Bovine Albumin-Like Protein in Commercial Human Albumin for Clinical Use

Vox Sanguinis ◽  
1990 ◽  
Vol 59 (1) ◽  
pp. 1-5
Author(s):  
T. Murozuka ◽  
M. Moriwaka ◽  
H. Ito ◽  
S. Sekiguchi ◽  
M. Naiki ◽  
...  
Keyword(s):  
Human Albumin ◽  
Clinical Use ◽  
Bovine Albumin

Bovine Albumin-Like Protein in Commercial Human Albumin for Clinical Use

Vox Sanguinis ◽  
1990 ◽  
Vol 59 (1) ◽  
pp. 1-5 ◽  
Author(s):  
T. Murozuka ◽  
M. Moriwaka ◽  
H. Ito ◽  
S. Sekiguchi ◽  
M. Naiki ◽  
...  
Keyword(s):  
Human Albumin ◽  
Clinical Use ◽  
Bovine Albumin

STUDIES ON BACTERIAL AGGLUTINATION BY USE OF THE ANTIGLOBULIN (COOMBS) TECHNIQUE

1956 ◽  
Vol 2 (7) ◽  
pp. 657-664 ◽  
Author(s):  
Arthur C. Ford ◽  
Ralph J. DeFalco
Keyword(s):  
Antibody Titer ◽  
Human Albumin ◽  
Bacterial Cells ◽  
Specific Test ◽  
Bovine Albumin ◽  
Cell Antigen ◽  
Antiglobulin Test ◽  
Bacterial Agglutination

The Coombs antiglobulin test has been successfully applied to bacterial cells in agglutination reactions. The test is a test for attached antibody globulin. Titers obtained by this method are shown to be from one to three tubes higher than those obtained by routine agglutination tests. Polyvinylpyrrolidone (PVP), bovine albumin, and human albumin have also been shown to hasten and strengthen bacterial agglutinations. The incomplete albumin reacting antibody titer of antisera seems to be higher than that of the saline reacting, or complete antibodies. The Coombs reaction is a specific test for antibody globulin attached to sensitized cells. There is no reaction where there is no combination between antibody globulin and the cell antigen, as shown by heterologous tests.

Commercial human albumin preparations for clinical use are immunosuppressive in vitro

2006 ◽  
Vol 34 (6) ◽  
pp. 1707-1712 ◽  
Author(s):  
David Bar-Or ◽  
Gregory W. Thomas ◽  
Raphael Bar-Or ◽  
Leonard T. Rael ◽  
Kristin Scarborough ◽  
...  
Keyword(s):  
Human Albumin ◽  
Clinical Use

scholarly journals Establishing a hospital transfusion management system promotes appropriate clinical use of human albumin in Japan: a nationwide retrospective study

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yoshiteru Yano ◽  
Nobuo Sakata ◽  
Kiyohide Fushimi
Keyword(s):  
Burn Injury ◽  
Temporal Trends ◽  
Human Albumin ◽  
Care Quality ◽  
Fiscal Year ◽  
Multivariable Logistic Regression Analysis ◽  
Clinical Use ◽  
Blood Products ◽  
Albumin Administration ◽  
Human Albumin Administration

Abstract Background Despite international recommendations to establish hospital transfusion management systems to promote appropriate use of blood products, the general efficacy of establishing such systems has not been proven. This study aimed to validate the effect of establishing such systems for promoting the appropriate use of human albumin. Methods In this retrospective observational study, we used a Japanese Diagnosis Procedure Combination (DPC) database from fiscal year 2012 to 2016, which included inpatient records from approximately 1200 hospitals for payment processes in the national medical insurance system. From this existing database, containing approximately 8 million inpatient records per year, we selected patients with emergency due to “bleeding,” “sepsis,” and “burn injury,” by using the International Classification of Diseases and Injuries 10th revision (ICD-10) codes, and hospitals that had one or more patients for each disease group in each fiscal year. We conducted multivariable logistic regression analysis to estimate the relationship between human albumin administration and the state of the hospital transfusion management system. We evaluated temporal trends of mortality rate and length of stay as an indicator of care quality. Results Overall, 139,853 eligible patients admitted to 682 hospitals were selected. The results of the multivariable logistic regression analysis show that patients who were admitted to hospitals with an established hospital transfusion department introducing good practice criteria of blood products were less likely to be administered human albumin compared with those who were admitted to hospitals not introducing it, by approximately 30% for each of the three disease groups; adjusted odds ratios (95% confidential intervals) were 0.70 (0.59–0.83), 0.75 (0.69–0.81), and 0.71 (0.58–0.87) in the “bleeding,” “sepsis,” and “burn injury” groups, respectively. The temporal trends evaluation shows that there were no increasing trends of mortality rate and average length of stay against decreasing trends of human albumin administration in any disease groups. Conclusions Establishing a hospital transfusion department responsible for promoting appropriate clinical use of blood products could reduce human albumin administration for critically ill patients without loss of care quality. These findings provide support for policy makers and hospital managers to consider establishing such systems.

scholarly journals Humanized Culture Medium for Clinical Expansion of Human Erythroblasts

2010 ◽  
Vol 19 (4) ◽  
pp. 453-469 ◽  
Author(s):  
Giovanni Migliaccio ◽  
Massimo Sanchez ◽  
Francesca Masiello ◽  
Valentina Tirelli ◽  
Lilian Varricchio ◽  
...  
Keyword(s):  
Growth Factors ◽  
Cord Blood ◽  
Stem Cell Factor ◽  
Culture Medium ◽  
Mononuclear Cells ◽  
Culture Media ◽  
Ex Vivo ◽  
Fold Increase ◽  
Human Albumin ◽  
Clinical Use

Ex vivo-generated erythroblasts represent alternative transfusion products. However, inclusion of bovine components in media used for their growth precludes clinical use, highlighting the importance of developing culture media based on pharmaceutical grade reagents. In addition, because adult blood generates ex vivo lower numbers of erythroblasts than cord blood, cord blood has been proposed as the source of choice for ex vivo erythroblast production. To clarify the potential of adult blood to generate erythroblasts ex vivo, experiments were designed to identify growth factors [stem cell factor (SCF), interleukin-3 (IL-3), erythropoietin (EPO), and/or thrombopoietin (TPO)] and the optimal concentration and addition schedule of hormones (dexamethasone and estradiol) sustaining maximal erythroid amplification from adult blood mononuclear cells (MNC) using media with serum previously defined as human erythroid massive amplification culture (HEMAser). Adult MNC stimulated with SCF and IL-3 in combination with EPO generated a 6–12-fold increase in erythroid cells while TPO was ineffective. Dexamethasone and estradiol (both at 10−6 M) exerted partially overlapping but nonredundant functions. Dexamethasone was indispensable in the first 10 days of culture while estradiol was required from day 10 on. The growth factor and hormone combinations identified in HEMAser were then used to formulate a media composed of dialyzed pharmaceutical grade human albumin, human albumin-lipid liposomes, and iron-saturated recombinant human tranferrin (HEMAdef). HEMAdef sustained erythroid amplification as efficiently as HEMAser for cord blood MNC and 10-fold higher than HEMAser for adult blood MNC. In fact, the numbers of erythroblasts generated in HEMAdef by adult MNC were similar to those generated by cord blood MNC. In conclusion, this study identifies growth factors, hormone combinations, and human protein-based media that allow similar levels of ex vivo erythroid expansion from adult and cord blood MNC, paving the way to evaluate adult blood as a source of ex vivo-expanded erythroblasts for transfusion.

Heterogeneity and oxidation status of commercial human albumin preparations in clinical use*

2005 ◽  
Vol 33 (7) ◽  
pp. 1638-1641 ◽  
Author(s):  
David Bar-Or ◽  
Raphael Bar-Or ◽  
Leonard T. Rael ◽  
David K. Gardner ◽  
Denetta S. Slone ◽  
...  
Keyword(s):  
Human Albumin ◽  
Clinical Use ◽  
Oxidation Status

THE pH DEPENDENCE OF THE ADSORPTION ISOTHERM AND ABSORPTION SPECTRUM OF METHYL ORANGE BOUND TO HUMAN AND BOVINE SERUM ALBUMIN

1953 ◽  
Vol 31 (8) ◽  
pp. 734-745 ◽  
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Binding Sites ◽  
Binding Capacity ◽  
Ph Dependence ◽  
Human Albumin ◽  
Electrostatic Repulsion ◽  
Bovine Albumin ◽  
Appreciable Increase ◽  
Protein Molecules ◽  
The Difference

The difference between adsorption isotherms and absorption spectra of methyl orange bound to human and to bovine albumin at pH 6.8, 9.1, and 11.0 has been studied. Exaltation of the spectrum of methyl orange bound to human albumin is not necessarily correlated with total binding capacity. However, above pH 6.8, heterogeneity of the binding sites for methyl orange on human albumin increases so markedly that it is reflected in an appreciable increase in extinction coefficient for the first three or four anions bound. The exaltation is accompanied by an increased −ΔF° of binding. Increase in ionic strength diminishes exaltation, and denaturation of the protein destroys it. These effects were not observed for bovine albumin. Results are interpreted in terms of a limited reversible expansion of the human protein molecules, without unfolding, due to intramolecular, electrostatic repulsion between groups.

scholarly journals Quantitation of Oxidative Modifications of Commercial Human Albumin for Clinical Use

2016 ◽  
Vol 39 (3) ◽  
pp. 401-408 ◽  
Author(s):  
Teppei Takahashi ◽  
Tomoyoshi Terada ◽  
Hajime Arikawa ◽  
Kazuha Kizaki ◽  
Hiroyuki Terawaki ◽  
...  
Keyword(s):  
Human Albumin ◽  
Clinical Use ◽  
Oxidative Modifications
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