Human Monoclonal Antibodies against the Rhesus D Antigen from Women with Severe Rh Immunization Submitted to High-Dose Intravenous Immunoglobulin Treatment

Vox Sanguinis ◽  
1994 ◽  
Vol 66 (1) ◽  
pp. 55-60 ◽  
Author(s):  
C. del Aguila ◽  
M.V. Guillén ◽  
C. de la Cámara ◽  
R. Llopis
Keyword(s):  
Monoclonal Antibodies ◽  
Intravenous Immunoglobulin ◽  
High Dose ◽  
Human Monoclonal Antibodies ◽  
Immunoglobulin Treatment ◽  
D Antigen ◽  
Rh Immunization

High-dose intravenous immunoglobulin treatment in cryptogenic West and Lennox-Gastaut syndrome; an add-on study

1994 ◽  
Vol 153 (10) ◽  
pp. 762-769 ◽  
Author(s):  
B. G. M. van Engelen ◽  
W. O. Renier ◽  
C. M. R. Weemaes ◽  
P. F. W. Strengers ◽  
P. J. H. Bernsen ◽  
...  
Keyword(s):  
Intravenous Immunoglobulin ◽  
High Dose ◽  
Immunoglobulin Treatment

scholarly journals High-Dose Intravenous Immunoglobulin Treatment Activates Complement In Vivo

1998 ◽  
Vol 48 (3) ◽  
pp. 312-317 ◽  
Author(s):  
Mollnes ◽  
Hogasen ◽  
De Carolis ◽  
Vaquero ◽  
Nielsen ◽  
...  
Keyword(s):  
Intravenous Immunoglobulin ◽  
High Dose ◽  
Immunoglobulin Treatment

scholarly journals Structural Basis of Human Parechovirus Neutralization by Human Monoclonal Antibodies

2015 ◽  
Vol 89 (18) ◽  
pp. 9571-9580 ◽  
Author(s):  
Shabih Shakeel ◽  
Brenda M. Westerhuis ◽  
Ari Ora ◽  
Gerrit Koen ◽  
Arjen Q. Bakker ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Neonatal Sepsis ◽  
Electron Cryomicroscopy ◽  
Human Monoclonal Antibodies ◽  
Life Threatening ◽  
Immunoglobulin Treatment

ABSTRACTSince it was first recognized in 2004 that human parechoviruses (HPeV) are a significant cause of central nervous system and neonatal sepsis, their clinical importance, primarily in children, has started to emerge. Intravenous immunoglobulin treatment is the only treatment available in such life-threatening cases and has given moderate success. Direct inhibition of parechovirus infection using monoclonal antibodies is a potential treatment. We have developed two neutralizing monoclonal antibodies against HPeV1 and HPeV2, namely, AM18 and AM28, which also cross-neutralize other viruses. Here, we present the mapping of their epitopes using peptide scanning, surface plasmon resonance, fluorescence-based thermal shift assays, electron cryomicroscopy, and image reconstruction. We determined by peptide scanning and surface plasmon resonance that AM18 recognizes a linear epitope motif including the arginine-glycine-aspartic acid on the C terminus of capsid protein VP1. This epitope is normally used by the virus to attach to host cell surface integrins during entry and is found in 3 other viruses that AM18 neutralizes. Therefore, AM18 is likely to cause virus neutralization by aggregation and by blocking integrin binding to the capsid. Further, we show by electron cryomicroscopy, three-dimensional reconstruction, and pseudoatomic model fitting that ordered RNA interacts with HPeV1 VP1 and VP3. AM28 recognizes quaternary epitopes on the capsid composed of VP0 and VP3 loops from neighboring pentamers, thereby increasing the RNA accessibility temperature for the virus-AM28 complex compared to the virus alone. Thus, inhibition of RNA uncoating probably contributes to neutralization by AM28.IMPORTANCEHuman parechoviruses can cause mild infections to severe diseases in young children, such as neonatal sepsis, encephalitis, and cardiomyopathy. Intravenous immunoglobulin treatment is the only treatment available in such life-threatening cases. In order to develop more targeted treatment, we have searched for human monoclonal antibodies that would neutralize human parechoviruses 1 and 2, associated with mild infections such as gastroenteritis and severe infections of the central nervous system, and thus allow safe treatment. In the current study, we show how two such promising antibodies interact with the virus, modeling the atomic interactions between the virus and the antibody to propose how neutralization occurs. Both antibodies can cause aggregation; in addition, one antibody interferes with the virus recognizing its target cell, while the other, recognizing only the whole virus, inhibits the genome uncoating and replication in the cell.

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