‘Six Memos' for Lymph Node Fine-Needle Cytology and Flow Cytometry

2016 ◽  
Vol 60 (4) ◽  
pp. 281-283 ◽  
Author(s):  
Pio Zeppa
Keyword(s):  
Flow Cytometry ◽  
Lymph Node ◽  
Fine Needle ◽  
Fine Needle Cytology

scholarly journals Lymph Node Fine-Needle Cytology: Beyond Flow Cytometry

2016 ◽  
Vol 60 (4) ◽  
pp. 372-384 ◽  
Author(s):  
Anna Lucia Peluso ◽  
Antonio Ieni ◽  
Chiara Mignogna ◽  
Pio Zeppa
Keyword(s):  
Flow Cytometry ◽  
In Situ Hybridization ◽  
Lymph Node ◽  
New Technologies ◽  
Cell Receptor ◽  
Diagnostic Tools ◽  
Comparative Genomic ◽  
Fine Needle ◽  
Fine Needle Cytology

Lymph node (LN) fine-needle cytology (FNC) coupled with flow cytometry immunophenotyping provides relevant information for the diagnosis of non-Hodgkin lymphoma (NHL). Numerous studies have shown FNC samples to be suitable for different molecular procedures; in this review, some of the molecular procedures most commonly employed for NHL are briefly described and evaluated in this perspective. Fluorescence in situ hybridization and chromogenic in situ hybridization are briefly described. Polymerase chain reaction (PCR)-based assays are used to identify and quantify mutations and translocations, namely immunoglobulin (IGH) and T-cell receptor rearrangements by clonality testing and IGVH somatic hypermutations either by Sanger sequencing, single-strand conformational polymorphisms or RT-PCR strategies. High-throughput technologies (HTT) encompass numerous and different diagnostic tools that share the capacity of multiple molecular investigation and sample processing in a fast and reproducible manner. HTT includes gene expression profiling, comparative genomic hybridization, single-nucleotide polymorphism arrays and next-generation sequencing technologies. A brief description of these tools and their potential application to LN FNC is reported. The challenge for FNC will be to achieve new knowledge and apply new technologies to FNC, exploiting its own basic qualities.

Lymph Node Fine-Needle Cytology of Non-Hodgkin Lymphoma: Diagnosis and Classification by Flow Cytometry

2016 ◽  
Vol 60 (4) ◽  
pp. 302-314 ◽  
Author(s):  
Immacolata Cozzolino ◽  
Monia Rocco ◽  
Giancarlo Villani ◽  
Marco Picardi
Keyword(s):  
Flow Cytometry ◽  
Lymph Node ◽  
Detailed Analysis ◽  
Hodgkin Lymphoma ◽  
Diagnostic Algorithm ◽  
Fine Needle ◽  
Fine Needle Cytology ◽  
Non Hodgkin Lymphoma ◽  
Diagnosis And Classification

In the last decades, lymph node fine-needle cytology (FNC), coupled with flow cytometry (FC), has gained a role in the diagnosis and classification of non-Hodgkin lymphoma (NHL). The combination of FNC/FC allows the diagnosis and classification of NHL in lymph node samples with a high sensitivity and specificity by combining cytological features and specific phenotypic profiles. The present review provides a brief technical description of FC and a detailed analysis of the current markers and their combinations (diagnostic algorithm) for the diagnosis and classification of NHL. The basic principles of clonality assessment, as well as the diagnostic strengths and weaknesses of the procedure, are reported. The current diagnostic algorithms for NHL classification are critically reviewed with a focus on specific problems related to single entities. Moreover, this review provides a detailed analysis of the different clinical contexts in which FNC/FC is performed and related implications. Future and further applications of FNC/FC for NHL are also discussed.

scholarly journals Lymph node fine needle Cytology in the staging and follow-up of Cutaneous Lymphomas

BMC Cancer ◽  
2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Elena Vigliar ◽  
Immacolata Cozzolino ◽  
Marco Picardi ◽  
Anna Lucia Peluso ◽  
Laura Virginia Sosa Fernandez ◽  
...  
Keyword(s):  
Lymph Node ◽  
Fine Needle ◽  
Fine Needle Cytology ◽  
Cutaneous Lymphomas

Fine-Needle Cytology and Flow Cytometry Assessment of Reactive and Lymphoproliferative Processes of the Breast

2012 ◽  
Vol 56 (2) ◽  
pp. 130-138 ◽  
Author(s):  
Elena Vigliar ◽  
Immacolata Cozzolino ◽  
Laura Virginia Sosa Fernandez ◽  
Leda Di Pietto ◽  
Albina Riccardi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fine Needle ◽  
Fine Needle Cytology

scholarly journals Cytologic, flow cytometry, and molecular assessment of lymphoid infiltrate in fine-needle cytology samples of Hashimoto thyroiditis

2009 ◽  
Vol 117 (3) ◽  
pp. 174-184 ◽  
Author(s):  
Pio Zeppa ◽  
Immacolata Cozzolino ◽  
Anna Lucia Peluso ◽  
Giancarlo Troncone ◽  
Antonio Lucariello ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Hashimoto Thyroiditis ◽  
Fine Needle ◽  
Lymphoid Infiltrate ◽  
Fine Needle Cytology ◽  
Molecular Assessment

Combined fine needle cytology and flow cytometry immunophenotyping for diagnosis of lymphoid disorders

2008 ◽  
Vol 49 (6) ◽  
pp. 1212-1213
Author(s):  
Giovanni D'Arena ◽  
Nicola Cascavilla ◽  
Maria Napolitano ◽  
Gaetano Corazzelli ◽  
Franco Fulciniti
Keyword(s):  
Flow Cytometry ◽  
Fine Needle ◽  
Fine Needle Cytology

Lymph node fine‐needle cytology in the era of personalised medicine. Is there a role?

Cytopathology ◽  
2019 ◽  
Vol 30 (4) ◽  
pp. 348-362 ◽  
Author(s):  
Immacolata Cozzolino ◽  
Valentina Giudice ◽  
Chiara Mignogna ◽  
Carmine Selleri ◽  
Alessandro Caputo ◽  
...  
Keyword(s):  
Lymph Node ◽  
Personalised Medicine ◽  
Fine Needle ◽  
Fine Needle Cytology

scholarly journals Is Flow Cytometry Alone Enough in the Assessment of Lymph Node Fine-Needle Aspirations?

2018 ◽  
Vol 150 (suppl_1) ◽  
pp. S83-S83
Author(s):  
Alexandria Smith-Hannah ◽  
Rana Naous
Keyword(s):  
Flow Cytometry ◽  
Lymph Node ◽  
Fine Needle

Fine‐needle cytology of intraglandular parotid lymph node: A useful procedure in the management of salivary gland nodules

2019 ◽  
Author(s):  
Giulio Vitagliano ◽  
Giuseppe Santoro ◽  
Luigi Landolfi ◽  
Immacolata Cozzolino ◽  
Anna L. Peluso ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Lymph Node ◽  
Fine Needle ◽  
Fine Needle Cytology
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