Hepatitis A Virus PCR Analysis of Clotting Factor Concentrates and Elimination of Non-Enveloped Viruses in Their Manufacture

Vox Sanguinis ◽  
1994 ◽  
Vol 67 (1) ◽  
pp. 78-79
Author(s):  
H. Mohr ◽  
B. Lambrecht ◽  
F. Marks ◽  
A. Tschech
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Clotting Factor ◽  
Pcr Analysis ◽  
Enveloped Viruses ◽  
Clotting Factor Concentrates

Hepatitis A Virus Sequence Detected in Clotting Factor Concentrates Associated With Disease Transmission

Biologicals ◽  
1998 ◽  
Vol 26 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Betty H. Robertson ◽  
Miriam J. Alter ◽  
Beth P. Bell ◽  
Bruce Evatt ◽  
Karen A. McCaustland ◽  
...  
Keyword(s):  
Disease Transmission ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Clotting Factor ◽  
Virus Sequence ◽  
Clotting Factor Concentrates

“In vitro” and in Animal Model Studies on a Double Virus- Inactivated Factor VIII Concentrate

1995 ◽  
Vol 74 (03) ◽  
pp. 868-873 ◽  
Author(s):  
Silvana Arrighi ◽  
Roberta Rossi ◽  
Maria Giuseppina Borri ◽  
Vladimir Lesnikov ◽  
Marina Lesnikov ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Animal Model ◽  
Factor Viii ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Inactivation ◽  
Enveloped Viruses ◽  
Model Studies ◽  
Heat Treated

SummaryTo improve the safety of plasma derived factor VIII (FVIII) concentrate, we introduced a final super heat treatment (100° C for 30 min) as additional virus inactivation step applied to a lyophilized, highly purified FVIII concentrate (100 IU/mg of proteins) already virus inactivated using the solvent/detergent (SID) method during the manufacturing process.The efficiency of the super heat treatment was demonstrated in inactivating two non-lipid enveloped viruses (Hepatitis A virus and Poliovirus 1). The loss of FVIII procoagulant activity during the super heat treatment was of about 15%, estimated both by clotting and chromogenic assays. No substantial changes were observed in physical, biochemical and immunological characteristics of the heat treated FVIII concentrate in comparison with those of the FVIII before heat treatment.

Hepatitis A virus infections associated with clotting factor concentrate in the United States

Transfusion ◽  
1998 ◽  
Vol 38 (6) ◽  
pp. 573-579 ◽  
Author(s):  
JM Soucie ◽  
BH Robertson ◽  
BP Bell ◽  
KA McCaustland ◽  
BL Evatt
Keyword(s):  
United States ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
The United States ◽  
Clotting Factor ◽  
Virus Infections ◽  
Factor Concentrate

A Polymerase Chain Reaction–Based Method for the Detection of Hepatitis A Virus in Produce and Shellfish

2002 ◽  
Vol 65 (2) ◽  
pp. 393-402 ◽  
Author(s):  
BISWENDU B. GOSWAMI ◽  
MICHAEL KULKA ◽  
DIANA NGO ◽  
PHILLIP ISTAFANOS ◽  
THOMAS A. CEBULA
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
General Procedure ◽  
Pcr Analysis ◽  
Genomic Rna ◽  
Chain Reaction ◽  
Viral Origin ◽  
Polymerase Chain ◽  
Test Virus

Outbreaks of gastroenteritis that are suspected to be of viral origin are on the rise. Thus, there is a need for regulatory agencies entrusted with food safety to develop adequate techniques for the detection of viruses in foods. We have established a general procedure for the detection of hepatitis A virus (HAV) in shellfish that, with minor modifications, is also applicable to fresh produce such as cilantro. Total RNA was isolated from shellfish or cilantro, followed by isolation of poly(A)-containing RNA. Because HAV genomic RNA contains a poly(A) tail, the isolation of poly(A)-containing RNA also enriches HAV genomic RNA. Reverse transcription was used to convert the RNA to cDNA, and then amplification was carried out by polymerase chain reaction (PCR). Reamplification with internal primers was used to improve the quality and the quantity of amplified DNA, allowing for post-PCR analysis such as sequence identification of the viral strain. With this procedure, multiple samples could be analyzed in four working days by a single trained individual. The nominal sensitivity of detection of the procedure was 0.15 TCID50 (50% tissue culture infective dose) per 0.62 g of tissue with a test virus. The direct RNA isolation protocol avoided pitfalls associated with whole-virus purification procedures by replacing virus precipitation steps involving polyethylene glycol and Procipitate with phenol extraction. The method is straightforward and reliable. We successfully used this procedure to detect naturally occurring HAV in clams involved in a gastroenteritis outbreak, as well as in cilantro artificially contaminated with a test virus.

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