Cariogenic Potential of Sucrose Associated with Maltodextrin on Dental Enamel

2017 ◽  
Vol 51 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Gabriela Rezende ◽  
Rodrigo A. Arthur ◽  
Debora Grando ◽  
Lina N. Hashizume
Keyword(s):  
Dental Enamel ◽  
Experimental Period ◽  
Mutans Streptococci ◽  
Extracellular Polysaccharides ◽  
Double Blind Study ◽  
Double Blind ◽  
Dextrose Equivalent ◽  
Inorganic Composition ◽  
Bovine Enamel

Maltodextrin is a hydrolysate of cornstarch and has been widely used in the food industry associated with sucrose. The addition of starch can increase the cariogenic potential of sucrose; however, there are sparse data regarding the cariogenicity of sucrose associated with maltodextrin. Therefore, the aim of this study was to test in situ if maltodextrin could increase the cariogenic potential of sucrose. This was an in situ, randomized, crossover, split-mouth, and double-blind study. Volunteers wore palatal appliances containing bovine enamel blocks for 2 periods of 14 days. They dripped the following solutions on the enamel blocks 8 times per day: deionized distilled water (DDW), maltodextrin (M), sucrose + maltodextrin (S+M), or sucrose (S). At the end of each experimental period, biofilms were collected and analyzed for microbiological (mutans streptococci, lactobacilli, and total microorganisms counts) and biochemical (calcium, inorganic phosphate, fluoride, and insoluble extracellular polysaccharides concentrations) compositions. The enamel demineralization was assessed by microhardness. Treatments S and S+M resulted in a lower inorganic composition and higher concentration of insoluble extracellular polysaccharides in the biofilms, and higher enamel mineral loss compared to DDW and M. It can be concluded that the cariogenic potential of sucrose is not changed when this carbohydrate is associated with maltodextrin (dextrose equivalent 13-17).

In situ Effect of Frequent Sucrose Exposure on Enamel Demineralization and on Plaque Composition after APF Application and F Dentifrice Use

2004 ◽  
Vol 83 (1) ◽  
pp. 71-75 ◽  
Author(s):  
A.F. Paes Leme ◽  
R. Dalcico ◽  
C.P.M. Tabchoury ◽  
A.A. Del Bel Cury ◽  
P.L. Rosalen ◽  
...  
Keyword(s):  
Sucrose Solution ◽  
Experimental Period ◽  
Mutans Streptococci ◽  
Plaque Composition ◽  
Treatment Groups ◽  
Enamel Demineralization ◽  
Combination Of Methods ◽  
Bovine Enamel ◽  
Mineral Loss

Since the effect of the combination of methods of fluoride use on enamel demineralization and on plaque composition is not clearly established, this study examined the effect of the combination of acidulated phosphate fluoride (APF) application and F dentifrice on enamel demineralization and on plaque composition. In this crossover study, 16 volunteers, wearing a palatal appliance containing bovine enamel blocks, were subjected to 4 treatment groups: non-fluoridated dentifrice (PD), FD, APF+PD, and APF+FD. The APF was applied to the enamel before the 14-day experimental period. During the experimental period, test dentifrices were applied 3×/day, and a 20% sucrose solution was applied 4× and 8×/day by being dripped on the blocks. Although APF application was able either to increase F concentration in plaque or to reduce the % of mutans streptococci, its combination with F dentifrice use neither reduced enamel mineral loss nor changed any other measured plaque variable with respect to the FD group alone.

Safety and In Situ Antierosive Effect of CaneCPI-5 on Dental Enamel

2021 ◽  
pp. 002203452110115
Author(s):  
V.T. Pelá ◽  
J.G.Q. Lunardelli ◽  
C.K. Tokuhara ◽  
C.C. Gironda ◽  
N.D.G. Silva ◽  
...  
Keyword(s):  
Dental Enamel ◽  
Erosive Wear ◽  
Tooth Wear ◽  
Positive Control ◽  
Negative Control ◽  
Mitochondrial Activity ◽  
Double Blind ◽  
Enamel Erosion ◽  
Bovine Enamel

The sugarcane cystatin (CaneCPI-5) was recently cloned and showed strong binding force to dental enamel and protection against initial erosion. However, evaluations on its safety and efficacy in a situation closer to the clinical condition are necessary. In the present study we analyzed 1) the cytotoxicity of CaneCPI-5 on human gingival fibroblasts (HGFs); 2) the ability of CaneCPI-5 to reduce enamel erosion and erosion+abrasion in situ. In part 1, HGFs were treated with CaneCPI-5 (0.025, 0.05, 0.1, 0.5 or 1.0 mg/mL) or no treatment (control). The cytotoxicity was assessed after 60 s and 24 h by mitochondrial activity (MTT), confocal microscopy, and hematoxylin/eosin staining. In part 2, 15 volunteers participated in a double-blind crossover protocol consisting of 3 phases, according to the following treatments: 1) 0.1 mg/mL CaneCPI-5; 2) SnCl2/NaF/AmF (Elmex; positive control); 3) water (negative control). The volunteers wore an appliance containing 4 bovine enamel specimens for 5 d. Each day, the specimens were individually treated with 50 µL of the tested solutions per 60 s and then subjected to erosive challenges (0.1% citric acid, pH 2.5, for 90 s, 4 times per day). After the first and last erosive challenge each day, 2 samples were abraded (toothbrushing, 15 s). Enamel wear was measured by contact profilometry. One or two-way analysis of variance (ANOVA)/Tukey’s or Sidak’s tests ( P < 0.05) were applied. Regardless of the concentration and the experimental time, CaneCPI-5 did not decrease the cell viability compared to the negative control ( P < 0.05). Erosion+abrasion led to significantly greater wear compared to erosion only. For both conditions, the lowest wear was found for SnCl2 and CaneCPI-5, which did not differ significantly from each other, but showed significant protection when compared to the negative control. In conclusion, CaneCPI-5 is safe on HGFs and reduces enamel erosive wear to the same extent as a commercial solution used to control erosive tooth wear (ETW).

Effect of a Nitroglycerin Patch on Perfusion to the Foot in Healthy Subjects

2006 ◽  
Vol 96 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Paul Jeong Kim ◽  
L. Clay Ballinger ◽  
Donald Kushner
Keyword(s):  
Nitric Oxide ◽  
Healthy Subjects ◽  
Healing Process ◽  
Experimental Period ◽  
Drug Group ◽  
Wound Healing Process ◽  
Double Blind Study ◽  
Double Blind ◽  
Adhesive Patch ◽  
The Subject

Nitric oxide is an endogenous gas released by endothelial cells that induces vasodilatation and plays other important roles in the wound-healing process. Nitroglycerin preparations are liberators of nitric oxide. Podiatric physicians have used nitroglycerin paste and patches on patients in an attempt to increase perfusion to the foot. However, the drug’s efficacy seems to be largely anecdotal. A prospective, randomized, placebo-controlled, double-blind study was conducted to investigate the efficacy of a nitroglycerin patch in locally increasing perfusion to the foot. Twenty-two healthy subjects were randomly assigned to either a drug group (nitroglycerin patch, 0.2 mg/h) or a placebo group (adhesive patch without active ingredient). The patch was applied to the plantar arch of the foot. Objective and subjective measures were then used to detect changes in perfusion to the foot after a 2-hour experimental period. The objective measures, cutaneous thermometry and photoplethysmography, found no significant measurable difference in perfusion to the foot between the drug and placebo groups (P &gt; .05). A subjective questionnaire used to assess changes in temperature or sensation detected by the subject yielded similar results. Thus a nitroglycerin patch dose of 0.2 mg/h showed no measurable ability to increase perfusion to the foot. Further research is needed to validate the indications for this therapy. (J Am Podiatr Med Assoc 96(4): 318–322, 2006)

scholarly journals In situ Assessment of Effects of the Bromide- and Fluoride-incorporating Adhesive Systems on Biofilm and Secondary Caries

2014 ◽  
Vol 15 (2) ◽  
pp. 142-148 ◽  
Author(s):  
Suyane Maria Luna Cruz de Vasconcelos ◽  
Mary Anne Sampaio de Melo ◽  
Joao Paulo Marques Saraiva Wenceslau ◽  
Iriana Carla Junqueira Zanin ◽  
Haroldo Cesar Pinheiro Beltrao ◽  
...  
Keyword(s):  
Mutans Streptococci ◽  
Adhesive Systems ◽  
Double Blind ◽  
Secondary Caries ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Significant Difference ◽  
In Situ Assessment ◽  
Self Etch

ABSTRACT Aim This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization. Materials and methods During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SETM (self-etch, fluoride/MDPB free adhesive, AB) and Clearfil Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S (ƒ¢S) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 ƒÊm from the restoration margin. Data were analyzed by ANOVA. Results No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm. Conclusion All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research. How to cite this article de Vasconcelos SMLC, de Melo MAS, Wenceslau JPMS, Zanin ICJ, Beltrao HCP, Fernandes CAO, de Almeida PC, Rodrigues LKA. In situ Assessment of Effects of the Bromide- and Fluoride-Incorporating Adhesive Systems on Biofilm and Secondary Caries. J Contemp Dent Pract 2014;15(2):142-148.

scholarly journals Influence of subinhibitory concentrations of extracts from Psidium cattleianum (Sabine) and Myracrodruon urundeuva (Allemao) on mutans streptococci adhesion to glass and enamel surfaces.

2017 ◽  
Vol 6 (3) ◽  
Author(s):  
Karina Gerhardt Bianco ◽  
Luis Fernando Landucci ◽  
Christiane Marie Schweitzer ◽  
Ellen Cristina Gaetti-Jardim ◽  
Elerson Gaetti-Jardim Júnior
Keyword(s):  
Control Plant ◽  
Dental Enamel ◽  
Test Group ◽  
Mutans Streptococci ◽  
Dilution Method ◽  
Adhesion Assay ◽  
Bacterial Strains ◽  
Psidium Cattleianum ◽  
Subinhibitory Concentrations ◽  
Bovine Enamel

This study evaluated the influence of subinhibitory concentrations of extracts from Myracrodruon urundeuva and Psidium cattleianum on the adhesion of cariogenic cocci to the surface of glass and bovine enamel. The aqueous extracts were prepared from leaves by heating in deionized water and sterilized by filtration (22μm). The minimum inhibitory concentration (MIC) of the extracts on S. mutans ATCC 1910 and ATCC 35688 was determined by the broth dilution method. In the tests of adhesion to glass surfaces, glass tubes were treated with saliva and sucrose and the bacterial strains were cultivated in concentrations equivalent to 25% of MIC, which were also maintained in the adhesion assay. After the contact between the cells and the glass, the unbound cocci were removed and, finally, it was performed the removal of the cells adhered to the glass by sonication. The percentage of adhesion was determined by comparing the number of bacteria adhered to the total inoculated bacteria, in the presence and absence of the subinhibitory concentrations of the extracts. For enamel adhesion tests, bovine enamel samples were standardized, polished, treated with saliva and, in the test group, received the bacterial inoculum and 25% of the MIC of the extracts. The enamel blocks were removed and the total of adhered cocci was determined by cultivation on BHI agar supplemented with horse blood in anaerobic conditions for 48h at 37oC. The results were evaluated through analysis of variance (ANOVA). The extracts were effective in inhibiting bacterial adhesion to glass and dental enamel surfaces, although inhibition varied according to bacterial strain and extract, producing from 40 to 60% of inhibition. The results reinforce the possibility of the use of extracts of plants of the Brazilian savanna in the control of cariogenic biofilms.Descriptors: Bacteria; Prevention & Control; Plant Extracts; Dental Caries.

Effect of an Experimental Paste with Hydroxyapatite Nanoparticles and Fluoride on Dental Demineralisation and Remineralisation in situ

2015 ◽  
Vol 49 (5) ◽  
pp. 499-507 ◽  
Author(s):  
Beatriz M. Souza ◽  
Livia P. Comar ◽  
Mariele Vertuan ◽  
Constantino Fernandes Neto ◽  
Marília Afonso Rabelo Buzalaf ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Positive Influence ◽  
Double Blind Study ◽  
Double Blind ◽  
Mi Paste Plus ◽  
Mi Paste ◽  
Fluoride Dentifrice ◽  
Casein Phosphopeptide ◽  
Mineral Loss

This study evaluated the effect of an experimental paste containing hydroxyapatite in nanoparticles (nano-HA)/fluoride on dental de-remineralisation in situ. Thirteen subjects took part in this crossover/randomised/double-blind study performed in 4 phases (14 days each). Four sound and 4 pre-demineralised specimens were worn intraorally at each phase corresponding to the following treatments: Nanop Plus (10% HA, 0.2% NaF, nano-HA/fluoride), MI Paste Plus (casein phosphopeptide-amorphous calcium phosphate, 0.2% NaF), F (0.2% NaF) and placebo. Two-hundred and forty enamel and 240 dentine specimens were selected by using surface microhardness; half of them were subjected to pre-demineralisation and the other half remained sound. Sound specimens were further exposed to severe cariogenic challenge (20% sucrose in biofilm) in situ, while pre-demineralised specimens were not. All specimens were exposed to fluoride dentifrice slurry 2 × 1 min/day. Thereafter, the treatments were done for 4 min. The de-remineralisation was quantified by transversal microradiography. The data were statistically analysed by repeated-measures ANOVA/Tukey's tests (p < 0.05). Generally, no huge differences were found among the treatments. However, Nanop Plus was the only treatment able to significantly reduce dentine demineralisation (ΔZ, integrated mineral loss) and to improve enamel remineralisation (ΔΔZ, integrated mineral uptake) compared to placebo. No treatments were able to reduce enamel demineralisation, while for dentine remineralisation all treatments were similarly effective in improving ΔΔZ compared to placebo. Nanop Plus seems to have a positive influence on dental de-remineralisation, which should be further confirmed.

Effectiveness of a Toothpaste with Low Fluoride Content Combined with Trimetaphosphate on Dental Biofilm and Enamel Demineralization in situ

2015 ◽  
Vol 49 (4) ◽  
pp. 394-400 ◽  
Author(s):  
Eliana M. Takeshita ◽  
Marcelle Danelon ◽  
Luciene P. Castro ◽  
Kikue T. Sassaki ◽  
Alberto C.B. Delbem
Keyword(s):  
Sucrose Solution ◽  
Blind Study ◽  
Double Blind Study ◽  
Oral Appliances ◽  
Double Blind ◽  
Sodium Trimetaphosphate ◽  
Dental Biofilm ◽  
Enamel Demineralization ◽  
Mineral Loss

Objective: The aim of the present study was to evaluate in situ whether a toothpaste with low fluoride associated with sodium trimetaphosphate (TMP) would provide similar effect to that of a 1,100 ppm F toothpaste. Design: This crossover double-blind study consisted of 4 phases (14 days each), during which 10 volunteers wore oral appliances containing 4 enamel bovine blocks. The cariogenic challenge was performed by the application of a 20% sucrose solution (6×/day). The toothpaste treatments (2×/day) were: placebo, 500 ppm F, 500 ppm F plus 1% TMP, and 1,100 ppm F. At the end, enamel mineral loss and biofilm composition were analyzed. Results: The toothpaste with 500 ppm F plus 1% TMP showed the lowest mineral loss (p < 0.05). Regarding the fluoride and calcium concentrations in the enamel and in the biofilm, there were no significant differences between 500 ppm F plus 1% TMP, and 1,100 ppm F toothpastes (p > 0.569), but they were significantly different when compared to toothpaste with 500 ppm F (p < 0.050). Conclusion: The addition of 1% TMP to a low-fluoride toothpaste reduces enamel demineralization in situ similar to a 1,100 ppm F toothpaste.

The Effect of Copper on Demineralization of Dental Enamel

2006 ◽  
Vol 85 (11) ◽  
pp. 1011-1015 ◽  
Author(s):  
A.Z. Abdullah ◽  
S.M. Strafford ◽  
S.J. Brookes ◽  
M.S. Duggal
Keyword(s):  
Dental Enamel ◽  
Double Blind ◽  
Fluoride Treatment ◽  
Before And After ◽  
Amine Fluoride ◽  
Knoop Microhardness ◽  
Effect Of Copper ◽  
Removable Appliance

Previous studies have concluded that copper might inhibit enamel demineralization in vitro. Our aim was to assess the effect of copper (Cu2+), with and without amine fluoride, on human dental enamel under cariogenic challenge in situ. In a double-blind randomized four-leg crossover trial, 14 individuals wore a removable appliance containing 2 enamel slabs, 1 containing an artificial caries lesion. During each leg, the appliance was exposed twice daily to one of the test solutions: 1.25 mM CuSO4, amine fluoride (250 ppm F), copper and amine fluoride combined, or a placebo (water). A cariogenic challenge was provided in all cases by 5 daily exposures to 10% sucrose. Slabs were assessed before and after 21 days’ exposure by Knoop microhardness and transverse microradiography. Significantly less demineralization was observed with Cu2+ and fluoride in combination than with fluoride treatment alone (p < 0.05), whereas copper alone had no significant protective effect.

scholarly journals Effect of plaque accumulation and salivary factors on enamel demineralization and plaque composition in situ

2003 ◽  
Vol 17 (4) ◽  
pp. 326-331 ◽  
Author(s):  
Livia Maria Andaló Tenuta ◽  
José Eduardo de Oliveira Lima ◽  
Celso Luiz Cardoso ◽  
Cínthia Pereira Machado Tabchoury ◽  
Jaime Aparecido Cury
Keyword(s):  
Sucrose Solution ◽  
Salivary Secretion ◽  
Mutans Streptococci ◽  
Plaque Composition ◽  
Surface Microhardness ◽  
Enamel Demineralization ◽  
Caries Progression ◽  
Baseline Values ◽  
Bovine Enamel

This study evaluated the effect of some plaque and salivary factors on caries progression in situ. The salivary secretion rate, buffering capacity and mutans streptococci counts from 13 volunteers were determined. For three distinct periods of time, 4, 7 and 10 days, each of them wore a palatal appliance containing 4 bovine enamel blocks. They used a non-fluoridated dentifrice during the experiment and a 20% sucrose solution was dripped onto the blocks 10 times a day. Mutans streptococci (MS), calcium (Ca), and insoluble polysaccharide (IP) were quantified in the dental plaque formed on the enamel blocks, after each period. Enamel demineralization was assessed by surface microhardness, and the percentage of surface microhardness change (%SMC) in relation to the baseline values was calculated. Enamel demineralization occurred after each period of plaque accumulation (p < 0.05), and the %SMC increased with time (from 13.8 to 48.3%). The concentrations of Ca and IP in plaque were not statistically different among the experimental times, but significant correlations were found between these concentrations and %SMC. Neither the salivary factors assessed initially nor mutans streptococci in plaque presented statistically significant correlations to %SMC. The results suggest that enamel demineralization is time-dependent and is more related to the composition of the biofilm formed than to the salivary factors studied.

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