The Lack of Association between FCN2 Gene Promoter Region Polymorphisms and Dental Caries in Polish Children

2017 ◽  
Vol 51 (1) ◽  
pp. 79-84 ◽  
Author(s):  
T. Olszowski ◽  
M. Milona ◽  
J. Janiszewska-Olszowska ◽  
K. Safranow ◽  
K. Skonieczna-Żydecka ◽  
...  
Keyword(s):  
Dental Caries ◽  
Promoter Region ◽  
Gene Promoter ◽  
Caries Experience ◽  
Nucleotide Polymorphisms ◽  
Promoter Polymorphisms ◽  
Gene Encoding ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Gene Promoter Region

The aim of this study was to examine the association of single-nucleotide polymorphisms (SNPs) in the gene encoding ficolin-2 protein (FCN2 gene) at positions -986 (rs17514136), -602 (rs3124953), and -4 (rs3124952) with dental caries in Polish children. Two hundred and sixty Polish Caucasian children aged 15 years were enrolled in this study: 82 with “higher” caries experience (DMFT >5) and 178 with “lower” caries experience (DMFT ≤5). In addition, subjects with caries experience (DMFT ≥1) and caries-free subjects (DMFT = 0) were compared. FCN2 SNPs were genotyped with PCR-RFLP methods. There were no significant differences in the genotype, allele, or haplotype distributions in 3 analyzed SNPs of the FCN2 gene between children with “higher” and those with “lower” caries experience as well as between children with caries experience and caries-free children. In conclusion, we did not find any association of FCN2 promoter polymorphisms at positions -986, -602, and -4 with dental caries in Polish children.

scholarly journals Study of the Relationship between Polymorphisms in the IL-8 Gene Promoter Region and Coccidiosis Resistance Index in Jinghai Yellow Chickens

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 476
Author(s):  
Xiao-Hui Wang ◽  
Hai-Liang Yu ◽  
Wen-Bin Zou ◽  
Chang-Hao Mi ◽  
Guo-Jun Dai ◽  
...  
Keyword(s):  
Promoter Region ◽  
Interleukin 2 ◽  
Gene Promoter ◽  
Resistance Index ◽  
Interferon Γ ◽  
P Value ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Ifn Γ ◽  
Gene Promoter Region

Interleukin 8 (IL-8) participates in the immune response and has the function of inducing neutrophils to release lysosomal enzymes and eliminate pathogens. This study was to investigate the effect of single nucleotide mutations in the IL-8 gene promoter region on the coccidiosis resistance index. In this study, 180 infected Eimeria tenella (E. tenella) Jinghai yellow chickens were used as experimental samples. DNA sequencing technology was used to detect single nucleotide polymorphisms (SNPs) in the IL-8 gene promoter region. The association between these SNPs and coccidiosis resistance indexes (including superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX), catalase (CAT), nitric oxide (NO), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), IL-8, and interferon-γ (IFN-γ)) were analyzed. Three SNPs (T-550C, G-398T, and T-360C) were detected. Significant associations were found between each genotype at the T-550C site with NO (p-value = 0.006) and IL-8 (p-value = 0.034) indexes. Significant associations were found between each genotype at the G-398T site with SOD (p-value = 0.042), CAT (p-value = 0.049), NO (p-value = 0.008), and IL-2 (p-value = 0.044) indexes. Significant associations were found between each genotype at the T-360C site with SOD (p-value = 0.007), NO (p-value = 0.046), IL-2 (p-value = 0.041), IL-8 (p-value = 0.039), and IFN-γ (p-value = 0.042) indexes. Haplotype analysis showed that multiple indexes of the H1H3 haplotype combination were significantly higher than other haplotype combinations. Therefore, mutation of the IL-8 gene promoter region has a significant regulatory effect on the coccidiosis resistance index, with a change in transcription factor binding potentially altering IL-8 gene expression, thereby further affecting the IL-8 level in plasma. However, the specific mechanism needs further study.

scholarly journals The Escherichia coli serS gene promoter region overlaps with the rarA gene

2021 ◽  
Author(s):  
Kanika Jain ◽  
Tyler H. Stanage ◽  
Elizabeth A. Wood ◽  
Michael M. Cox
Keyword(s):  
Escherichia Coli ◽  
Promoter Region ◽  
Gene Promoter ◽  
Stringent Response ◽  
Trna Synthetase ◽  
Growth Defect ◽  
Aminoacyl Trna Synthetase ◽  
Gene Encoding ◽  
Gene Promoter Region ◽  
Entire Gene

Deletion of the entire gene encoding the RarA protein of Escherichia coli results in a growth defect and additional deficiencies that were initially ascribed to a lack of RarA function. Further work revealed that most of the effects reflected the presence of sequences in the rarA gene that affect expression of the downstream gene, serS. The serS gene encodes the seryl aminoacyl-tRNA synthetase. Decreases in the expression of serS can trigger the stringent response. The sequences that affect serS expression are located in the last 15 nucleotides of the rarA gene.

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