Neonatal Alloimmune Thrombocytopenia in Taiwan Due to an Antibody against a Labile Component of HPA-3a (Bak^a)

Marie Lin; Shiow-Hua Shieh; Der-Cherng Liang; Ting-Fan Yang; Yoichi Shibata

doi:10.1159/000462865

Neonatal Alloimmune Thrombocytopenia in Taiwan Due to an Antibody against a Labile Component of HPA-3a (Bak^a)

Vox Sanguinis ◽

10.1159/000462865 ◽

1995 ◽

Vol 69 (4) ◽

pp. 336-340

Author(s):

Marie Lin ◽

Shiow-Hua Shieh ◽

Der-Cherng Liang ◽

Ting-Fan Yang ◽

Yoichi Shibata

Keyword(s):

Labile Component ◽

Alloimmune Thrombocytopenia

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Neonatal Alloimmune Thrombocytopenia in Taiwan Due to an Antibody against a Labile Component of HPA-3a (Bak)

Vox Sanguinis ◽

10.1111/j.1423-0410.1995.tb00369.x ◽

1995 ◽

Vol 69 (4) ◽

pp. 336-340 ◽

Cited By ~ 12

Author(s):

Marie Lin ◽

Shiow-Hua Shieh ◽

Der-Cherng Liang ◽

Ting-Fan Yang ◽

Yoichi Shibata

Keyword(s):

Labile Component ◽

Alloimmune Thrombocytopenia

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Neonatal alloimmune thrombocytopenia due to an antibody against a labile component of human platelet antigen-3b (Bakb)

Transfusion Medicine ◽

10.1111/j.1365-3148.2004.00537.x ◽

2004 ◽

Vol 14 (6) ◽

pp. 419-423 ◽

Cited By ~ 13

Author(s):

S. Kataoka ◽

H. Kobayashi ◽

K. Chiba ◽

M. Nakamura ◽

S. Shinada ◽

...

Keyword(s):

Human Platelet ◽

Platelet Antigen ◽

Human Platelet Antigen ◽

Labile Component ◽

Alloimmune Thrombocytopenia

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Neonatal alloimmune thrombocytopenia (NATP) caused by anti-BAKa (HPA-3a) maternal antibody detectable only in reactions with intact platelets

Transfusion Medicine ◽

10.1046/j.1365-3148.2000.00261-17.x ◽

2000 ◽

Vol 10 (4) ◽

pp. 329-329

Author(s):

B. R. Curtis ◽

J. G. McFarland ◽

C. R. Harrison ◽

D. D. Ebert ◽

R. H. Aster

Keyword(s):

Maternal Antibody ◽

Alloimmune Thrombocytopenia

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Localization of the Br Polymorphism on a 144 bp Exon of the GPIa Gene and Its Application in Platelet DNA Typing

Thrombosis and Haemostasis ◽

10.1055/s-0038-1642498 ◽

1994 ◽

Vol 71 (05) ◽

pp. 651-654 ◽

Cited By ~ 50

Author(s):

Rainer Kalb ◽

Sentot Santoso ◽

Katja Unkelbach ◽

Volker Kiefel ◽

Christian Mueller-Eckhardt

Keyword(s):

Genomic Organization ◽

Fragment Length Polymorphism ◽

Human Platelet ◽

Dna Typing ◽

Rflp Analysis ◽

Serological Typing ◽

Allele Specific ◽

Polymerase Chain ◽

Alloimmune Thrombocytopenia ◽

Rflp Typing

SummaryAlloimmunization against the human platelet alloantigen system Br (HPA-5) is the second most common cause of neonatal alloimmune thrombocytopenia (NAIT) in Caucasian populations. We have recently shown that a single base polymorphism at position 1648 on platelet mRNA coding for GPIa results in an aminoacid substitution at position 505 on the mature GPIa which is associated with the two serological defined Br phenotypes.Since DNA-typing of platelet alloantigens offers possibilities for useful clinical applications, we designed genomic DNA-based restriction fragment length polymorphism (RFLP) typing for Br alloantigens. To establish this technique we analyzed the genomic organization of GPIa adjacent to the polymorphic base. Using the polymerase chain reaction (PCR) of blood cell DNA we have identified two introns (approximately 1.7 and 1.9 kb) flanking a 144 bp coding sequence of the GPIa gene encompassing the polymorphic base 1648. Based on the in- tron sequence, a PCR primer was constructed to amplify a 274 bp fragment which was used for allele-specific RFLP to determine the Br genotypes. The results of RFLP analysis using Mnll endonuclease obtained from 15 donors (2 Br37*, 2 Br^ and 11 Brb/b) correlate perfectly with serological typing by monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay.

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