Prospective Evaluation of the Chemiluminescence Test for the Detection of Granulocyte Antibodies: Comparison with the Granulocyte Immunofluorescence Test

Vox Sanguinis ◽  
1994 ◽  
Vol 66 (2) ◽  
pp. 141-147 ◽  
Author(s):  
G.F. Lucas
Keyword(s):  
Prospective Evaluation ◽  
Immunofluorescence Test ◽  
Granulocyte Antibodies

IMMUNOHEMATOLOGY: Rapid screening of granulocyte antibodies with a novel assay: flow cytometric granulocyte immunofluorescence test

Transfusion ◽  
2009 ◽  
Vol 49 (12) ◽  
pp. 2700-2708 ◽  
Author(s):  
Xuan Duc Nguyen ◽  
Brigitte Flesch ◽  
Ulrich J. Sachs ◽  
Hartmut Kroll ◽  
Harald Klüter ◽  
...  
Keyword(s):  
Rapid Screening ◽  
Immunofluorescence Test ◽  
Flow Cytometric ◽  
Granulocyte Antibodies

scholarly journals A Novel Tool for High-Throughput Screening of Granulocyte-Specific Antibodies Using the Automated Flow Cytometric Granulocyte Immunofluorescence Test (Flow-GIFT)

2011 ◽  
Vol 11 ◽  
pp. 302-309 ◽  
Author(s):  
Xuan Duc Nguyen ◽  
Thomas Dengler ◽  
Monika Schulz-Linkholt ◽  
Harald Klüter
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Blood Donors ◽  
Rapid Screening ◽  
Antibody Detection ◽  
Immunofluorescence Test ◽  
Flow Cytometric ◽  
Reference Methods ◽  
Test Flow ◽  
Granulocyte Antibodies

Transfusion-related acute lung injury (TRALI) is a severe complication related with blood transfusion. TRALI has usually been associated with antibodies against leukocytes. The flow cytometric granulocyte immunofluorescence test (Flow-GIFT) has been introduced for routine use when investigating patients and healthy blood donors. Here we describe a novel tool in the automation of the Flow-GIFT that enables a rapid screening of blood donations. We analyzed 440 sera from healthy female blood donors for the presence of granulocyte antibodies. As positive controls, 12 sera with known antibodies against anti-HNA-1a, -b, -2a; and -3a were additionally investigated. Whole-blood samples from HNA-typed donors were collected and the test cells isolated using cell sedimentation in a Ficoll density gradient. Subsequently, leukocytes were incubated with the respective serum and binding of antibodies was detected using FITC-conjugated antihuman antibody. 7-AAD was used to exclude dead cells. Pipetting steps were automated using the Biomek NXp Multichannel Automation Workstation. All samples were prepared in the 96-deep well plates and analyzed by flow cytometry. The standard granulocyte immunofluorescence test (GIFT) and granulocyte agglutination test (GAT) were also performed as reference methods. Sixteen sera were positive in the automated Flow-GIFT, while five of these sera were negative in the standard GIFT (anti—HNA 3a, n = 3; anti—HNA-1b, n = 1) and GAT (anti—HNA-2a, n = 1). The automated Flow-GIFT was able to detect all granulocyte antibodies, which could be only detected in GIFT in combination with GAT. In serial dilution tests, the automated Flow-GIFT detected the antibodies at higher dilutions than the reference methods GIFT and GAT. The Flow-GIFT proved to be feasible for automation. This novel high-throughput system allows an effective antigranulocyte antibody detection in a large donor population in order to prevent TRALI due to transfusion of blood products.

Frozen-stored granulocytes can be used for an immunofluorescence test to detect granulocyte antibodies

Transfusion ◽  
2001 ◽  
Vol 41 (10) ◽  
pp. 1268-1272 ◽  
Author(s):  
Motoko Nishimura ◽  
Shigeki Mitsunaga ◽  
Takeo Juji
Keyword(s):  
Immunofluorescence Test ◽  
Granulocyte Antibodies
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