scholarly journals Concentration of Hematopoietic Progenitor Cells from Human Bone Marrow by a New Type of Blood Component Separator

Vox Sanguinis ◽  
1985 ◽  
Vol 49 (2) ◽  
pp. 154-160
Author(s):  
C.G. Figdor ◽  
T. de Witte ◽  
R. Raymakers ◽  
C. Haanen ◽  
W.S. Bont
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hematopoietic Progenitor Cells ◽  
Blood Component ◽  
Hematopoietic Progenitor ◽  
New Type

Use of Merocyanine 540 for the Isolation of Quiescent, Primitive Human Bone Marrow Hematopoietic Progenitor Cells

1999 ◽  
Vol 8 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Robert E. Pyatt ◽  
Laura L. Jenski ◽  
Ruth Allen ◽  
Ken Cornetta ◽  
Rafat Abonour ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Merocyanine 540

Characterization and functional analysis of laminin isoforms in human bone marrow

Blood ◽  
2000 ◽  
Vol 96 (13) ◽  
pp. 4194-4203
Author(s):  
Ulrich Siler ◽  
Martina Seiffert ◽  
Sabine Puch ◽  
Allan Richards ◽  
Beverly Torok-Storb ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Adhesive Interactions

Laminins are a family of disulfide-linked heterotrimeric proteins consisting of 3 different subunits termed α, β, and γ chains. Combinations of 11 characterized laminin subunits (α1-α5, β1-β3, and γ1-γ3) generate at least 12 laminin isoforms, which can serve different functions. Although expression of laminin in the hematopoietic microenvironment has been known for many years, the nature of the laminin isoforms present in the human bone marrow is poorly characterized. The present study attempts to clarify this issue. Reverse transcriptase–polymerase chain reaction analysis of human bone marrow stromal cells suggested the expression of many laminin isoforms in the marrow. Northern blot and immunoblot analysis, however, showed that laminin-8/9 and laminin-10/11 are the most abundant laminin isoforms synthesized by human bone marrow stromal cells. Other isoforms, if present, certainly play a minor role in the hematopoietic microenvironment. Functionally, laminin-10/11 preparations showed strong adhesive interactions with human CD34+ cell lines. Antibodies against the β1 integrin subunit inhibited these interactions. Other laminin isoforms, especially laminin-1 and laminin-2/4, showed only weak or no adhesive interactions with the hematopoietic cell lines tested, explaining former negative results. In addition to its adhesion-mediating properties, laminin-10/11 preparations also showed a mitogenic activity for human hematopoietic progenitor cells. Taken together, these data suggest that laminin in the bone marrow plays a hitherto unexpected important function in the development of hematopoietic progenitor cells.

Sign in / Sign up

Export Citation Format

Share Document