. Goodman ◽  
Thomas N. Corso ◽  
J. Thomas Brenna ◽  
Stephen C. Cunnane
Keyword(s):  
Rat Brain ◽  
De Novo ◽  
Important Pathway ◽  
Developing Rat

scholarly journals De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues

2019 ◽  
Vol 294 (36) ◽  
pp. 13464-13477 ◽  
Author(s):  
Teresa W. M. Fan ◽  
Ronald C. Bruntz ◽  
Ye Yang ◽  
Huan Song ◽  
Yelena Chernyavskaya ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
De Novo ◽  
Human Lung Cancer ◽  
Purine Nucleotide ◽  
De Novo Synthesis ◽  
Nucleotide Biosynthesis ◽  
Cancer Tissues

Characterization of Monocyte-Associated Factor V

1993 ◽  
Vol 70 (02) ◽  
pp. 273-280 ◽  
Author(s):  
Janos Kappelmayer ◽  
Satya P Kunapuli ◽  
Edward G Wyshock ◽  
Robert W Colman
Keyword(s):  
Monoclonal Antibody ◽  
Light Chain ◽  
Peripheral Blood ◽  
De Novo ◽  
Factor V ◽  
Blood Monocytes ◽  
De Novo Synthesis ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Peripheral Blood Monocytes

SummaryWe demonstrate that in addition to possessing binding sites for intact factor V (FV), unstimulated peripheral blood monocytes also express activated factor V (FVa) on their surfaces. FVa was identified on the monocyte surface by monoclonal antibody B38 recognizing FVa light chain and by human oligoclonal antibodies H1 (to FVa light chain) and H2 (to FVa heavy chain) using immunofluorescence microscopy and flow cytometry. On Western blots, partially cleaved FV could be identified as a 220 kDa band in lysates of monocytes. In addition to surface expression of FVa, monocytes also contain intracellular FV as detected only after permeabilization by Triton X-100 by monoclonal antibody B10 directed specifically to the Cl domain not present in FVa. We sought to determine whether the presence of FV in peripheral blood monocytes is a result of de novo synthesis.Using in situ hybridization, no FV mRNA could be detected in monocytes, while in parallel control studies, factor V mRNA was detectable in Hep G2 cells and CD18 mRNA in monocytes. In addition, using reverse transcriptase and the polymerase chain reaction, no FV mRNA was detected in mononuclear cells or in U937 cells, but mRNA for factor V was present in Hep G2 cells using the same techniques. These data suggest that FV is present in human monocytes, presumably acquired by binding of plasma FV, and that the presence of this critical coagulation factor is not due to de novo synthesis.

Platelets Stimulate Thromboplastin Synthesis in Human Endothelial Cells

1983 ◽  
Vol 49 (02) ◽  
pp. 069-072 ◽  
Author(s):  
U L H Johnsen ◽  
T Lyberg ◽  
K S Galdal ◽  
H Prydz
Keyword(s):  
Endothelial Cells ◽  
De Novo ◽  
Umbilical Vein ◽  
Time Dependent ◽  
De Novo Synthesis ◽  
Human Endothelial Cells ◽  
Tumor Promotor ◽  
Coagulation Assay ◽  
Platelet Aggregates ◽  
Umbilical Vein Endothelial Cells

SummaryHuman umbilical vein endothelial cells in culture synthesize thromboplastin upon stimulation with phytohaemagglutinin (PHA) or the tumor promotor 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The thromboplastin activity is further strongly enhanced in a time dependent reaction by the presence of gel-filtered platelets or platelet aggregates. This effect was demonstrable at platelet concentrations lower than those normally found in plasma, it may thus be of pathophysiological relevance. The thromboplastin activity increased with increasing number of platelets added. Cycloheximide inhibited the increase, suggesting that de novo synthesis of the protein component of thromboplastin, apoprotein III, is necessary.When care was taken to remove monocytes no thromboplastin activity and no apoprotein HI antigen could be demonstrated in suspensions of gel-filtered platelets, platelets aggregated with thrombin or homogenized platelets when studied with a coagulation assay and an antibody neutralization technique.

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