Bg^b Expression in Relation to the HLA-B17 Antigen Splits Bw57 and Bw58 and the Cross-Reactions of Anti-Bg^b Antibodies

Vox Sanguinis ◽  
1982 ◽  
Vol 43 (1) ◽  
pp. 1-10
Author(s):  
Marilyn S. Pollack ◽  
Mary N. Crawford ◽  
Harriet M. Robinson ◽  
Rachel Berger ◽  
Bernice Sabo ◽  
...  
Keyword(s):  
Cross Reactions ◽  
The Cross

scholarly journals Naturally induced auto-anit-idiotypic antibodies. Induction by identical idiotopes in some members of an outbred rabbit family.

1982 ◽  
Vol 156 (3) ◽  
pp. 860-872 ◽  
Author(s):  
S B Binion ◽  
L S Rodkey
Keyword(s):  
Germ Line ◽  
Antibody Responses ◽  
Idiotypic Network ◽  
Paternal Origin ◽  
Cross Reactions ◽  
Network Concept ◽  
Normal Individuals ◽  
Micrococcus Lysodeikticus ◽  
The Cross ◽  
Antibody Population

Naturally induced auto-anti-idiotypic (AAI) antibody responses specific for antimicrococcal antibody idiotypes were detected in 42% of the rabbits in a family immunized with Micrococcus lysodeikticus. The natural AAI response of each rabbit recognized only a portion (11-41%) of that individual's total antimicrococcal antibody population. Cross-reactions of idiotypes were observed within the group of rabbits exhibiting natural AAI responses. Examination of the basis for the cross-reactions showed that the natural AAI antisera recognized identical idiotopes on the antimicrococcal F(ab')2 fragments from each rabbit that made an AAI response. The cross-reactive idiotopes were shown to be of paternal origin and were found in the antimicrococcal antibodies of each offspring. The data strongly support the idiotypic network concept that naturally induced AAI responses may occur routinely in outbred normal individuals as a result of antigenic stimulation. Further, the data suggest that the induction of regulatory AAI antibody responses in outbred rabbits may depend on the expression of particular germ line idiotopes.

scholarly journals Sharing of Ia antigens between species. I. Detection of Ia specificities shared by rats and mice.

1977 ◽  
Vol 146 (2) ◽  
pp. 381-393 ◽  
Author(s):  
D H Sachs ◽  
G W Humphrey ◽  
J K Lunney
Keyword(s):  
Lymphoid Cells ◽  
Mouse Strains ◽  
Cell Populations ◽  
Complex Chemical ◽  
Cross Reactions ◽  
Ia Antigens ◽  
Mouse Cells ◽  
The Cross ◽  
Distribution Studies ◽  
Rats And Mice

A mouse anti-rat xenogeneic antiserum, B10.D2 anti-BN, has been found to react with a subpopulation of lymphoid cells of certain mouse strains. The corresponding alloantiserum, B10.D2 anti-B10.BR, reacted in analogous fashion with lymphoid cells of BN rats. In the case of the cross-reaction on mouse cells, mapping studies indicated that at least part of the reactivity was with the product of gene(s) determined by the I-A subregion of the H-2 complex. Chemical isolation studies with radiolabeled cell surface preparations indicated that the antigens detected in both mouse and rat had mol wt characteristic of Ia antigens (35,000 and 28,000 dalton molecules). Testing of fractionated spleen cell populations revealed that the cross-reactive antigens were expressed predominatly on B cells, but that a subpopulation of T cells were also reactive. Wider strain and species distribution studies are in progress to determine the extent of such Ia cross-reactions between species and to further assess the practical and theoretical importance of such cross-reactions.

scholarly journals Serological studies ofBacteroides fragilis

1977 ◽  
Vol 79 (2) ◽  
pp. 233-241 ◽  
Author(s):  
K. M. Elhag ◽  
K. A. Bettelheim ◽  
Soad Tabaqchali
Keyword(s):  
Bacteroides Fragilis ◽  
Gram Negative ◽  
Biochemical Characteristics ◽  
Cross Reactions ◽  
Homologous Antigen ◽  
Specific Antisera ◽  
Serological Studies ◽  
The Cross ◽  
O Antigens

SUMMARYUsing direct agglutination methods, a simple serological scheme for the classification ofBacteroides fragilisis described. Twenty strains ofB. fragiliswere selected by a process of successive screening from 151 strains obtained from various sources. O-antigens were prepared from the 20 strains, and used to raise antisera in rabbits.Each of the 20 antisera reacted with its homologous antigen and eight antisera cross-reacted with other subspecies. These cross-reactions were successfully removed after absorption of the antisera with the cross-reacting antigens, resulting in 19 type-specific antisera, titres ranging from 40 to 320, and 19 distinct serotypes ofB. fragilis. There was no correlation between the antigenic and the biochemical characteristics of these strains and no cross-reactions occurred with other gram-negative anaerobes,B. melaninogenicus, Sphaerophorus necrophorusandFuso-bacterium necrogenes.

Human Saliva and Semen: Evidence for Proteins Common to Both Which Do Not Occur in Serum

1981 ◽  
Vol 60 (2) ◽  
pp. 179-184 ◽  
Author(s):  
P. D. Eckersall ◽  
J. A. Beeley
Keyword(s):  
Antibacterial Activity ◽  
Affinity Chromatography ◽  
Human Serum ◽  
Serum Protein ◽  
Serum Proteins ◽  
Rabbit Antiserum ◽  
Human Saliva ◽  
Cross Reactions ◽  
Whole Saliva ◽  
The Cross

1. Rabbit antiserum to human whole saliva cross-reacts with both human serum and semen. After absorption of the antiserum by affinity chromatography on a column of immobilized serum protein, the cross-reactions with serum were eliminated. 2. The absorbed antiserum, however, still cross-reacted with semen thus indicating the presence of proteins with immunological similarity in both saliva and semen, but which did not occur in serum. 3. Some of these proteins clearly showed a reaction of complete immunological identity between saliva and semen. 4. The presence of the non-serum proteins in both saliva and semen might be related to common functions in both such as lubrication or antibacterial activity.

scholarly journals THE SIGNIFICANCE OF M AND T ANTIGENS IN THE CROSS REACTIONS BETWEEN CERTAIN TYPES OF GROUP A HEMOLYTIC STREPTOCOCCI

1940 ◽  
Vol 71 (4) ◽  
pp. 539-550 ◽  
Author(s):  
Rebecca C. Lancefield
Keyword(s):  
Specific Antigen ◽  
Specific Protein ◽  
T Antigens ◽  
Cross Reactions ◽  
Group A ◽  
Specific Antigens ◽  
The Cross ◽  
Type 3

In any one strain the occurrence of the previously recognized type-specific protein, M, is usually completely correlated with the presence of the recently recognized type-specific antigen, T. Strain C203 is exceptional in having the T substance of type 1 as well as the two type-specific antigens, M and T, characteristic of type 3. It does not have the M antigen of type 1. While other strains with similar antigenic peculiarities have not been encountered, it is probable that they occur, and the existence of such anomalies must be suspected when unusual serological reactions occur.

Magnetic Assistance for Suppressing Cross-Reactions Between Biomolecules in Immunoassay

2010 ◽  
Author(s):  
Chin-Yih Hong ◽  
Shieh-Yueh Yang ◽  
Herng-Er Horng ◽  
Hong-Chang Yang
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Centrifugal Force ◽  
Applied Magnetic Field ◽  
Cross Reactions ◽  
Target Molecules ◽  
The Cross ◽  
Alternative Current ◽  
The Magnetic Field ◽  
Current Magnetic Field

A method involving the use of magnetic nanoparticles to suppress the cross-reactions in immunoassay is developed. Antibodies are coated onto magnetic nanoparticles. These antibodies bind with target and non-target molecules. Once an alternative-current magnetic field is applied, magnetic nanoparticles oscillate with the magnetic field. The target and non-target molecules attached onto magnetic nanoparticles via antibodies experience a centrifugal force, which is against the association between antibodies and target/non-target molecules. Theoretically, the centrifugal force is proportional to the square of the frequency of the applied magnetic field. Thus, the strength of the centrifugal force can be manipulated by changing the frequency of the applied magnetic field. By well controlling the frequency of applied magnetic field, the centrifugal force can be stronger than the binding between antibodies and non-target molecules, but still weaker than that of target molecules. Consequently, the binding between antibodies and non-target molecules is broken by the centrifugal force.

THE DETERMINATION OF 5α-ANDROSTANE-3α,17β-DIOL IN HUMAN PLASMA BY RADIOIMMUNOASSAY

1978 ◽  
Vol 88 (4) ◽  
pp. 778-786 ◽  
Author(s):  
P. Laband ◽  
J. A. F. Tresguerres ◽  
B. P. Lisboa ◽  
U. Volkwein ◽  
J. Tamm
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Plasma ◽  
Cross Reactions ◽  
Idiopathic Hirsutism ◽  
Coefficients Of Variation ◽  
The Cross ◽  
Normal Males ◽  
Layer Chromatography

ABSTRACT Antibodies have been raised in rabbits against 3α,17β-dihydroxy-5α-androstane-6-0-carboxymethyloxime coupled with Cohn's fraction IV-4. The antiserum exhibited significant cross reactions with 5β-androstane-3α,17β-diol, 5α-dihydrotestosterone, and testosterone. No cross reactions were observed with 5α-androstane-3β,17β-diol and 5-androstene-3β,17β-diol. The methodological criteria for the measurement of 5α-androstane-3α,17β-diol in human plasma were as follows: The specificity was ensured by separating the cross reacting steroids by thin layer chromatography. The intraassay and interassay coefficients of variation were found to be 6.2 and 10.2 %, respectively. The sensitivity was 30 pg. The recovery of different amounts of 5α-androstane-3α,17β-diol added to human plasma (80, 120, and 200 pg) yielded 91.3, 92.5, and 93.5%, respectively. The following concentrations of 5α-androstane-3α,17β-diol have been determined in human plasma (mean ± sd, ng/dl): Normal males: 18.98 ± 5.9; normal females: 2.65 ± 0.27; females with idiopathic hirsutism: 11.9 ± 6.4; pre-pubertal children: not detectable.

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