In vitro and in vivo Displacement of [^3H]-Diazepam Binding by Purine Derivatives in Developing Rat Brain

1984 ◽  
Vol 7 (3) ◽  
pp. 169-176 ◽  
Author(s):  
J.L. Daval ◽  
C. Barberis ◽  
P. Vert
Keyword(s):  
Rat Brain ◽  
Purine Derivatives ◽  
Developing Rat

scholarly journals Fas/CD95/APO-1 Can Function as a Death Receptor for Neuronal Cells in Vitro and in Vivo and is Upregulated Following Cerebral Hypoxic-Ischemic Injury to the Developing Rat Brain

2006 ◽  
Vol 10 (1) ◽  
pp. 17-29 ◽  
Author(s):  
Ursula Felderhoff-Mueser ◽  
Deanna L. Taylor ◽  
Kirsty Greenwood ◽  
Mary Kozma ◽  
Dietger Stibenz ◽  
...  
Keyword(s):  
Rat Brain ◽  
Neuronal Cells ◽  
Death Receptor ◽  
Ischemic Injury ◽  
Developing Rat ◽  
Hypoxic Ischemic Injury

scholarly journals Effect of hyperphenylalaninaemia on lipid synthesis from ketone bodies by rat brain

1976 ◽  
Vol 154 (2) ◽  
pp. 319-325 ◽  
Author(s):  
M S. Patel ◽  
O E. Owen
Keyword(s):  
Rat Brain ◽  
Ketone Bodies ◽  
Brain Slices ◽  
Lipid Synthesis ◽  
Coa Transferase ◽  
Old Rats ◽  
And Function ◽  
Developing Rat

The effect of hyperphenylalaninaemia on the metabolism of ketone bodies in vivo and in vitro by developing rat brain was investigated. The incorporation in vivo of [14C]acetoacetate into cerebral lipids was decreased by both chronic (for 3 days) and acute (for 6h) hyperphenylalaninaemia induced by injecting phenylalanine into 1-week-old rats. In studies in vitro it was observed that the incorporation of the radioactivity from [14C]acetoacetate and 3-hydroxy[14C]butyrate into cerebral lipids was inhibited by phenyl-pyruvate, but not by phenylalanine. Phenylpyruvate also inhibited the incorporation of 3H from 3H2O into lipids by brain slices metabolizing either 3-hydroxybutyrate or acetoacetate in the presence of glucose. These findings suggest that the decrease in the incorporation in vivo of [14C]acetoacetate into cerebral lipids in hyperphenylalaninaemic rats is most likely caused by phenylpyruvate and not by phenylalanine. Phenylpyruvate as well as phenylalanine had no inhibitory effects on ketone-body-catabolizing enzymes, namely 3-hydroxybutyrate dehydrogenase, 3-oxo acid CoA-transferase and acetoacetyl-CoA thiolase, in rat brain. Phenylpyruvate but not phenylalanine inhibited the activity of the 2-oxoglutarate dehydrogenase complex from rat and human brain. These findings suggest that the metabolism of ketone bodies is impaired in brains of untreated phenylketonuric patients, and in turn may contribute to the diminution of mental development and function associated with phenylketonuria.

scholarly journals Carbodiimide as a tissue fixative in histamine immunohistochemistry and its application in developmental neurobiology.

1988 ◽  
Vol 36 (3) ◽  
pp. 259-269 ◽  
Author(s):  
P Panula ◽  
O Häppölä ◽  
M S Airaksinen ◽  
S Auvinen ◽  
A Virkamäki
Keyword(s):  
Rat Brain ◽  
Nerve Fibers ◽  
Immunohistochemical Method ◽  
Dot Blot ◽  
Culture Dish ◽  
Long Fibers ◽  
Developing Rat ◽  
Strong Immunoreactivity

The object of this study was to develop an immunohistochemical method that could be used to study neuronal histamine, especially in nerve fibers and terminals where most previous methods have not been applicable. Three new antisera were produced in rabbits against conjugated histamine, and the fixative used in conjugation, 1-ethyl-3(3-diamethylaminopropyl)-carbodiimide (EDCDI), was used in tissue fixation and compared to paraformaldehyde. Specificity of the antisera was established with dot-blot tests on nitrocellulose, with blocking controls and affinity-purified antibodies. EDCDI appeared to be superior to paraformaldehyde as a fixative, and histamine-immunoreactive nerve cells were visualized in developing rat brain during late fetal development from embryonal day 12. By the second postnatal week, the distribution of histamine-immunoreactive neurons in rat brain had reached the adult pattern and immunoreactive nerve fibers were seen in many areas. Posterior hypothalamic neurons from newborn rat in vitro showed strong immunoreactivity for histamine and developed long varicose fibers, which covered the culture dish by the end of the fourth week in vitro. Fixation with EDCDI also allowed detection of histamine in gastric enterochromaffin-like cells and mast cells in rat. The results suggest that the histamine-containing neuron system in rat brain develops during the late fetal and early postnatal periods, and that immunoreactive neurons develop long fibers both in vivo and in vitro.

scholarly journals Angiogenesis in developing rat brain: An in vivo and in vitro study

1985 ◽  
Vol 23 (2) ◽  
pp. 219-223 ◽  
Author(s):  
Patricia L. Robertson ◽  
Monica Du Bois ◽  
Phillip D. Bowman ◽  
Gary W. Goldstein
Keyword(s):  
Rat Brain ◽  
In Vitro Study ◽  
Vitro Study ◽  
Developing Rat

scholarly journals Phosphorylation-dependent interaction of the synaptic vesicle proteins cysteine string protein and synaptotagmin I

2002 ◽  
Vol 364 (2) ◽  
pp. 343-347 ◽  
Author(s):  
Gareth J.O. EVANS ◽  
Alan MORGAN
Keyword(s):  
Rat Brain ◽  
Direct Interaction ◽  
Secretory Vesicle ◽  
Brain Membrane ◽  
Synaptotagmin I ◽  
Phosphorylated Form ◽  
Order Of Magnitude ◽  
And Function

The secretory vesicle cysteine string proteins (CSPs) are members of the DnaJ family of chaperones, and function at late stages of Ca2+-regulated exocytosis by an unknown mechanism. To determine novel binding partners of CSPs, we employed a pull-down strategy from purified rat brain membrane or cytosolic proteins using recombinant hexahistidine-tagged (His6-)CSP. Western blotting of the CSP-binding proteins identified synaptotagmin I to be a putative binding partner. Furthermore, pull-down assays using cAMP-dependent protein kinase (PKA)-phosphorylated CSP recovered significantly less synaptotagmin. Complexes containing CSP and synaptotagmin were immunoprecipitated from rat brain membranes, further suggesting that these proteins interact in vivo. Binding assays in vitro using recombinant proteins confirmed a direct interaction between the two proteins and demonstrated that the PKA-phosphorylated form of CSP binds synaptotagmin with approximately an order of magnitude lower affinity than the non-phosphorylated form. Genetic studies have implicated each of these proteins in the Ca2+-dependency of exocytosis and, since CSP does not bind Ca2+, this novel interaction might explain the Ca2+-dependent actions of CSP.

In vivo and in vitro Study of V Uptake in Developing Rat Molar Enamel

1980 ◽  
Vol 59 (10) ◽  
pp. 1643-1648 ◽  
Author(s):  
J.W. Bawden ◽  
T.G. Deaton ◽  
M. Chavis
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Developing Rat ◽  
Rat Molar

In vitro and in vivo depolarization coupled efflux of ascorbic acid in rat brain preparations

1981 ◽  
Vol 7 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Kristin H. Milby ◽  
Ivan N. Mefford ◽  
Willie Chey ◽  
Ralph N. Adams
Keyword(s):  
Ascorbic Acid ◽  
Rat Brain
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