Targeted Segment Transfer from Rye Chromosome 2R to Wheat Chromosomes 2A, 2B, and 7B

2017 ◽  
Vol 151 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Tianheng Ren ◽  
Zhi Li ◽  
Benju Yan ◽  
Feiquan Tan ◽  
Zongxiang Tang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Chromosome Instability ◽  
Triticum Aestivum L ◽  
Breeding Population ◽  
Wheat Breeding ◽  
Fish Analysis ◽  
Substitution Lines ◽  
Chromosome Arm ◽  
Secale Cereale L ◽  
Translocation Lines

Increased chromosome instability was induced by a rye (Secale cereale L.) monosomic 2R chromosome into wheat (Triticum aestivum L.). Centromere breakage and telomere dysfunction result in high rates of chromosome aberrations, including breakages, fissions, fusions, deletions, and translocations. Plants with target traits were sequentially selected to produce a breeding population, from which 3 translocation lines with target traits have been selected. In these lines, wheat chromosomes 2A, 2B, and 7B recombined with segments of the rye chromosome arm 2RL. This was detected by FISH analysis using repeat sequences pSc119.2, pAs1 and genomic DNA of rye together as probes. The translocation chromosomes in these lines were named as 2ASMR, 2BSMR, and 7BSMR. The small segments that were transferred into wheat consisted of pSc119.2 repeats and other chromatin regions that conferred resistance to stripe rust and expressed target traits. These translocation lines were highly resistant to stripe rust, and expressed several typical traits that were associated with chromosome arm 2RL, which are better than those of its wheat parent, disomic addition, and substitution lines that show agronomic characteristics. The integration of molecular methods and conventional techniques to improve wheat breeding schemes are discussed.

Expression of resistance to stripe rust, powdery mildew and the wheat curl mite in Triticum aestivum × Haynaldia villosalines

2002 ◽  
Vol 82 (2) ◽  
pp. 451-456 ◽  
Author(s):  
Q. Chen ◽  
R. L. Conner ◽  
H. Li ◽  
A. Laroche ◽  
R. J. Graf ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Stripe Rust Resistance ◽  
Haynaldia Villosa ◽  
Substitution Lines ◽  
Wheat Curl Mite ◽  
Aceria Tosichella ◽  
Chromosome Arm ◽  
Progeny Tests ◽  
Translocation Lines

Stripe rust, powdery mildew and the wheat curl mite (Aceria tosichella) (WCM) are common problems in wheat throughout the world. The expression of resistance to these diseases and the mite was investigated in wheat ×Haynaldia villosa chromosome addition, substitution and translocation lines. Progeny tests and cytogenetic examinations were carried out on crosses of the 6VS translocation line with susceptible genotypes of wheat to study the inheritance of the stripe rust, powdery mildew and WCM resistance. These studies also were used to elucidate if the resistance was associated with the H. villosa 6V chromosome. The test results confirmed the presence of a high level of resistance to stripe rust in wheat × H. villosa 6V addition and 6VS translocation lines. However, progeny tests and genomic in situ hybridization (GISH) analysis showed that the stripe rust resistance gene, Yr26 was not associated with the H. villosa chromosome arm 6VS. While WCM and powdery mildew resistance were always associated with the presence of the 6VS chromosome arm in the F2 and F3 populations. The study also showed that most wheat × H. villosa addition or substitution lines were heterogenous in their reaction to stripe rust. The possible reasons for the heterogeneous response to stripe rust in wheat lines carrying the H. villosa chromosomes 2V, 3V and 4V are discussed. Key words: Haynaldia villosa, Puccinia striformis, Aceria tosichella, stripe rust, expression of resistance, addition lines, substitution lines, 6VS-translocation

scholarly journals Molecular cytogenetic identification of a wheat – Thinopyrum ponticum substitution line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (10) ◽  
pp. 860-867 ◽  
Author(s):  
Chen Zhu ◽  
Yanzhen Wang ◽  
Chunhuan Chen ◽  
Changyou Wang ◽  
Aicen Zhang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Expressed Sequence Tag ◽  
Wheat Breeding ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Thinopyrum Ponticum ◽  
Expressed Sequence

Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of JSt genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7JSt (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.

scholarly journals Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.)

Genome ◽  
2016 ◽  
Vol 59 (3) ◽  
pp. 159-165 ◽  
Author(s):  
Ling Qiu ◽  
Zong-xiang Tang ◽  
Meng Li ◽  
Shu-lan Fu
Keyword(s):  
Secale Cereale ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Addition Lines ◽  
Breeding Programs ◽  
Effective Utilization ◽  
Secale Cereale L ◽  
Monosomic Addition ◽  
Monosomic Addition Lines ◽  
Disomic Addition Lines

PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat–rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. ‘Mianyang11’ × S. cereale L. ‘Kustro’. In addition, two sets of wheat–rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring × S. cereale L. var. Imperial and T. aestivum L. ‘Holdfast’ × S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat–rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Min Zhou ◽  
Mei Du ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Breeding Population ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Development Of Resistance ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background: Lesion mimics (LM) is a disease-like symptom randomly spread on plant green leaves without any pathogens. Previous study has found that LM was related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can cause significant reduction of the quality and yield of crops. Development of resistance cultivars is economically and environmentally method for enhancing adaptability and yield stability of crops instead by fungicides application. In this study, we identify a novel LM gene with Pst resistance in wheat breeding population and mapped it with developing molecular markers for marker assisted selection (MAS) in wheat breeding.Results: In this study, a novel LM gene named as lm4 was identified by using a Yanzhan1/Neixiang188 RILs population, which closely linked to SSR markers Xgwm210 and Xgwm455 by 8.06 CM intervals. Then the genetic distance of the lm4 was shortened that flanked by SSR markers with 0.51CM and 0.77CM interval. Two SSR markers lm4_01_cib and lm4_02_cib were developed based on Chinese Spring genome database and wheat-660K SNP result, which can be used to conduct MAS of LM in wheat. And the results also show that lm4 significantly improved the resistance of stripe rust in wheat.Conclusions: Therefore, lm4 is associated with stripe rust resistance, and it may provide a new tool or theoretical support for future crop disease-resistant breeding and for understanding the plant apoptosis mechanism.

Identification and Evaluation of Wheat-Aegilops bicornis Lines with Resistance to Powdery Mildew and Stripe Rust

Plant Disease ◽  
2021 ◽  
Author(s):  
Xiaolu Wang ◽  
Ran Han ◽  
Zhiwei Chen ◽  
Jianbo Li ◽  
Tong Zhu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Stripe Rust ◽  
Powdery Mildew Resistance ◽  
Agronomic Traits ◽  
Wheat Breeding ◽  
Tiller Number ◽  
Stripe Rust Resistance ◽  
Substitution Lines ◽  
Mildew Resistance ◽  
Negative Effect

Wheat pathogens, especially those causing powdery mildew and stripe rust, seriously threaten yield worldwide. Utilizing newly identified disease resistance genes from wheat relatives is an effective strategy to minimize disease damage. In this study, chromosome-specific molecular markers for the 3Sb and 7Sb chromosomes of Aegilops bicornis were developed using PCR-based landmark unique gene (PLUG) primers for screening wheat-Ae. bicornis progenies. Fluorescence in situ hybridization (FISH) was performed to further identify wheat-Ae. bicornis progenies using oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and Oligo-(GAA)8. After establishing Ae. bicornis 3Sb and 7Sb chromosome-specific FISH markers, Holdfast (common wheat)-Ae. bicornis 3Sb addition, 7Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, 3Sb(3D) substitution, 7Sb(7A) substitution, and 7Sb(7B) substitution lines were identified by the molecular and cytological markers. Stripe rust and powdery mildew resistance, along with agronomic traits were investigated to evaluate the breeding potential of these lines. Holdfast and Holdfast-Ae. bicornis progenies were all highly resistant to stripe rust, indicating that the stripe rust resistance might derive from Holdfast. However, Holdfast-Ae. bicornis 3Sb addition, 3Sb(3A) substitution, 3Sb(3B) substitution, and 3Sb(3D) substitution lines showed high resistance to powdery mildew while Holdfast was highly susceptible, indicating that chromosome 3Sb of Ae. bicornis carries previously unknown powdery mildew resistance gene(s). Additionally, the transfer of the 3Sb chromosome from Ae. bicornis to wheat significantly increased tiller number, but chromosome 7Sb has a negative effect on agronomic traits. Therefore, wheat germplasm containing Ae. bicornis chromosome 3Sb has potential to contribute to improving powdery mildew resistance and tiller number during wheat breeding.

scholarly journals Physical Location of New Stripe Rust Resistance Gene(s) and PCR-Based Markers on Rye (Secale cereale L.) Chromosome 5 Using 5R Dissection Lines

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 498 ◽  
Author(s):  
Wei Xi ◽  
Zongxiang Tang ◽  
Jie Luo ◽  
Shulan Fu
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Secale Cereale ◽  
Rust Resistance ◽  
Wheat Breeding ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Stripe Rust Resistance Gene ◽  
Secale Cereale L

The rye (Secale cereale L.) 5R chromosome contains some elite genes that can be used to improve wheat cultivars. In this study, a set of 5RKu dissection lines was obtained, and 111 new PCR-based and 5RKu-specific markers were developed using the specific length amplified fragment sequencing (SLAF-seq) method. The 111 markers were combined with the 52 5RKu-specific markers previously reported, and 65 S. cereale Lo7 scaffolds were physically mapped to six regions of the 5RKu chromosome using the 5RKu dissection lines. Additionally, the 5RLKu arm carried stripe rust resistance gene(s) and it was located to the region L2, the same region where 22 5RKu-specific markers and 11 S. cereale Lo7 scaffolds were mapped. The stripe rust resistance gene(s) located in the 5RLKu arm might be new one(s) because its source and location are different from the previously reported ones, and it enriches the resistance source of stripe rust for wheat breeding programs. The markers and the S. cereale Lo7 scaffolds that were mapped to the six regions of the 5RKu chromosome can facilitate the utilization of elite genes on the 5R chromosome in the improvement of wheat cultivars.

scholarly journals New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds

2019 ◽  
Vol 20 (8) ◽  
pp. 2031 ◽  
Author(s):  
Wei Xi ◽  
Zongxiang Tang ◽  
Shuyao Tang ◽  
Zujun Yang ◽  
Jie Luo ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Sequences ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Fish Analysis ◽  
Breeding Programs ◽  
Gish And Fish

Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs.

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