Studies in Red Blood Cell Preservation. 8. Liquid Storage of Red Cells in a Glycerol- Containing Additive Solution

Vox Sanguinis ◽  
1994 ◽  
Vol 67 (2) ◽  
pp. 139-143
Author(s):  
U.J. Dumaswala ◽  
N.L. Bentley ◽  
T.J. Greenwalt
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Red Cells ◽  
Liquid Storage ◽  
Additive Solution ◽  
Cell Preservation

Studies in Red Blood Cell Preservation 3. A Phosphate-Ammonium-Adenine Additive Solution

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 94-99 ◽  
Author(s):  
T.J. Greenwalt ◽  
C.G. McGuinness ◽  
U.J. Dumaswala ◽  
H.W. Carter
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Additive Solution ◽  
Cell Preservation

Studies in Red Blood Cell Preservation - 3. A Phosphate-Ammonium-Adenine Additive Solution

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 94-99
Author(s):  
T.J. Greenwalt ◽  
C.G. McGuinness ◽  
U.J. Dumaswala ◽  
H.W. Carter
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Additive Solution ◽  
Cell Preservation

scholarly journals Red Blood-Cell Antigens in Some Lower Vertebrates

1956 ◽  
Vol 33 (1) ◽  
pp. 249-255
Author(s):  
DOREEN E. ASHHURST
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Groups ◽  
Red Cells ◽  
Lower Vertebrates ◽  
Tree Frogs

1. It was not possible to find evidence for blood groups in frogs (one species) or in fish (three species). 2. Frogs could not be induced to make antibodies after injection with red cells from another frog. 3. Frogs, toads and tree frogs have a B antigen; newts probably have not.

scholarly journals Increased Ca++, Mg++, and Na+ + K+ ATPase activities in erythrocytes of sickle cell anemia

Blood ◽  
1982 ◽  
Vol 60 (6) ◽  
pp. 1332-1336 ◽  
Author(s):  
MG Luthra ◽  
DA Sears
Keyword(s):  
Enzyme Activity ◽  
Blood Cell ◽  
Atpase Activity ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Blood Cell ◽  
Calcium Binding ◽  
Red Cells ◽  
Low Density ◽  
Red Cell

Abstract To determine whether diminished activity of the Ca++ extrusion pump could account for the high levels of red blood cell (RBC) Ca++ in sickle cell anemia (SS), we measured calmodulin-sensitive Ca++ ATPase activity in normal and SS RBC. Hemolysates prepared with saponin were compared, since such preparations expressed maximum ATPase activities, exceeding isolated membranes or reconstituted systems of membranes plus cytosol, SS RBC hemolysates had greater Ca++ ATPase activity than normal hemolysates; they exhibited higher Mg++ and Na+ + K+ ATPase activities as well. Assays on density (age) fractions of SS and normal red cells demonstrated that all ATPase activities were highest in low density (young) cells, and activities in SS red cells exceeded those in normals in all fractions studied. Thus, when studied under conditions that maximize enzyme activity, Ca++ ATPase activity, like Mg++ and Na+ + K+ ATPase, is actually increased in SS RBC, probably due to the young red cell population present. The elevated Ca++ levels in these cells are more likely due to an increased Ca++ leak or abnormal calcium binding than to defective extrusion by the ATPase pump.

scholarly journals RED CELL STROMA PROTEIN RICH IN VITAMIN B12 DURING ACTIVE REGENERATION

1955 ◽  
Vol 102 (6) ◽  
pp. 725-731 ◽  
Author(s):  
G. H. Whipple ◽  
F. S. Robscheit-Robbins ◽  
W. F. Bale
Keyword(s):  
Blood Cell ◽  
Vitamin B12 ◽  
Pernicious Anemia ◽  
Red Blood Cell ◽  
Red Cells ◽  
Cell Regeneration ◽  
Red Cell ◽  
Low Levels ◽  
Active Regeneration ◽  
Protein Rich

During active blood regeneration in anemia in dogs an increase occurs in the stroma protein of the red cells. When vitamin B12 with radioactive cobalt is given at the start of this blood regeneration one finds concentration of labeled B12 in the stroma protein but not in the hemoglobin. After the acute phase of red cell regeneration is ended the concentration of B12 in stroma protein falls rapidly to very low levels within 2 weeks. Subsequent episodes of red blood cell regeneration seems not to cause remobilization of radioactive cobalt into red cells from other body stores. It appears that the vitamin B12 is a factor of importance in the first steps of stroma protein formation in the first few days of the life of the red cell in the dog. This response in dogs and the response in pernicious anemia to vitamin B12 may have some points in common. Distribution of the B12-radioactive cobalt in the organs and tissues at autopsy has been recorded. Some very suggestive localizations were noted and some variation 1 week and 7 weeks after B12 injections. Radioactive cobalt escapes in the urine during the weeks following B12 injections.

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