Red Cell Enzyme Activities and Properties of Mutant Pyruvate Kinase after Long-Term Storage of Red Cells in Liquid Nitrogen

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (6) ◽  
pp. 362-366
Author(s):  
S. Miwa ◽  
H. Fujii ◽  
Y. Ishida ◽  
E. Oda ◽  
T. Nakatsuji ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Pyruvate Kinase ◽  
Enzyme Activities ◽  
Red Cells ◽  
Red Cell ◽  
Long Term Storage ◽  
Cell Enzyme ◽  
Term Storage

Red Cell Enzyme Activities and Properties of Mutant Pyruvate Kinase after Long-Term Storage of Red Cells in Liquid Nitrogen

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (6) ◽  
pp. 362-366 ◽  
Author(s):  
H. Fujii ◽  
S. Miwa ◽  
Y. Ishida ◽  
E. Oda ◽  
T. Nakatsuji ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Pyruvate Kinase ◽  
Enzyme Activities ◽  
Red Cells ◽  
Red Cell ◽  
Long Term Storage ◽  
Cell Enzyme ◽  
Term Storage

Somatic polyembryogenesis in embryogenic cell masses of Piceaabies (Norway spruce) and Pinustaeda (loblolly pine) after thawing from liquid nitrogen

1987 ◽  
Vol 17 (9) ◽  
pp. 1130-1134 ◽  
Author(s):  
P. K. Gupta ◽  
D. J. Durzan ◽  
B.J. Finkle
Keyword(s):  
Liquid Nitrogen ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Cell Mass ◽  
Embryogenic Cell ◽  
Long Term Storage ◽  
Embryogenic Cell Lines ◽  
Improvement Programs ◽  
Term Storage

We describe a method for the possible cryopreservation of embryogenic callus of Piceaabies and Pinustaeda at −196 °C and the regeneration of somatic embryos from thawed cells of subcultured embryonal–suspensor masses. Piceaabies and Pinustaeda were frozen without cryoprotective agent, in the presence of dimethyl sulfoxide (10%), or in a mixture of polyethylene glycol, glucose, and dimethylsulfoxide (10, 8, and 10% w/v, respectively). Cell masses placed in plastic vials or aluminum envelopes were frozen at 1 °C/min to −30 °C and then immersed for 10 min in liquid nitrogen. Cells were thawed rapidly and placed on modified MS subculture medium. Six to seven somatic embryos per gram of fresh weight were regenerated from each piece of frozen cell mass as compared with 12–13 embryos per gram from unfrozen cells. Post-thaw cell growth was inhibited initially by up to 5 weeks. Inhibition was reversed after the third 10-day subculture. Results suggest that the long-term storage of embryogenic cell lines in liquid nitrogen may be feasible for tree improvement programs in circumstances where testing of progeny may take several years.

Long-Term Storage of Pink Lady Apples Modifies Volatile-Involved Enzyme Activities: Consequences on Production of Volatile Esters

2008 ◽  
Vol 56 (19) ◽  
pp. 9166-9174 ◽  
Author(s):  
Carmen Villatoro ◽  
Gemma Echeverría ◽  
Jordi Graell ◽  
María Luisa López ◽  
Isabel Lara
Keyword(s):  
Enzyme Activities ◽  
Long Term Storage ◽  
Term Storage ◽  
Volatile Esters

scholarly journals Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

2021 ◽  
Author(s):  
Felizitas Bajerski ◽  
Manuela Nagel ◽  
Joerg Overmann
Keyword(s):  
Risk Assessment ◽  
Quality Management ◽  
Liquid Nitrogen ◽  
Potential Risk ◽  
Biological Materials ◽  
Cross Contamination ◽  
Storage Tanks ◽  
Long Term Storage ◽  
Term Storage

Abstract Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves, the LN, the human microbiome, and the surrounding environment are possible routes of contamination and can cause cross contaminations via the LN phase. In general, the results showed that LN is typically not the source of major contaminations and only a few studies provided evidence for a risk of microbial cross contamination. So far, culture-based and culture-independent techniques detected only low amounts of microbial cells, indicating that cross contamination may occur at a very low frequency. To further minimise the potential risk of microbial cross contaminations, we recommend reducing the formation of ice crystals in cryotanks that can entrap environmental microorganisms and using sealed or second sample packing. A short survey demonstrated the awareness for microbial contaminations of storage containers among different culture collections. Although most participants consider the risk of cross contaminations in LN storage tanks as low, they prevent potential contaminations by using sealed devices and − 150 °C freezers. It is concluded that the overall risk for cross contaminations in biobanks is relatively low when following standard operating procedures (SOPs). We evaluated the potential sources in detail and summarised our results in a risk assessment spreadsheet which can be used for the quality management of biobanks. Key points • Identification of potential contaminants and their sources in LN storage tanks. • Recommendations to reduce this risk of LN storage tank contamination. • Development of a risk assessment spreadsheet to support quality management.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

scholarly journals Cryopreservation of Shoot Tips and Pollen of Potato

2017 ◽  
Vol 76 (1) ◽  
pp. 75-80
Author(s):  
Paulina Smyda-Dajmund
Keyword(s):  
Liquid Nitrogen ◽  
Shoot Tips ◽  
Popular Method ◽  
Long Term Storage ◽  
Term Storage ◽  
Direct Immersion

Abstract Cryopreservation is a frequently used method of long-term storage of potato meristems and pollen in liquid nitrogen (LN) in temperature of -196°C. This technique allows for theoretically unlimited storage of potato material. The most popular method of potato shoot tips preservation is cryopreservation by the solidification of liquids without crystallization (vitrification).The best method of pollen conservation is its direct immersion in LN. The successful regeneration after vitrification is genotype-dependent, which require optimization of protocol.

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