Serum Creatine Kinase Isoenzymes in Progressive Muscular Dystrophy

Enzyme ◽  
1978 ◽  
Vol 23 (4) ◽  
pp. 238-245 ◽  
Author(s):  
Elena Tzvetanova
Keyword(s):  
Creatine Kinase ◽  
Muscular Dystrophy ◽  
Serum Creatine Kinase ◽  
Progressive Muscular Dystrophy ◽  
Creatine Kinase Isoenzymes

THE DIAGNOSTIC VALUE OF SERUM CREATINE KINASE IN NEUROMUSCULAR AND MUSCULAR DISEASE

PEDIATRICS ◽  
1965 ◽  
Vol 35 (2) ◽  
pp. 322-330
Author(s):  
Franco Vassella ◽  
Roland Richterich ◽  
Ettore Rossi
Keyword(s):  
Creatine Kinase ◽  
Muscular Dystrophy ◽  
Epileptic Seizures ◽  
Lactic Dehydrogenase ◽  
Diagnostic Value ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Progressive Muscular Dystrophy ◽  
Muscular Disease ◽  
Muscle Specificity

Serum creatine kinase activity in 200 patients was studied in order to check whether this determination might be of help in differential diagnosis between primary and secondary (neurogenic) myopathies. Elevated CK values are found physiologically in children, pregnant women, and after exercise. In pathological states increased serum enzyme concentrations (probably the expression of a defect in cellular permeability) are constantly present in the Duchenne type of progressive muscular dystrophy. More variable values are encountered in other types of muscular dystrophy and in metabolic disorders like Refsum's syndrome, coproporphyria, and hypothyroidism. In states of necrosis or injury to muscle cells like in polymyositis, dermatomyositis, after burns or trauma, serum CK concentrations vary according partly to the amount of muscle mass involved. Enzyme concentrations can be markedly increased after tetany or epileptic seizures. Normal values or occasionally slightly increased values are found in neurogenic myopathies. High CK values in patients with muscular atrophies should permit the clinician to exclude the differential diagnostic group of neurogenic myopathies. As a diagnostic tool CK proved to be more reliable than transaminases or lactic dehydrogenase because of its higher muscle specificity and a slightly greater sensitivity.

Serum creatine kinase isoenzymes in duchenne muscular dystrophy determined by sensitive enzyme immunoassay methods

1987 ◽  
Vol 10 (5) ◽  
pp. 459-463 ◽  
Author(s):  
Kenji Mokuno ◽  
Shigeo Riku ◽  
Kimiya Sugimura ◽  
Akira Takahashi ◽  
Kanefusa Kato ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Enzyme Immunoassay ◽  
Serum Creatine Kinase ◽  
Creatine Kinase Isoenzymes

Serum creatine kinase isoenzymes in duchenne muscular dystrophy

1977 ◽  
Vol 75 (3) ◽  
pp. 435-442 ◽  
Author(s):  
Takahashi Keiichi ◽  
Shutta Keiichi ◽  
Matsuo Bonpei ◽  
Takai Tsuneo ◽  
Takao. Hisashi ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Serum Creatine Kinase ◽  
Creatine Kinase Isoenzymes

Dilution Effect on Serum Creatine Kinase in Carriers of Duchenne Muscular Dystrophy

1979 ◽  
Vol 16 (1-6) ◽  
pp. 54-55 ◽  
Author(s):  
A. C. Simpson ◽  
D. Holmes ◽  
R. J. T. Pennington
Keyword(s):  
Creatine Kinase ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Dilution Effect ◽  
Serum Creatine Kinase ◽  
Normal Female ◽  
Female Subjects

Dilution of serum before assay consistently increased the activity of serum creatine kinase both in normal female subjects and in carriers of Duchenne muscular dystrophy.

scholarly journals Effect of exercise on serum creatine kinase in carriers of Duchenne muscular dystrophy.

1982 ◽  
Vol 19 (1) ◽  
pp. 4-7 ◽  
Author(s):  
R F Gaines ◽  
S M Pueschel ◽  
E A Sassaman ◽  
J L Driscoll
Keyword(s):  
Creatine Kinase ◽  
Duchenne Muscular Dystrophy ◽  
Muscular Dystrophy ◽  
Serum Creatine Kinase

Improved separation of creatine kinase isoenzymes by use of DEAE-Sepharose Cl-6B.

1980 ◽  
Vol 26 (5) ◽  
pp. 618-624 ◽  
Author(s):  
R J Bondar ◽  
D G Shevchik ◽  
M Y Hsu ◽  
M M Kohler
Keyword(s):  
Creatine Kinase ◽  
Reference Interval ◽  
Serum Creatine Kinase ◽  
Cross Contamination ◽  
Column System ◽  
Bed Height ◽  
Creatine Kinase Isoenzymes ◽  
Sepharose Column ◽  
Coefficients Of Variation ◽  
Chromatographic Procedure

Abstract We describe here a simple, rapid chromatographic procedure for quantitatively separating serum creatine kinase isoenzymes (EC 2.7.3.2), with diethylaminoethyl (DEAE)-Sepharose CL-6B as the anion-exchanger. We established the column bed height and the elution parameters by use of a simplex procedure. DEAE-Sepharose CL-6B equilibrated in tris(hydroxymethyl)aminomethane (50 mmol/L, pH 7.5, and containing 30 mmol of NaCl per liter) is packed into a miniature polystyrene column with bed dimensions of 0.7 x 1.5 cm. The DEAE-Sepharose column system was evaluated and compared with a DEAE-Sephadex A-50 column system. The results indicate that the Sepharose column yields MM, MB, and BB isoenzymes uniquely, without cross contamination. Coefficients of variation (CV’s) for 10 replicate column assays were 2.8, 5.9, and 15.2% for 569 U of MM per liter, 82.3 U of MB per liter, and 9.0 U of BB per liter, respectively. The serum sample was enriched with baboon heart extract. Day-to-day reproducibility for a serum control assayed on 10 days yielded CV’s of 4.6, 9.9, and 40.3% for 391, 45.3 and 1.9 U of isoenzymes MM, MB, and BB per liter, respectively. A reference interval for each isoenzymes was derived from data on 81 men and 63 women.

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