Confirmation of Hepatitis C Virus Positive Blood Donors by Immunobiotting and Polymerase Chain Reaction

Vox Sanguinis ◽  
1992 ◽  
Vol 63 (3) ◽  
pp. 198-203 ◽  
Author(s):  
H. De Beenhouwer ◽  
H. Verhaert ◽  
H. Claeys ◽  
C. Vermylen
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Blood Donors ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Introduction of NAT-testing for infections for screening blood donors in Republic of Kazakhstan

2015 ◽  
Vol 96 (3) ◽  
pp. 414-417
Author(s):  
T N Savchuk ◽  
Z K Burkitbaev ◽  
S A Abdrakhmanova ◽  
S V Skorikova ◽  
N S Kuz’min
Keyword(s):  
Human Immunodeficiency Virus ◽  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Blood Donors ◽  
Chain Reaction ◽  
B Virus ◽  
Immunodeficiency Virus ◽  
Polymerase Chain

Aim. To evaluate the effectiveness of NAT-screening (nucleic acid amplification technologies) for infections in blood donors in Kazakhstan. Methods. Statistical data of blood donors screening examinations in the Republic of Kazakhstan in 2012-2014 were evaluated. Results. In 2014, the number of examined donors increased by 3.4% compared with 2012. The number of deferrals due to positive screening results for serological markers decreased by 10.9%, while the share of such donors decreased by 13.8% [p <0.01; odds ratio (OR) - 0.86, 95% confidence interval (CI 95%) - 0.83-0.88); χ2=136.76]. In 2014, 100% of donations were screened using NAT-testing (312,510 donors). Most of the NAT-screening in Kazakhstan is performed using closed automated systems. In 2012, 1 Blood Center conducted a polymerase chain reaction screening by open circuit polymerase chain reaction systems, in 5 blood centers polymerase chain reaction was performed with manual sample preparation. In 2014, the number of deferrals due to positive NAT-testing results has increased by 44.3%, the share of such donors - by 38.7% (p <0.01; OR=1.39, 95% CI=1.15-1.67); χ2=11.82). Seronegative NAT-positive samples were discovered according to the results of discriminant test, including human immunodeficiency virus - 2 (0.8%) samples, hepatitis B virus -182 (73.4%), hepatitis C virus - 60 (24.2%), negative result - 4 (1.6%). Conclusion. The introduction of screening NAT-testing of donated blood prevented transfusion of blood infected with: human immunodeficiency virus - 1 in 150,000 donations, hepatitis B virus - 1 in 1.650 donations, hepatitis C virus - 1 in 5,000 donations.

Hepatitis C Virus Genotypes among Blood Donors and Their Recipients in Iceland Determined by the Polymerase Chain Reaction

Vox Sanguinis ◽  
1995 ◽  
Vol 69 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Arthur Löve ◽  
Agnes Smáradóttir ◽  
Sigurdur B. Thorsteinsson ◽  
Barbara Stanzeit ◽  
Anders Widell
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Blood Donors ◽  
Chain Reaction ◽  
Virus Genotypes ◽  
Hepatitis C Virus Genotypes ◽  
Polymerase Chain

Hepatitis C Virus Genotypes among Blood Donors and Their Recipients in Iceland Determined by the Polymerase Chain Reaction

Vox Sanguinis ◽  
1995 ◽  
Vol 69 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Arthur Löve ◽  
Agnes Smáradóttir ◽  
Sigurdur B. Thorsteinsson ◽  
Barbara Stanzeit ◽  
Anders Widell
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Blood Donors ◽  
Chain Reaction ◽  
Virus Genotypes ◽  
Hepatitis C Virus Genotypes ◽  
Polymerase Chain

scholarly journals Typing hepatitis C virus by polymerase chain reaction in blood donors.

1993 ◽  
Vol 39 (4) ◽  
pp. 745-749
Author(s):  
Rika Atobe ◽  
Naofumi Ito ◽  
Noriko Suzuki ◽  
Koh-ichi Ishikawa
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Blood Donors ◽  
Chain Reaction ◽  
Polymerase Chain
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