Serum Aspartate Aminotransferase to Alanine Aminotransferase Ratio in Human and Experimental Alcoholic Liver Disease: Relationship to Histologic Changes

Enzyme ◽  
1989 ◽  
Vol 41 (2) ◽  
pp. 112-115 ◽  
Author(s):  
A. A. (a) Nanji ◽  
S. W. (a) French ◽  
C. L (b) Mendenhall
Keyword(s):  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Serum Aspartate Aminotransferase ◽  
Histologic Changes

scholarly journals Effect of Terminalia Chebula (Haritaki) on Serum Aspartate Aminotransferase, Alanine Aminotransferase in Paracetemol induced liver damage in Wister Albino Rats

2015 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Tania Yeasmin ◽  
Qazi Shamima Akhter ◽  
Syeda Tasfia Siddika ◽  
Fayeza Karim
Keyword(s):  
Body Weight ◽  
Liver Function ◽  
Liver Damage ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Basal Diet ◽  
Base Line ◽  
Terminalia Chebula ◽  
Serum Aspartate Aminotransferase ◽  
Bonferroni Test

Background: Liver plays a major role in detoxification and excretion of many endogenous and exogenous compounds. Any injury may lead to severe liver damage and impairment of liver function. Harbal plants such as Terminalia chebula (Haritaki) may have free radical scavenging activity thereby can be used for the prevention and treatment of liver damage.Objective: To observe the effect of Terminalia chebula on paracetamol induced changes of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in Wister albino rats.Methods: This experimental study was carried out in the Department of Physiology, Dhaka Medical College, Dhaka from January to December’ 2013. Total 44 rats with age 90 to 120 days, weighing between 150 to 200 gm were selected. After acclimatization for 14 days, they were divided into base line control (BC, n=11), paracetamol treated control (PC, n=11),Terminalia chebula pretreated and paracetamol treated (TCP-PCT n=11) and paracetamol pretreated and Terminalia chebula treated group (PCP-TCT, n=11). All groups received basal diet for 21 consecutive days. In addition to basal diet, rats of BC received propylene glycol (2ml/kg body weight, orally) and PC received single dose of paracetamol suspension (750mg/kg body weight, orally) on 21st day. Rats of TCP-PCT received Terminalia chebula extract (200 mg/kg body weight, orally) for 21 consecutive days and paracetamol suspension (750mg/kg body weight, orally) on 21st day. Again, rats of PCP-TCT received paracetamol suspension (750mg/kg body weight, orally) on the 1st day and Terminalia chebula extract (200 mg/kg body weight orally) for 21 consecutive days. All rats were sacrificed on 22nd day and then blood samples were collected. For assessment of liver function serum AST and ALT levels were estimated by using standard laboratory kits. The statistical analysis was done by one way ANOVA and post hoc Bonferroni test as applicable.Results: The mean serum AST and ALT levels were significantly (p<0.001) higher in PC in comparison to those of BC. Serum AST and ALT levels of all experimental groups were significantly (P<0.001) lower than PC group. Conclusion: From the results of this study, it may be concluded that Terminalia chebula may have some hepatoprotective effects in paracetamol induced liver damage in rats.Bangladesh Soc Physiol. 2015, June; 10(1): 1-5

scholarly journals CLINICAL AND LABORATORIAL CHARACTERISTICS OF THE LIVER IN AGED AND NON-AGED ALCOHOLICS

2019 ◽  
Vol 12 (3) ◽  
pp. 521
Author(s):  
Paulo Borini ◽  
Romeu Cardoso Guimarães ◽  
Zamir Calamita
Keyword(s):  
Portal Hypertension ◽  
Liver Disease ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Reference Value ◽  
Cross Sectional Study ◽  
Gamma Glutamyl Transferase ◽  
Aged Patients ◽  
Cross Sectional ◽  
Glutamyl Transferase

The medical bibliography is deficient in research involving structural and laboratory abnormalities of the liver in asymptomatic and oligosymptomatic male alcoholics. The present study describes the alterations in tests that evaluate liver aggression, lesion and dysfunction, also correlating these with the changes in the consistency and sensitivity of the liver in aged and non-aged alcoholics. Cross-sectional study involving 100 alcoholic men, 50 aged and 50 non-aged. Of the aged and non-aged, respectively, the liver was palpable in 68% and 80%, the spleen was percutable in 72% and 74% and palpable in 12% and 22%, non-painful soft hepatomegaly was observed in 14% and 8%, painful hepatomegaly in 0% and 2%, firm hepatomegaly (painful and non-painful) in 54% and 70%, hepatomegaly and splenomegaly (percutable and palpable spleen) in 84% and 84%, and portal hypertension in 10 % and 14%. Elevated levels of aspartate aminotransferase were observed in 66% and 84%, alanine aminotransferase in 24% and 60%, gamma glutamyl transferase in 46% and 82% and alkaline phosphatase in 0% and 16% of the aged and non-aged, respectively. Elevation of aspartate aminotransferase greater than twice the reference value and the ratio aspartate aminotransferase to alanine aminotransferase greater than 2 were observed in 18% and 42% and 18% and 10% of the aged and non-aged, respectively. Hypoalbuminemia and hyperbilirubinemia were observed in 70% and 24% of the aged and 40% and 44% of the non-aged, respectively. The clinical and laboratory alterations observed are compatible with acute liver disease in 18% and 42%; chronic liver disease in 54% and 70%; portal hypertension in 10% and 14% of aged and non-aged patients, respectively.

Sources of Error in Spectrophotometric Measurement of Aspartate Aminotransferase and Alanine Aminotransferase Activities in Serum

1974 ◽  
Vol 20 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Denis O Rodgerson ◽  
Iris M Osberg
Keyword(s):  
Enzyme Activity ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Short Interval ◽  
Error Sources ◽  
Double Beam ◽  
Serum Aspartate Aminotransferase ◽  
Endogenous Substrate ◽  
Coupled Reactions ◽  
Serum Glutamate

Abstract We examined the measurement of serum aspartate and alanine aminotransferase activities with a short-interval enzyme-activity analyzer. Double-beam spectrophotometry was used to elucidate the source of errors in such measurements. For both enzymes the principal error sources are: (a) the presence of endogenous substrate for lactate dehydrogenase, and (b) reaction of 2-oxoglutarate with serum glutamate dehydrogenase and ammonium ions added to the reaction mixture in admixture with the secondary enzymes of the coupled reactions. In the case of serum aspartate aminotransferase, a less important source of error is the reaction of serum alanine aminotransferase with endogenous substrate. Use of 2-oxoglutarate as a reaction initiator in conventional methods causes errors. Suitable blank reagent mixtures are described that permit accurate, rapid measurement of these activities by double-beam spectrophotometry in a short-interval enzyme-activity analyzer.

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